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Time-resolved fluorescence immunochromatographic test strip for detecting quinclorac, and preparation method and application thereof

A time-resolved fluorescence and immunochromatographic test paper technology, applied in analytical materials, biological testing, fluorescence/phosphorescence, etc., can solve problems such as inability to conduct large-scale on-site testing, affecting tobacco yield and quality, and poor timeliness, and achieving the detection time. Short, good shape, low cost effect

Active Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Quinclorac will cause obvious phytotoxicity to tobacco production, especially in rice and tobacco rotation areas, the accumulation of quinclorac will seriously affect the yield and quality of tobacco
The instrument method has the advantages of high detection sensitivity and accurate qualitative and quantitative results, but the pretreatment of the test sample is cumbersome and time-consuming, and the sample needs to be further purified. At the same time, the instrument detection method requires expensive large-scale instruments and equipment, and professional detection technicians are equipped to operate and management, unable to conduct large-scale on-site testing, poor timeliness, and difficult to promote at the grassroots level

Method used

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  • Time-resolved fluorescence immunochromatographic test strip for detecting quinclorac, and preparation method and application thereof
  • Time-resolved fluorescence immunochromatographic test strip for detecting quinclorac, and preparation method and application thereof
  • Time-resolved fluorescence immunochromatographic test strip for detecting quinclorac, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 The composition of the time-resolved fluorescent immunochromatographic test strip for detecting quinclorac

[0041] 1. Test strips

[0042] see figure 1 : The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0043]The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[0044]...

Embodiment 2

[0046] Example 2 Preparation of time-resolved fluorescent immunochromatographic test strips for detecting quinclorac

[0047] The preparation method of the time-resolved fluorescent immunochromatographic test strip for detecting quinclorac mainly comprises the following steps:

[0048] 1) Preparation of the conjugate release pad: label the quinclorac monoclonal antibody with commercially available fluorescent microspheres and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and vacuum freeze prepared after drying;

[0049] 2) Preparation of nitrocellulose membrane: Spray the quinclorac hapten-carrier protein conjugate on the detection area of ​​the nitrocellulose membrane to make the detection area; spray goat anti-mouse anti-antibody onto the nitrocellulose The range of the quality control area on the membrane is made into a quality control area;

[0050] 3) Assembly and cutting: Lay the sample absorption pad, the conjugate rel...

Embodiment 3

[0103] Example 3 Application of time-resolved fluorescent immunochromatographic test strips for detecting quinclorac

[0104] 1. Sample pretreatment

[0105] Weigh 1.0±0.05 g of the sieved soil sample into a 15 mL polystyrene centrifuge tube, add 5 mL of deionized water, vortex for 3 min, and centrifuge at room temperature (20-25°C) at 3000 rpm for 5 min; take 500 μL Put the supernatant into a 2 mL polystyrene centrifuge tube, add 500 μL deionized water to dilute, mix well, and test.

[0106] 2. Test with test strips

[0107] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be tested through the touch screen. item, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test ...

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Abstract

The invention discloses a time-resolved fluorescence immunochromatographic test strip for detecting quinclorac, and a preparation method and an application thereof. The test strip comprises a bottom plate, and a sample absorption pad, a conjugate release pad, a nitrocellulose membrane and a water absorption pad which are sequentially lapped and pasted on the bottom plate; the conjugate release padis buried with a fluorescent microsphere-labeled quinclorac monoclonal antibody; a detection area and a quality control area are fixed on the nitrocellulose membrane; the detection area is coated with a quinclorac hapten-carrier protein conjugate; the quality control area is sprayed with a goat anti-mouse anti-antibody, wherein the quinclorac hapten obtained by the steps of enabling quinclorac tobe reacted with (1, 3-dioxanylethyl) magnesium bromide to generate propylal quinclorac, and then reacting with trifluoroacetic acid. The test strip and the detection method provided by the inventionhave the advantages of simplicity in operation, high sensitivity, high detection speed, low cost and capability of realizing rapid detection and on-site monitoring of quinclorac in a large quantity ofsamples.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting quinclorac in tobacco planting soil, tobacco and tobacco products, and a preparation method and application thereof. Background technique [0002] Quinclorac is a hormone-type quinoline carboxylic acid herbicide, which is mainly used to control monocotyledonous weeds in paddy fields, especially barnyard grass. The drug can be absorbed by germinated seeds, roots and leaves, has the characteristics of hormone herbicides, and has similar symptoms to auxin substances. Quinclorac will cause obvious phytotoxicity to tobacco production, especially in rice and tobacco rotation areas, the accumulation of quinclorac will seriously affect the yield and quality of tobacco. At present, the detection methods for quinclorac residues at home and abroad mainly include high performance liquid chromato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭樊美娟崔华鹏赵乐谢复炜余晶晶尚平平
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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