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Quick screening method of peanut mutant and application

A screening method, mutant technology, applied in the direction of application, horticultural methods, botanical equipment and methods, etc., to achieve the effect of shortening breeding time, saving manpower, material and financial resources, and saving ineffective labor

Inactive Publication Date: 2019-01-08
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, mutant screening (identification) using the SSR method has not been reported yet.

Method used

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  • Quick screening method of peanut mutant and application
  • Quick screening method of peanut mutant and application
  • Quick screening method of peanut mutant and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Rapid Screening and Breeding Methods of Mutant Luhua 11

[0047] 1. Irradiation treatment

[0048] The full-grained Luhua No. 11 seeds (planted and preserved by Qingdao Agricultural University) were selected and subjected to fast neutron irradiation treatment at the China Institute of Atomic Energy at a dose of 9.7Gy. No irradiation was used as a control.

[0049] 2. Prepare culture medium

[0050](1) Preparation of somatic embryo induction medium: MS was selected as the basic medium, and 5-15 mg / L 2,4-D (chlorinated phenoxyacetic acid herbicide) was added to the MS medium to form a somatic embryo induction medium.

[0051] In this example, the somatic embryo induction culture was MS+10mg / L 2,4-D+30g / L sucrose+8g / L agar, and the pH was adjusted to 5.8;

[0052] 2,4-D is a chlorinated phenoxyacetic acid herbicide, which can also be used as a plant growth regulator. It is a common auxin used to induce callus formation, and is used to induce somatic embryo formation in t...

Embodiment 2

[0077] Rapid Screening and Breeding Methods of Mutant Huayu 22

[0078] 1. Materials

[0079] The peanut variety Huayu 22 was used as the test material.

[0080] 1. Irradiation treatment

[0081] The seeds of Huayu No. 22 with full grains (planted and preserved by Qingdao Agricultural University) were selected and irradiated in a high-energy mixed particle field at the China Institute of Atomic Energy. The irradiation dose was 105Gy, and no irradiation was used as a control.

[0082] 2. Prepare culture medium

[0083] (1) Preparation of somatic embryo induction medium: MS was selected as the basic medium, and 5-15 mg / L 2,4-D was added to the MS medium to form a somatic embryo induction medium. In this example, the somatic embryo induction culture was MS+10mg / L 2,4-D+30g / L sucrose+8g / L agar, and the pH was adjusted to 5.8;

[0084] (2) Preparation of somatic embryo germination and regeneration medium: MS is selected as the basic medium, and 4-6 mg / L BAP is added to this MS ...

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Abstract

The invention discloses a quick screening method of a peanut mutant and application, and belongs to the field of peanut seed breeding. The quick screening method of the peanut mutant comprises the steps as follows: identifying genotype mutagenicity of regeneration seedlings obtained by in-vitro mutagenesis of peanuts with an SSR (simple sequence repeat) method, weeding out the regeneration seedlings without genotype mutation, and identifying the regeneration seedlings with the genotype mutation as peanut mutants. The identification method of the mutagenicity of tissue culture seedlings is simple, can effectively weed out the regeneration seedlings without the mutation, saves ineffectual work of grafting, transplanting and subsequent mutagenicity identification of the regeneration seedlingswithout the mutation, and saves manpower, material resources and financial resources. The SSR method for screening the peanut genotype mutants is combined with an offspring character screening method, so that the seed breeding time can be shortened by 2-3 years. Some mutants without obvious phenotypic variation and with quality character variation can also be identified with the SSR method.

Description

technical field [0001] The invention belongs to the field of peanut breeding, and in particular relates to a rapid screening method and application of peanut mutants. Background technique [0002] Peanut is one of the important oil crops and economic crops in my country. Breeding high-yield, high-quality, special-purpose and multi-resistant new varieties is an important breeding goal of peanut at present. However, the genetic diversity of peanut cultivars is low, and there is a lack of excellent variety resources with high resistance, high oil, salt tolerance, and high yield, so it is difficult to achieve breakthroughs by cross breeding. The application of mutation technology can promote the innovation of peanut germplasm resources and accelerate the process of variety improvement, because mutation breeding can induce new traits and new types that do not exist in nature or are extremely rare. However, in conventional mutation breeding techniques, the chimerism of mutants is...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/04
CPCA01H1/04A01H4/001A01H4/008
Inventor 王晶珊禹山林乔利仙隋炯明杨庆利李冠赵明霞姜德锋郭宝太朱虹
Owner QINGDAO AGRI UNIV
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