Quick screening method of peanut mutant and application

A screening method, mutant technology, applied in the direction of application, horticultural methods, botanical equipment and methods, etc., to achieve the effect of shortening breeding time, saving manpower, material and financial resources, and saving ineffective labor

Inactive Publication Date: 2019-01-08
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, mutant screening (identification) us

Method used

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  • Quick screening method of peanut mutant and application
  • Quick screening method of peanut mutant and application
  • Quick screening method of peanut mutant and application

Examples

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Example Embodiment

[0045] Example 1

[0046] Rapid screening and breeding method of Luhua 11 mutant

[0047] 1. Irradiation treatment

[0048] The full-grained Luhua No. 11 seeds (planted and preserved by Qingdao Agricultural University) were selected for fast neutron irradiation treatment at the China Institute of Atomic Energy, the irradiation dose was 9.7 Gy, and no irradiation was used as a control.

[0049] 2. Prepare the culture medium

[0050] (1) Preparation of somatic embryo induction medium: select MS as the basic medium, and add 5-15mg / L 2,4-D (chlorophenoxyacetic acid herbicide) to this MS medium to form somatic embryo induction medium.

[0051] In this embodiment, the somatic embryo induction culture is MS+10mg / L 2,4-D+30g / L sucrose+8g / L agar, and the pH is adjusted to 5.8;

[0052] 2,4-D is a chlorinated phenoxyacetic acid herbicide, which can also be used for plant growth regulators. It is a commonly used auxin for inducing callus formation and is used to induce somatic embryo formation in th...

Example Embodiment

[0076] Example 2

[0077] Rapid Screening and Breeding Methods of Huayu 22 Mutant

[0078] 1. Material

[0079] The peanut variety Huayu 22 was used as the test material.

[0080] 1. Irradiation treatment

[0081] The full-grained Huayu No. 22 seeds (planted and preserved by Qingdao Agricultural University) were selected for high-energy mixed particle field irradiation treatment at the China Institute of Atomic Energy, with an irradiation dose of 105 Gy, and no irradiation was used as a control.

[0082] 2. Prepare the culture medium

[0083] (1) Preparation of somatic embryo induction medium: select MS as the basic medium, and add 5-15 mg / L 2,4-D to this MS medium to form somatic embryo induction medium. In this embodiment, the somatic embryo induction culture is MS+10mg / L 2,4-D+30g / L sucrose+8g / L agar, and the pH is adjusted to 5.8;

[0084] (2) Preparation of the somatic embryo germination and regeneration medium: MS is selected as the basic medium, and 4-6 mg / L BAP is added to this MS...

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Abstract

The invention discloses a quick screening method of a peanut mutant and application, and belongs to the field of peanut seed breeding. The quick screening method of the peanut mutant comprises the steps as follows: identifying genotype mutagenicity of regeneration seedlings obtained by in-vitro mutagenesis of peanuts with an SSR (simple sequence repeat) method, weeding out the regeneration seedlings without genotype mutation, and identifying the regeneration seedlings with the genotype mutation as peanut mutants. The identification method of the mutagenicity of tissue culture seedlings is simple, can effectively weed out the regeneration seedlings without the mutation, saves ineffectual work of grafting, transplanting and subsequent mutagenicity identification of the regeneration seedlingswithout the mutation, and saves manpower, material resources and financial resources. The SSR method for screening the peanut genotype mutants is combined with an offspring character screening method, so that the seed breeding time can be shortened by 2-3 years. Some mutants without obvious phenotypic variation and with quality character variation can also be identified with the SSR method.

Description

technical field [0001] The invention belongs to the field of peanut breeding, and in particular relates to a rapid screening method and application of peanut mutants. Background technique [0002] Peanut is one of the important oil crops and economic crops in my country. Breeding high-yield, high-quality, special-purpose and multi-resistant new varieties is an important breeding goal of peanut at present. However, the genetic diversity of peanut cultivars is low, and there is a lack of excellent variety resources with high resistance, high oil, salt tolerance, and high yield, so it is difficult to achieve breakthroughs by cross breeding. The application of mutation technology can promote the innovation of peanut germplasm resources and accelerate the process of variety improvement, because mutation breeding can induce new traits and new types that do not exist in nature or are extremely rare. However, in conventional mutation breeding techniques, the chimerism of mutants is...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/04
CPCA01H1/04A01H4/001A01H4/008
Inventor 王晶珊禹山林乔利仙隋炯明杨庆利李冠赵明霞姜德锋郭宝太朱虹
Owner QINGDAO AGRI UNIV
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