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Seedling method for irradiating kiwi fruit seeds through high-energy carbon ion beam

A kiwifruit and ion beam technology, applied in the field of molecular genetics and breeding, can solve the problems of low germination rate and survival rate of kiwifruit seeds, and achieve the effect of high germination number and survival number and good mutagenesis effect.

Pending Publication Date: 2019-01-04
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method for raising seedlings of kiwifruit seeds irradiated by high-energy carbon ion beams provided by the invention solves the problem of low germination rate and survival rate of kiwifruit seeds after high-energy carbon ion beam radiation mutagenesis

Method used

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  • Seedling method for irradiating kiwi fruit seeds through high-energy carbon ion beam
  • Seedling method for irradiating kiwi fruit seeds through high-energy carbon ion beam
  • Seedling method for irradiating kiwi fruit seeds through high-energy carbon ion beam

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Effect test

Embodiment 1

[0036] Put 1000 Donghong kiwifruit seeds into a circular plastic transparent container (such as figure 1 Shown), the seed is placed with a thickness of 0.4cm, and a high-energy carbon ion beam irradiation experiment is carried out. This experiment is carried out at the Institute of Modern Physics, Chinese Academy of Sciences. The cumulative radiation dose of seeds treated with high-energy carbon ion beams was 50Gy. After the irradiation is completed, prepare a square breeding tray (40cm 40cm 5cm) with a hole in the bottom in a greenhouse (temperature 15-25°C), and place a piece of newspaper on the bottom. Configure the substrate (the ratio of peat soil to sand is 6:1), cover the breeding tray, and water the watering can thoroughly. The kiwifruit seeds treated with high-energy carbon ion beams were directly drilled in seedling trays at a seeding density of 3000 seeds / m 2 , Cover the matrix with 2mm, and water it thoroughly with a fine-hole watering can. Cover the seeding tra...

Embodiment 2

[0039] Put 2000 Donghong kiwifruit seeds into a circular plastic transparent container (such as figure 1 Shown), the seed is placed with a thickness of 0.7cm, and a high-energy carbon ion beam irradiation experiment is carried out. This experiment is carried out at the Institute of Modern Physics, Chinese Academy of Sciences. The cumulative radiation dose of high-energy carbon ion beam treatment seeds is 100Gy. After the irradiation is completed, prepare a square breeding tray (40cm 40cm 5cm) with a hole in the bottom in a greenhouse (temperature 15-25°C), and place a piece of newspaper on the bottom. Configure the substrate (the ratio of peat soil to sand is 6:1), cover the breeding tray, and water the watering can thoroughly. The kiwifruit seeds treated with high-energy carbon ion beams were directly drilled in seedling trays at a seeding density of 3000 seeds / m 2 , Cover the matrix with 2mm, and water it thoroughly with a fine-hole watering can. Change the water about ev...

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Abstract

The invention relates to a seedling method for irradiating kiwi fruit seeds through high-energy carbon ion beam, and belongs to the technical field of molecular genetic breeding. The method comprisesthe following steps: firstly, laying the kiwi fruit seeds into a transparent container, and performing high-energy carbon ion beam irradiation on the kiwi fruit seeds, with the irradiation strength being 40 to 100Gy, so as to obtain irradiated kiwi fruit seeds; secondly, sowing the irradiated kiwi fruit seeds into a matrix to culture the kiwi fruit seeds, so as to obtain kiwi fruit seedlings; thirdly, transplanting the kiwi fruit seedlings. Control of the radiation dosage of the high-energy carbon ion beam can achieve the purpose of effective radiation mutation, can further reduce the influence of excessive mutation on the kiwi fruit seeds by the high-energy carbon ion beam. The method can ensure that the germination rate of the kiwi fruit seeds subjected to mutation reaches 26 to 44 percent and the transplanting survival rate is greater than 70 percent, simply and effectively keeps high germination rate and survival rate of the kiwi fruit seeds subjected to mutation, and provides basic conditions for the future research on kiwi fruit materials subjected to high-energy ion mutation and realizing of high-efficiency breeding of kiwi fruit.

Description

technical field [0001] The invention relates to the technical field of molecular genetic breeding, in particular to a method for raising seedlings of kiwifruit seeds irradiated by high-energy carbon ion beams. Background technique [0002] Kiwi fruit belongs to Actinidiaceae (Actinidiaceae) genus Actinidia Lindl. This genus has 54 species, 21 varieties, and a total of 75 taxa. Actinidia plants are naturally distributed in China as the center, from the equator in the south to the cold temperate region in the north. China is the original origin center of kiwifruit germplasm resources. [0003] Kiwi fruit, also known as fox peach, vine pear, sheep peach, and kiwi fruit, is a fruit that is fresh and tender, rich in nutrition, and delicious in flavor, and people like to eat it. In addition to organic substances such as actinine, proteolytic enzymes, tannin pectin and sugars, kiwifruit contains a variety of trace elements and 17 kinds of amino acids needed by the human body, and...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01G17/00
CPCA01H1/06A01G17/005
Inventor 杨爱红周利斌刘腾云刘淑娟李彦强胡淼骆善伟余发新
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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