maldi-tof-ms desorption ionization control method, device, computer equipment and storage medium
A MALDI-TOF-MS and desorption technology, which is applied in the field of life sciences, can solve the problems of reducing the efficiency of analysis and identification of objects to be tested, and achieve the effects of improving sample analysis efficiency, improving analysis and identification efficiency, and quickly identifying results
Active Publication Date: 2020-08-11
JINAN UNIVERSITY
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Problems solved by technology
Traditional MALDI-TOF-MS is difficult to ensure that the laser is in the effective area every time in the automatic shooting mode, which will reduce the efficiency of analysis and identification of the target object
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[0059] In order to make the purpose, technical solution and advantages of the present application clearer, the present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present application, and are not intended to limit the present application.
[0060] The MALDI-TOF-MS desorption ionization control method provided by this application can be applied to such as figure 1 shown in the application environment. The processing device is respectively connected with the MALDI-TOF-MS and the control equipment, and the control equipment is connected with the MALDI-TOF-MS. Among them, MALDI-TOF-MS includes vacuum system, vacuum rapid sample change system, optical system, ion source, time-of-flight mass analyzer and data acquisition system. Spot the sample on the target plate to form a co-crystal (including matrix), put it i...
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The application relates to a MALDI-TOF-MS desorption ionization control method, a device, computer equipment and a storage medium. The method includes the following steps:target information from an external input is acquired, an image from MALDI-TOF-MS target information according to the target information is acquired; the target image is processed, a mass spectrum of each crystallization site inthe crystallization region is obtained, an effective crystallization site in the crystallization site is found, and the signal-to-noise ratio of the sample characteristic peak of the mass spectrum ofthe effective crystallization site is a preset value; a path planning is performed on the effective crystallization site, a target path is generated, the target path is output to an external controldevice, and desorption ionization is performed by an external control device according to the target path. The method can achieve desorption ionization to the effective crystallization site every time, improve the analysis speed and analyze the identification efficiency, and avoid the waste of the ionization source.
Description
technical field [0001] This application relates to the field of life science technology, in particular to a MALDI-TOF-MS (Matrix-Assisted Laser Desorption / Ionization Time of Flight Mass Spectrometry, Matrix-Assisted Laser Desorption / Ionization Time of Flight Mass Spectrometry) ionization control method, device, computer equipment and storage medium. Background technique [0002] MALDI-TOF-MS is a kind of soft ionization biological mass spectrometry. MALDI-TOF-MS mainly includes two parts: matrix-assisted laser desorption ionization source (MALDI) and time-of-flight mass analyzer (TOF). [0003] The principle of MALDI is to irradiate the co-crystal film formed by the sample and the matrix with laser light. The matrix absorbs energy from the laser and transmits it to the biomolecules. During the ionization process, the protons are transferred to the biomolecules or protons are obtained from the biomolecules, so that the biomolecules are ionized. process. Therefore, it is a s...
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IPC IPC(8): G01N27/62
CPCG01N27/628
Inventor 李磊喻佳俊曾真陈颖代新
Owner JINAN UNIVERSITY