A time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin and its preparation method and application

A time-resolved fluorescence and immunochromatography test paper technology, which is applied in the fields of analytical materials, biological testing, fluorescence/phosphorescence, etc., can solve the problems of high professional requirements for operators, expensive detection costs, cumbersome steps, etc., and achieve a wide range of applications , short detection time and simple operation

Active Publication Date: 2021-07-13
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the above methods require advanced detection instruments, the detection costs are expensive, the steps are cumbersome, time-consuming, and require high professionalism of operators, they are not suitable for high-throughput rapid screening detection in grassroots enterprises and institutions.

Method used

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  • A time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin and its preparation method and application
  • A time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin and its preparation method and application
  • A time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Composition of Time-Resolved Fluorescence Immunochromatography Test Strips for Detecting Pendimethalin

[0042] 1. Test strips

[0043] see figure 1 : The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0044] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[0045] Th...

Embodiment 2

[0047] Example 2 Preparation of time-resolved fluorescent immunochromatographic test strips for detection of pendimethalin

[0048] The preparation method of the time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin mainly comprises the following steps:

[0049] 1) Preparation of the conjugate release pad: mark the pendimethalin monoclonal antibody with commercially available fluorescent microspheres, and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and vacuum freeze prepared after drying;

[0050] 2) Preparation of nitrocellulose membrane: Spray the pendimethalin hapten-carrier protein conjugate on the detection area of ​​the nitrocellulose membrane to make the detection area; spray goat anti-mouse anti-antibody onto the nitrocellulose The range of the quality control area on the membrane is made into a quality control area;

[0051] 3) Assembling and cutting: Paste the sample absorption pad...

Embodiment 3

[0104] Example 3 Application of time-resolved fluorescent immunochromatographic test strips for detection of pendimethalin

[0105] 1. Tobacco sample pretreatment

[0106] Weigh 1.0±0.05 g crushed tobacco sample into a polystyrene centrifuge tube, add 5 mL of methanol, vortex for 3 min, centrifuge at 6000 rpm for 5 min at room temperature (20-25 °C), pipette 25 μL of supernatant and Mix with 475 μL deionized water and wait for detection.

[0107] 2. Test with test strips

[0108] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be Check the items, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test results on the display screen of the instrument.

[0109] 3. Analysi...

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Abstract

A time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin, a preparation method and application thereof. The test strip includes a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane, and an absorbent pad. The conjugate release pad is embedded with pendimethalin monoclonal antibody labeled with fluorescent microspheres, and fixed on the nitrocellulose membrane. There are detection area and quality control area. The detection area is sprayed with pendimethalin hapten-carrier protein conjugate, and the quality control area is sprayed with goat anti-mouse anti-antibody. The pendimethalin hapten is composed of N‑(1‑B N-(1-ethylpropyl)-3,4-dimethylaniline reacts with ethyl 4-bromobutyrate to generate alkylated N-(1-ethylpropyl)-3,4-dimethylaniline, which is then reacted with concentrated nitric acid Generate nitroalkylated N‑(1‑ethylpropyl)‑3,4‑dimethylaniline, and then react with KOH to get it. The test strip and detection method provided by the invention have the advantages of simple operation, high sensitivity, fast detection speed and low cost.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting pendimethalin in tobacco and tobacco products, a preparation method and application thereof. Background technique [0002] Pendimethalin belongs to dinitroaniline herbicides, and its mechanism of action is to inhibit meristem cell division. The part of pendimethalin absorbed by dicotyledons is hypocotyl, and the part absorbed by monocots is sprout. Pendimethalin is suitable for corn, soybean, wheat, peanut, cotton and other dryland crops to control annual grass and broad-leaved weeds such as crabgrass, foxtail and purslane. Tobacco is mainly used for chemical bud suppression and tobacco weed control. According to the classification standard of acute toxicity of pesticides in China, pendimethalin is classified as a low toxicity compound. According to the toxic classification standar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭崔华鹏樊美娟谢复炜张晓兵余晶晶王晓瑜
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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