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Candida albicans colloidal gold detecting card and application thereof

A technology of Candida albicans and colloidal gold, applied in measuring devices, immunoassays, instruments, etc., can solve the problems of high misdiagnosis rate, dependence on human judgment, lack of measurement standards, etc., and achieve accurate results, simple methods, and rapid detection effects

Inactive Publication Date: 2018-12-25
比杭生物科技(杭州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method is separated from instrument readings, relies too much on human judgment, and lacks a quantitative measure to define candidiasis
However, some small clinics and even some family doctors in Europe and the United States will directly administer medicines according to symptoms, resulting in a high rate of misdiagnosis

Method used

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  • Candida albicans colloidal gold detecting card and application thereof
  • Candida albicans colloidal gold detecting card and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Such as figure 1 and figure 2 As shown, a colloidal gold detection card for Candida albicans comprises a test paper strip and a detection card casing, and the test paper comprises a colloidal gold film 4, a nitrocellulose film 6, a sample pad 3, an absorbent pad 9, and a PVC rubber sheet 5. The sample pad 3, colloidal gold film 4, cellulose acetate film 6 and water-absorbing pad 9 are pasted on the PVC rubber sheet 5 in sequence, the sample pad 3 and the colloidal gold film 4 are side by side, and one end of the cellulose acetate film 6 is connected to the colloidal gold film. The film 4 is slightly crossed, and the other end is slightly crossed with the water-absorbent pad 9. The test strip is cut into a strip slightly narrower than the width of the test card, and then the strip-shaped test strip is installed in the long flat shell-shaped test card shell Among them, the test card housing is provided with a sample loading window 1 and a test result display window 2. ...

Embodiment 2

[0034] The preparation method of candida albicans colloidal gold detection card is realized by the following steps:

[0035](1) Preparation of colloidal gold solution: get 1 1L Erlenmeyer flask, add ultrapure water 495mL, then add 1% chloroauric acid (HAuCl 3H O) 5mL, be mixed with 500mL0.01% chloroauric acid aqueous solution, after heating and boiling Add 5-7mL of 1% trisodium citrate (Na3C6H5O7 2H2O) solution while stirring continuously, continue stirring and heating, when the color of the solution completely turns into transparent purple red, stop heating after maintaining for 5min, replenish water to the original volume, Cool to room temperature and store at 2-8°C for later use;

[0036] (2) Antibody pretreatment: centrifuge the Eno1 antibody to be labeled at 1000r / min, 4°C for 20min, take the supernatant, dilute it with 0.01mol / LPBS to 1mg / mL, and pass through a 0.22μm filter membrane;

[0037] (3) Preparation of colloidal gold markers: Take 40mL of colloidal gold soluti...

Embodiment 3

[0045] A detection method for the colloidal gold detection card of Candida albicans. After sampling the solid object to be detected, infiltrate it with 500 μl of ultrapure water or directly absorb 3-4 drops of the suspended object to be detected, and drop it into the sample window, read the result within 5 minutes, and then judge the test result according to the test status of the test card, thus completing the sample test.

[0046] Judgment of test results: If two lines are displayed, one detection line and one quality control line, it means that the result is positive, and there is Candida albicans in the test sample; if only one line of quality control line is displayed, it means that the result is negative, and the test sample is positive. Candida albicans is not present. If only one test line is displayed, the test result is invalid.

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Abstract

The invention relates to a candida albicans colloidal gold detecting card which comprises a test strip and a detecting card shell. The test strip comprises a colloidal gold film, a nitrocellulose membrane, a sample pad, a water absorbing pad and a PVC rubber board, wherein the sample pad, the colloidal gold film, the nitrocellulose membrane and the water absorbing pad are sequentially pasted to the PVC rubber board. The sample pad and the colloidal gold film are arranged side by side, one end of the nitrocellulose membrane and the colloidal gold film slightly cross, and the other end of the nitrocellulose membrane and the water absorbing pad slightly cross. The test strip is cut into a long strip which is slightly narrower than the detecting card, then the long-strip-shaped test strip is mounted in the long-strip flat-shell-shaped detecting card shell, the detecting card shell is provided with a sample adding window and a detecting result display window, the sample pad is located at the sample adding window, and the colloidal gold film is located at the detecting result display window. The candida albicans colloidal gold detecting card can be effectively used for measuring candidaalbicans, and is simple in method, convenient and rapid, and results are accurate.

Description

technical field [0001] The invention relates to the field of candida detection, in particular to a colloidal gold detection card for candida albicans and its application. Background technique [0002] Candida is a typical opportunistic pathogen that is carried in almost everyone's body. It has the characteristics of high infection frequency and wide infection area, especially in immunodeficiency patients, organ transplant patients, diabetic patients, cancer patients, the elderly and infants. Especially women's vulvovaginal infection disease sickness rate is particularly high, and almost everyone will face more than once in one's lifetime. [0003] At present, the commonly used detection method in the hospital is leucorrhea smear, and then observed through an optical microscope. Such methods deviate from instrumental readings, rely too much on human judgment, and lack a quantitative measure to define candidiasis. However, some small clinics and even some family doctors in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/531
CPCG01N33/56961G01N33/531G01N33/54306G01N2469/00G01N2800/36
Inventor 岑宇科戴富华
Owner 比杭生物科技(杭州)有限公司
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