A molecular marker for esophageal cancer and uses thereof

A molecular marker, esophageal cancer technology, applied in the field of tumor molecular biology, can solve the problems of unacceptable, easy to miss diagnosis, and suffering for patients, so as to improve the effect of cancer treatment, improve the quality of life, and assist clinical treatment.

Active Publication Date: 2018-12-18
AFFILIATED HOSPITAL OF JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of contrast, X-ray barium meal contrast is currently the most commonly used and basic imaging method for displaying esophageal cancer lesions in clinical practice. It can determine the upper and lower ranges of the tumor. However, this method is prone to missed diagnosis in the early detection of esophageal cancer.
The esophageal net method is simple, practical, and highly accurate for the detection of exfoliated cells. It is the primary method for census in areas with a high incidence of esophageal cancer in my country. However, this method is more painful for patients and difficult to be widely used.
Endoscopic examination can directly observe

Method used

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  • A molecular marker for esophageal cancer and uses thereof
  • A molecular marker for esophageal cancer and uses thereof
  • A molecular marker for esophageal cancer and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] This embodiment provides a method for detecting the expression level of FXR1 gene in esophageal cancer tissue (Tumor) and paracancerous tissue (Normal), specifically comprising the following steps:

[0061] 1. Sample collection

[0062] Esophageal cancer tissues and adjacent normal tissues of esophageal cancer were collected from 115 patients with esophageal cancer. All tissue samples were from first-time patients, and were diagnosed as esophageal squamous cell carcinoma by pathologists. Esophageal cancer tissue and adjacent normal tissue specimens were obtained from the tumor resource bank of Sun Yat-sen University Cancer Center. Fresh specimens were quick-frozen in liquid nitrogen and then stored in a -80-degree refrigerator.

[0063] 2. Extract tissue RNA

[0064] (1) Take 50 mg of tumor tissue and 50 mg of normal tissue adjacent to the tumor, wash them with PBS (pH 7.4), add 1 ml Trizol, transfer to a 1.5 ml EP tube, mix well, and let stand for 5 minutes;

[0065]...

Embodiment 2

[0090] This embodiment provides a method for statistical analysis of the clinical characteristics of patients with esophageal cancer, specifically including the following:

[0091] 1. Sample information collection

[0092] Taking 115 patients diagnosed with esophageal squamous cell carcinoma in Example 1 as the experimental subjects, the hospitalization time of the patients was between 2004 and 2007, the age range was from 34 to 80 years old, and the average age was 56 years old. The clinical data and follow-up data of the patients come from the patient's inpatient medical records, outpatient follow-up records and telephone follow-up records. The gender, age, tumor location, local invasion (T stage), lymph node metastasis (N stage), distant metastasis (M stage) and pathological grade information of 115 patients were collected.

[0093] 2. SPSS 16.0 software (SPSS Inc, Chicago, IL, USA) was used for data statistics and analysis. P Figure 2A and Figure 2B ); Cox regression m...

Embodiment 3

[0102] This example provides a method for constructing a cell line that stably knocks down the FXR1 gene. The cell line is specifically the KYSE180 human esophageal cancer cell line. The vectors pHBLV-U6-Scramble-ZsGreen-Puro, pSPAX2, and pMD2G used in the construction process were all Acquired by Hanbio, the construction method includes the following steps:

[0103] 1. Design and synthesize shRNA

[0104] Taking four transcripts of FXR1 gene (NM_001013438.2, NM_001013439.2, NM_001363882.1, NM_005087.3) as molecular targets, an shRNA sequence capable of simultaneously targeting four mRNAs was designed. First, design siRNA targets, siRNA targets include siRNA1, siRNA2, siRNA3 and siRNA4, the nucleotide sequence of siRNA1 is shown in SEQ ID NO.5, the nucleotide sequence of siRNA2 is shown in SEQ ID NO.6, siRNA3 The nucleotide sequence of siRNA4 is shown in SEQ ID NO.7, and the nucleotide sequence of siRNA4 is shown in SEQ ID NO.8. Obtain shRNA1, shRNA2, shRNA3 and shRNA4 accor...

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Abstract

A molecular marker for esophageal cancer is disclosed and is an FXR1 gene and/or an expression product of the FXR1 gene. The FXR1 gene is significantly highly expressed in esophageal cancer tissue, and can be adopted as an esophageal cancer molecular marker for esophageal cancer diagnosis. The highly expressed FXR1 gene is closely related to poor prognosis and distant metastasis of patients with esophageal cancer so that the FXR1 gene can be adopted as a prognosis marker for esophageal cancer and can provide effective information for esophageal cancer distant metastasis, prognosis evaluation and treatment effect monitoring. An expression inhibitor of the FXR1 gene is disclosed by the invention and includes siRNA and/or shRNA, has good jamming effects when being used for suppressing FXR1 gene expression and has a potential for application in clinical genetic treatment. The invention further discloses a cell strain capable of stable knock-down of the FXR1 gene. The cell strain is an esophageal cancer cell capable of stable low expression of the FXR1 gene, thus facilitating further research of functions of the FXR1 gene.

Description

technical field [0001] The invention relates to the field of tumor molecular biology, in particular to a molecular marker and application of esophageal cancer, a primer set for diagnosing esophageal cancer, an expression inhibitor of FXR1 gene and a cell line stably knocking down FXR1 gene. Background technique [0002] Esophageal cancer (Esophageal carcinoma, EC) is one of the most common malignant tumors in humans, and more than 400,000 people die of esophageal cancer every year in the world. China is a country with a high incidence of esophageal cancer and one of the countries with the highest mortality rate. The number of deaths from esophageal cancer accounts for almost half of the total number of deaths from esophageal cancer in the world. According to the latest cancer epidemiological data, the incidence of esophageal cancer in my country ranks third among all kinds of malignant tumors in men, fifth in women, and fourth in both men and women in terms of mortality. Du...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11C12N15/113C12N5/10C12N15/867A61K31/7105A61P35/00
CPCA61K31/7105A61P35/00C07K14/47C12N15/113C12N15/86C12N2310/14C12N2740/15043C12Q1/6886C12Q2600/118C12Q2600/158
Inventor 葛晓松刘晓媛刘芬王晓莉高翔茆勇谢芬周少丹
Owner AFFILIATED HOSPITAL OF JIANGNAN UNIV
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