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A kind of high-efficiency dilution powder of pig fresh essence stored at 4 ℃ and preparation method and application

A technology of pig fresh semen and pig semen, which is applied in the field of livestock breeding, can solve problems such as disease risks, and achieve the effects of overcoming differences, low prices, and enriching preservation techniques

Active Publication Date: 2021-04-30
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, searching for research data on the storage of pig semen at 4°C shows that most cryoprotectants contain egg yolk
Egg yolk is animal-derived protein, which is easy to introduce certain pathogens into semen, and there is a certain risk of disease in production and application

Method used

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  • A kind of high-efficiency dilution powder of pig fresh essence stored at 4 ℃ and preparation method and application
  • A kind of high-efficiency dilution powder of pig fresh essence stored at 4 ℃ and preparation method and application
  • A kind of high-efficiency dilution powder of pig fresh essence stored at 4 ℃ and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Diluent preparation:

[0033] Accurately weigh the following medicines according to the above parts by weight: glucose 37g, sodium edetate 1.25g, sodium citrate 6g, sodium bicarbonate 1.25g, potassium chloride 0.75g, penicillin 1.4g, streptomycin 1.4g, Cysteine ​​0.3g, inositol 0.5g, fructose 0.3g, soybean lecithin 0.4g. The above drugs were accurately weighed into a 1000 mL beaker, then 1000 mL of double distilled water was added to the beaker, and finally dissolved and equilibrated by heating in a water bath at 37 °C for 1.5 h before use.

Embodiment 2

[0035] Pig semen dilution, packaging and storage:

[0036] After the pig semen is collected, place it at room temperature for a while to lower the temperature of the semen to about 37°C, and dilute it with the diluent of the present invention according to the original precision, so that the diluted semen density is about 0.5 billion / mL. After dilution, divide the semen into collection bottles of 80 mL each. After subpackaging, the sperm collection bottle was slowly cooled at room temperature for 30-60 minutes, and finally stored in a refrigerator at 4 °C, and the sperm collection bottle was turned every 12 hours to avoid the suspended sperm state.

Embodiment 3

[0038] Sperm preservation quality testing:

[0039] Sperm motility, vitality and other quality testing, fully shake the semen in the collection bottle to make the sperm in the bottle evenly distributed, take 10 uL of semen in a special sperm counting board, cover with a cover glass, and place at 37°C 10 fields of view were randomly selected for analysis after 2 minutes of incubation. The quality of sperm is tested once a day, and when the sperm motility rate is lower than 0.6, the storage is abandoned, so as to obtain the effective storage days of sperm.

[0040] The integrity of the sperm plasma membrane is detected by the principle of hypotonic swelling.

[0041] Preparation of hypotonic solution: Accurately weigh 7.35g of sodium citrate dihydrate and 13.51g of fructose, and dissolve them completely in 1000mL of double distilled water.

[0042] Detection of sperm plasma membrane integrity:

[0043] Take 50 µL of semen and mix it in 1 mL of hypotonic solution (37°C). Afte...

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Abstract

The invention discloses a high-efficiency dilution powder of pig fresh essence stored at 4°C and a preparation method and application thereof. 4-8, sodium bicarbonate 1.2-1.4, potassium chloride 0.7-0.8, cysteine ​​0.3-0.5, inositol 0.4-0.6, penicillin 1.0-1.5, streptomycin 1.0-1.5, soybean lecithin 0.4-1.0 . The method of use is to use 1 L of diluent prepared by the dilution powder to dilute after the pig semen is collected. The diluted semen can be stably stored in a 4°C refrigerator for 11-15 days or more, and the semen quality can still meet the requirements of insemination. , the sperm motility rate is above 0.6. The content of the present invention is the results obtained through more than 300 tests over a period of 2 years. The application in pig farms proves that pig sperm can still have insemination ability after being stored in a refrigerator at 4°C for 11 days, and the effect is stable.

Description

technical field [0001] The invention belongs to the technical field of livestock reproduction, and relates to a high-efficiency dilution powder of pig fresh semen stored at 4°C, a preparation method and application thereof. Background technique [0002] With the large-scale and intensive development of my country's pig industry and the needs of joint breeding work, pig artificial insemination technology has been widely used. Semen preservation technology has received more and more attention, and has increasingly shown many advantages such as reducing the amount and cost of boar breeding, accelerating the improvement of pig breeds, and reducing the spread of diseases. For this reason, in recent years, the technology of storing semen at room temperature has been widely used in artificial insemination of pigs. Researchers at home and abroad have carried out a lot of research work on the dilution solution of pig semen storage at room temperature, and developed a dilution solutio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0226
Inventor 崔茂盛刘鑫李千军刘朝阳李奎付永利张丹唐佩娟牟玉莲
Owner 天津市农业科学院
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