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Peripheral blood inflammatory biomarker Zonulin for detecting necrotizing enterocolitis of newborn (NEC) and application thereof

A technology of enterocolitis and sexual biology, applied in the field of inflammatory biomarkers in peripheral blood of neonatal necrotizing enterocolitis, can solve the problems of Zonulin and NEC that have not yet been seen, and achieve convenient clinical application, reduce mortality, and facilitate material collection Effect

Inactive Publication Date: 2019-01-22
东莞市第五人民医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the study of NEC patients, there is no report on Zonulin and NEC.

Method used

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  • Peripheral blood inflammatory biomarker Zonulin for detecting necrotizing enterocolitis of newborn (NEC) and application thereof
  • Peripheral blood inflammatory biomarker Zonulin for detecting necrotizing enterocolitis of newborn (NEC) and application thereof
  • Peripheral blood inflammatory biomarker Zonulin for detecting necrotizing enterocolitis of newborn (NEC) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Specimen collection and processing

[0023] Case group: Selected from March 2016 to September 2017 in the Department of Neonatology of the Sixth Affiliated Hospital of Sun Yat-sen University, the Department of Neonatology of Guangdong Maternal and Child Health Hospital (including neonatal surgery), the Department of Neonatology of the Fifth People's Hospital of Dongguan, and the Child Health Care of Foshan City. Seventy-seven patients with NEC who were hospitalized in the neonatology ward of our hospital were divided into three groups according to the final diagnosis results according to the revised Bell staging criteria of NEC in "Practical Neonatology" (Fourth Edition): Suspected NEC (28 cases of NEC stage I), Diagnosed NEC (NEC stage II, 30 cases) and progressive NEC (NEC stage III, 19 cases), and suspected NEC all had symptoms of feeding intolerance (abdominal distension, gastric retention), and all cases underwent imaging examinations.

[0024] Control...

Embodiment 2

[0026] Example 2 Detection of plasma inflammatory biomarkers by ELISA

[0027] The concentration of plasma Zonulin in the acute phase and intestinal barrier function protein was detected by enzyme-linked immunosorbent assay kit (Wuhan Huamei Bioengineering Co., Ltd.), according to the operation requirements of the instructions, as follows:

[0028] 1) Basic parameters of the kit:

[0029] Zonulin ELISA detection kit (detection range: 0.625ng / ml-40ng / ml; sensitivity: 0.156ng / ml; precision: intra-assay difference CV%<8%, inter-assay difference CV%<10%; specificity: this The kit specifically detects human Zonulin and has no cross-reaction with other related proteins).

[0030] 2) Detection principle of inflammatory biomarkers: Double-antibody sandwich ELISA (Sandwich ELISA) is used, which is suitable for the detection of soluble antigens in various liquid phases such as serum, cerebrospinal fluid, and ascites. To put it simply, the known antibody is first coated on the solid ph...

Embodiment 3

[0055] Example 3 Correlation between Bell staging of NEC and intestinal barrier function protein Zonulin

[0056] For the detection of plasma levels of intestinal barrier function-related proteins, Mann-Whitney U test was used for statistics, and Spearman rank correlation test was used for correlation analysis with Bell staging. It was found that the expression of Zonulin in the case group was significantly higher than that in the control group, such as figure 2 As shown, CTRL is the control group; NEC is the case group.

[0057] The correlation analysis between Zonulin and Bell stages (stages I, II, and III) found that Zonulin was positively correlated with Bell stages, and the correlation degree was medium to high ( image 3 ).

[0058] The invention provides a kit for detecting peripheral blood of neonatal necrotizing enterocolitis, including a reagent specifically binding to Zonulin, specifically an antibody specifically binding to Zonulin. According to the method in E...

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Abstract

The invention provides a peripheral blood inflammatory biomarker Zonulin for detecting necrotizing enterocolitis of newborn (NEC), and the inflammatory biomarker Zonulin comprises the amino acid sequence as shown in SEQ ID:1. The peripheral blood inflammatory biomarker Zonulin for detecting NEC determines Zonulin is closely related to the index of peripheral blood samples of NEC, is positively correlated with Bell staging, can serve as a marker for peripheral blood detection for NEC, and provides effective evidence for prediction, diagnosis and screening of NEC. The invention also provides a kit for detecting the peripheral blood sample of a newborn including a reagent for specifically recognizing the inflammatory biomarker Zonulin. The method is simple, rapid, economical and practical.

Description

technical field [0001] The invention relates to peripheral blood inflammatory biomarkers of neonatal necrotizing enterocolitis, in particular to inflammatory biomarker Zonulin and application thereof. Background technique [0002] Intestinal flora colonization, formula feeding, and other factors synergistically lead to an excessive inflammatory reaction in the immature intestinal mucosa of premature infants, causing intestinal epithelial cell damage and even necrosis. This is necrotizing enterocolitis of newborn (NEC) basic pathophysiological features. Therefore, the intestinal inflammatory response not only plays an important role in regulating the proliferation, differentiation and migration of epithelial cells, but also plays an important role in the process of intestinal epithelial cell injury, apoptosis and necrosis. Therefore, inflammatory response is an important link in the occurrence and development of NEC, and more and more studies are currently exploring inflamma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47G01N33/68
CPCC07K14/47G01N33/6893G01N2800/065
Inventor 王德胜
Owner 东莞市第五人民医院
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