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Comb shell viscera polysaccharide, extraction method and application thereof, and drug composition thereof

A technology of shrimp scallop and extraction method, which can be applied in the directions of drug combination, pharmaceutical formula, medical preparation containing active ingredients, etc., and can solve the problems of increased atherosclerosis, severe bleeding and the like

Active Publication Date: 2018-12-21
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heparin and warfarin are currently widely used anticoagulant drugs in clinical practice, but there are still many limitations, such as the possibility of causing severe bleeding, increasing the risk of atherosclerosis and osteoporosis, etc.

Method used

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  • Comb shell viscera polysaccharide, extraction method and application thereof, and drug composition thereof
  • Comb shell viscera polysaccharide, extraction method and application thereof, and drug composition thereof
  • Comb shell viscera polysaccharide, extraction method and application thereof, and drug composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Ezo scallop viscera is freeze-dried, crushed into powder, and mixed with absolute ethanol and n-hexane at a volume ratio of 1:2 to make a solution. The mass of scallop viscera dry powder is mixed with the mixed solution at a ratio of 1:15, stirred for 1 hour, and allowed to stand Overnight, after fat removal, the upper layer of organic reagents was removed and air-dried.

[0038] Add 1200mL cysteine-EDTA disodium (0.05mol / L) solution and 4800mL K to the air-dried 1500g sample 2 HPO 4 (pH=8; 0.05mol / L) buffer solution, and then add 0.5% (m / m) trypsin (≥250N.F.U / mg) relative to the sample mass to the sample, and then add it after 4 hours of enzymatic hydrolysis in a water bath at 37°C 0.5% (m / m) of papain (≥2000U / mg) relative to the sample mass, hydrolyzed in a water bath at 65°C for 3 hours to prepare a mixed solution. The mixed solution was cooled to room temperature, centrifuged at a rotation speed of 4000r / min, for 10min, at a temperature of 4°C, and the supernatant...

Embodiment 2

[0051] This example is used to evaluate the anticoagulant effect of the visceral polysaccharide SVP2-2 of the scallop prepared in Example 1.

[0052] The specific experimental method is as follows:

[0053] Fibrinogen, thrombin and sample SVP2-2 were all dissolved in 0.05M Tris buffer (adjusted pH to 7.2 with hydrochloric acid), which contained 0.12mM NaCl. Mix 0.1% fibrinogen solution (140 μL) and 40 μL of different concentrations (such as image 3 Shown) sample solution was added to the plate well, mixed, and the absorbance was measured as the sample blank value. Then, 10 μL of thrombin solution (12 IU / mL) was added to the wells to start the thrombus precipitation reaction. After reacting for 10 minutes, measure the absorbance of the sample again. 40μL Tris-HCl buffer solution (pH 7.2, 0.05M) was used instead of the sample solution as the control blank group and the control group. Heparin was used as a positive control group. Calculate the inhibition rate formula: inhib...

Embodiment 3

[0065] Ezo scallop viscera is freeze-dried, crushed into powder, and mixed with absolute ethanol and n-hexane at a volume ratio of 1:2 to make a solution. The mass of scallop viscera dry powder is mixed with the mixed solution at a ratio of 1:15, stirred for 1 hour, and allowed to stand Overnight, after fat removal, the upper layer of organic reagents was removed and air-dried.

[0066] Add 800mL cysteine-EDTA disodium (0.05mol / L) solution and 3200mLK to the air-dried 1000g sample 2 HPO 4 (pH=8; 0.05mol / L) buffer solution, and then add 0.5% (m / m) trypsin (≥250N.F.U / mg) relative to the sample mass to the sample, and then add it after 4 hours of enzymatic hydrolysis in a water bath at 37°C 0.5% (m / m) of papain (≥2000U / mg) relative to the sample mass, hydrolyzed in a water bath at 65°C for 3 hours to prepare a mixed solution. The mixed solution was cooled to room temperature, centrifuged at 4000r / min for 10min at 4°C, and the supernatant was taken. To the supernatant, 1600 mL ...

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Abstract

The invention relates to comb shell viscera polysaccharide, an extraction method and application thereof, and a drug composition. The main chain of the comb shell viscera polysaccharide is -6)Manp(1-3)Galp(1-, wherein -SO4 exists in C-4 of Man (1- and / or C-4 site of -3Gal(1-. The polysaccharide has the effect of inhibiting thrombin to catalyze the activity of thrombin fibrinogen, can prolong activated partial thromboplastin time (APTT) and thrombin time (TT), and has no obvious influence on the prothrombin time (PT).

Description

technical field [0001] The present invention generally relates to the technical field of active polysaccharides, and in particular relates to visceral polysaccharides of scallops, extraction methods and applications thereof, and pharmaceutical compositions. Background technique [0002] Patinopecten yessoensis is mainly produced in the coastal areas of Liaoning Province and Shandong Province in China. As the scallop processing industry continues to grow, so does the amount of by-products generated from processing. Scallop viscera is a by-product of scallop processing, including the digestive tract, digestive glands, gonads, kidneys and circulatory system of scallops, accounting for about 30% of the total weight of scallops after shelling. At present, these scallop viscera are usually discarded, which not only causes environmental pollution, but also wastes biological resources. Therefore, the development and utilization of scallop viscera is of great significance. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P7/02
CPCA61K31/715A61P7/02C08B37/0003C08B37/006
Inventor 宋爽王立龙于奇艾春青温成荣王琳琳曹春阳李凌霄韩非
Owner DALIAN POLYTECHNIC UNIVERSITY
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