Quick screening method of peanut mutant and application
A screening method, mutant technology, applied in the direction of application, horticultural methods, botanical equipment and methods, etc., to achieve the effect of shortening breeding time, saving manpower, material and financial resources, and saving ineffective labor
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Embodiment 1
[0046] Rapid Screening and Breeding Methods of Mutant Luhua 11
[0047] 1. Irradiation treatment
[0048] The full-grained Luhua No. 11 seeds (planted and preserved by Qingdao Agricultural University) were selected and subjected to fast neutron irradiation treatment at the China Institute of Atomic Energy at a dose of 9.7Gy. No irradiation was used as a control.
[0049] 2. Prepare culture medium
[0050](1) Preparation of somatic embryo induction medium: MS was selected as the basic medium, and 5-15 mg / L 2,4-D (chlorinated phenoxyacetic acid herbicide) was added to the MS medium to form a somatic embryo induction medium.
[0051] In this example, the somatic embryo induction culture was MS+10mg / L 2,4-D+30g / L sucrose+8g / L agar, and the pH was adjusted to 5.8;
[0052] 2,4-D is a chlorinated phenoxyacetic acid herbicide, which can also be used as a plant growth regulator. It is a common auxin used to induce callus formation, and is used to induce somatic embryo formation in t...
Embodiment 2
[0077] Rapid Screening and Breeding Methods of Mutant Huayu 22
[0078] 1. Materials
[0079] The peanut variety Huayu 22 was used as the test material.
[0080] 1. Irradiation treatment
[0081] The seeds of Huayu No. 22 with full grains (planted and preserved by Qingdao Agricultural University) were selected and irradiated in a high-energy mixed particle field at the China Institute of Atomic Energy. The irradiation dose was 105Gy, and no irradiation was used as a control.
[0082] 2. Prepare culture medium
[0083] (1) Preparation of somatic embryo induction medium: MS was selected as the basic medium, and 5-15 mg / L 2,4-D was added to the MS medium to form a somatic embryo induction medium. In this example, the somatic embryo induction culture was MS+10mg / L 2,4-D+30g / L sucrose+8g / L agar, and the pH was adjusted to 5.8;
[0084] (2) Preparation of somatic embryo germination and regeneration medium: MS is selected as the basic medium, and 4-6 mg / L BAP is added to this MS ...
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