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Method for stabilizing liquid photosynthetic bacterium living bacterium preparation

A technology of living bacteria preparation and photosynthetic bacteria, applied in the field of microbiology, can solve the problem of invisible preparation sedimentation and stratification, and achieve the effects of preventing bacterial aggregation and sedimentation, formulation stability, and solving bacterial sedimentation and stratification.

Inactive Publication Date: 2018-12-18
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, opaque containers are often used to fill photosynthetic bacteria liquid preparations, or cover the outer surface of the container with label paper, and the sedimentation and stratification of the preparation cannot be seen apparently, and this problem has not been fundamentally solved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of method of stabilizing liquid photosynthetic bacteria preparation of the present invention, in 10L specification transparent glass reactor, add 2.0L distilled water, in room temperature environment, under stirring condition, after adding 75.0g NaCl to dissolve, then slowly (a small amount of multiple times) add 50.0g xanthan gum to form a xanthan gum mixture. The amount added each time should be completely dissolved. The xanthan gum mixture is continuously stirred and heated to 50°C, and then stirred for 10 minutes until the xanthan gum is completely dissolved, forming Xanthan gum solution, the xanthan gum solution is sterilized by high-pressure steam at 121°C for 30 minutes, and naturally cooled to a temperature not higher than 42°C after sterilization to obtain a sterile polymeric organic medium solution. Supplement sterile water to the 2.50L mark, stir evenly, the xanthan gum mass volume concentration (hereinafter referred to as concentration) in the reactor ...

Embodiment 2

[0024] A kind of method of stabilizing liquid photosynthetic bacteria preparation of the present invention, in the beaker that is equipped with about 750mL distilled water, in room temperature environment, under magnetic stirring, add 24.00g NaCl, 9.60g xanthan gum, 2.40g welan gum and 2.40g Gelatin, to form a mixed solution, continue to stir the mixed solution and heat it, set the heating temperature to 90.0°C, when the temperature of the mixed solution rises to 50-60°C, xanthan gum and welan gum will dissolve, when the temperature of the mixed solution rises to 80-90.0°C When the gelatin is dissolved, when the temperature of the mixture rises to 90°C, continue to stir for another 10 minutes to dissolve completely and form a multi-media solution. Add distilled water to the multi-media solution to 800mL, and the sum of the concentrations of the above three media is 18.0g / L, that is, each 1L solution contains a total of 18g of xanthan gum, welan gum and gelatin to obtain a poly...

Embodiment 3

[0029] A method for stabilizing a liquid photosynthetic bacteria preparation of the present invention, in a beaker equipped with about 350mL of distilled water, in a room temperature environment, under magnetic stirring, add 12.00g NaCl and 4.0g carrageenan successively to form a mixed solution, continue to stir the mixed solution and Heating, set the heating temperature to 80.0°C, and continue stirring for 10 minutes after heating up to 80.0°C, then add distilled water to the 400mL scale to obtain a 10.0g / L carrageenan solution, wherein the NaCl content is 3.0% (w / v).

[0030] Take 360mL of the prepared 10.0g / L carrageenan solution, transfer it to a 2L blue cap bottle, sterilize it in an autoclave at 121°C for 30 minutes, cool it to about 42°C, and put it on a sterile ultra-clean workbench by passing With magnetic stirring, slowly add the bacterial suspension of purple sulfur bacteria Maichromatium gracile (Maichromatium gracile) YL28 (bacterial suspension biomass OD 660 4.53...

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Abstract

The invention discloses a method for stabilizing a liquid photosynthetic bacterium living bacterium preparation. A nontoxic, harmless and residual-free polymeric organic medium difficultly utilized byphotosynthetic bacteria is added into a photosynthetic bacterium suspension to prepare the photosynthetic bacterium liquid living bacterium preparation, and the concentration of the polymeric organicmedium is 3.0 to 6.0 g / L. The preparation can be stored in a 4-30 DEG C dark environment for at least 12 months, the appearance color of the liquid preparation has no obvious change, the preparationhas uniform properties, has no obvious liquid preparation bacterium sedimentation stratification phenomenon, and still has high biological activity, and the ability to remove nitrite nitrogen is maintained at 80% or above. The stored living bacterium preparation is miscible with water, and can be easily dispersed in water without obvious bacterial agglomerates. The preparation method of the preparation is disclosed in details, and a new use of xanthan gum and other media in the production of photosynthetic bacterium products is also developed. The method has the advantages of simplicity in operation, low cost, and easiness in large-scale operation.

Description

technical field [0001] The invention relates to the technical field of microbiology, in particular to a method for improving the storage stability of photosynthetic bacteria preparations in the production process of photosynthetic bacteria preparations. Background technique [0002] At present, anoxygenic photosynthetic bacteria ((Anoxygenic phototrophic bacteria, APB) is a general term for a class of prokaryotic microorganisms that use a variety of small molecular organic or inorganic substances as electron donors to perform oxygen-free photosynthesis under anaerobic conditions. It is called "photosynthetic bacteria (PSB)". Existing studies have shown that there are more than 80 genera and 200 species of photosynthetic bacteria, which are widely distributed in natural environments such as soil, rivers, lakes, and oceans, and play a role in the material cycle and energy cycle in nature. important role; its metabolic mode is flexible and diverse, and can carry out photoautotr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 杨素萍赵春贵张晓波周广静
Owner HUAQIAO UNIVERSITY
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