Transwell detection device and method based on terahertz attenuated total reflection technology

Abstract

The invention relates to a transwell detection device and method based on a terahertz attenuated total reflection technology. The transwell detection device and method can be used for conducting quickunmarked quantitative detection on cells on the lower layer of a transwell experimental cavity. The transwell detection device comprises a terahertz signal generation unit, a total reflection prism detection unit, a terahertz signal receiving unit, a time delay control system and a signal processing unit. The terahertz signal generation unit comprises a femtosecond laser light source, a laser beam splitter, a super-hemisphere Si-lens I and a photoconductive antenna I. The total reflection prism detection unit comprises a transwell cavity and a total reflection prism from top to bottom, a permeability porous thin film is arranged at the bottom of the transwell cavity, a unicellular layer is attached to the position under the porous thin film, and the unicellular layer makes close contact with the total reflection prism. The terahertz signal receiving unit comprises a photoconductive antenna II. The time delay control system comprises a time delay device.

Description

technical field [0001] The invention belongs to the technical field of terahertz detection, and relates to a transwell detection device and method based on terahertz attenuation total reflection technology. Background technique [0002] Cell and tissue culture technology is an important part of the field of basic life sciences. The use of filter membrane as a support medium for cell culture originated from Grobstein's (Natrue, 172:860-872; 1953) related research on metanephric filter induction. Obviously, compared with solid cell culture dishes and culture flasks, the permeable support interface is beneficial for cells to secrete and absorb molecules from their basal and top surfaces, and can perform metabolism more naturally and closer to in vivo conditions. Therefore, the cup-shaped device with a permeable membrane bottom layer, that is, the transwell chamber, plays an irreplaceable and important role in the study of cell interaction, migration and invasion. [0003] Int...

AI Technical Summary

Problems solved by technology

However, the terahertz spectroscopy detection of living cells is often restricted by the strong absorption interference of the liquid phase environment and the mismatch between the terahertz detection wavelength and the thickness of the single cell layer, which cannot be accurately evaluated quantitatively and qualitatively.

The specific performance is that the terahertz time-domain spectroscopy system based on the transmission mode is limited by the strong absorption of the culture medium solution, and cannot accurately obtain the single-cell layer signal in the Transwell chamber in the liquid phase solution, let alone evaluate the cell density distribution.

The metamaterial enhancement technology based on the resonant ring needs to make the cells grow and adhere to the surface of the resonant ring, and wipe off the extracellular water of the cells for detection, which cannot meet the high-throughput detection requirements of living cells

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