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Application of Trifolium repens extract in preparation of medicines for improving sleep

A technology for improving sleep and clover, applied in the application, drug combination, food science and other directions, can solve the problems of sedative and hypnotic effects of clover that have not been seen, and achieve the effect of low price, improving sleep effect, and prolonging sleep time.

Active Publication Date: 2019-01-29
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, there is no research report on the sedative and hypnotic effects of clover

Method used

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  • Application of Trifolium repens extract in preparation of medicines for improving sleep
  • Application of Trifolium repens extract in preparation of medicines for improving sleep
  • Application of Trifolium repens extract in preparation of medicines for improving sleep

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1, the preparation method of cloverleaf extract

[0047] Specific steps are as follows:

[0048] (1) Get clover clover, after drying, pulverize through a 100-mesh sieve to obtain clover clover powder;

[0049] (2) dissolving the trifolium clover powder in an ethanol aqueous solution with a volume concentration of 70% according to the ratio of solid to liquid ratio of 1:5g / mL, extracting twice under heat reflux, and combining the extracts;

[0050] Wherein, the temperature of the first extraction is 70°C and the time is 2h; the temperature of the second extraction is 70°C and the time is 2h; crude extract);

[0051] (3) After diluting the cloverleaf extract with water to 1.5g / mL, use the AB-8 type macroporous adsorption resin to carry out macroporous resin column chromatography, adopt successively the water eluent of 3BV, the volume concentration of 4BV of 30% Ethanol water eluent, 4BV volume concentration 70% ethanol water eluent, 4BV volume concentration 95% ethanol...

Embodiment 2

[0103] In this embodiment, a gradient test has been done on the volume concentration of the extract (ethanol aqueous solution) in the preparation method of the cloverleaf extract in Example 1, which are respectively: 10% ethanol, 30% ethanol, 50% ethanol, 70% ethanol, 90% ethanol ethanol. Except that the concentration of ethanol is different, all the other conditions are the same as in Example 1.

[0104] The results of the extraction rate, component composition and prolonged sleep time experiment of pentobarbital sodium in mice are as follows:

[0105] Table 4 Trifolium clover extract extraction rate, component content and the impact on prolonging the sleep time of pentobarbital sodium in mice

[0106]

[0107] As shown in Table 4, when 70% ethanol is used as the extraction solvent, the total flavonoids and total saponins have the highest content, and a high-dose (500mg / kg.bw) extract is used to prolong the sleep time of barbital sodium in mice , it was found that the 70...

Embodiment 3

[0109] In this example, a gradient test was carried out on the extraction temperature of the first heat reflux extraction in the step (2) of the preparation method of the cloverleaf extract in Example 1, which were respectively: 30°C, 50°C, 70°C, and 90°C. Except that the extraction temperature is different for the first time, all the other conditions are the same as in Example 1.

[0110] The results of the extraction rate, component composition and prolonged sleep time experiment of pentobarbital sodium in mice are as follows:

[0111] Table 5 Trifolium clover extract extraction rate, component content and the impact on prolonging sleep time of pentobarbital sodium in mice

[0112]

[0113] As shown in Table 5, when 70°C was used for the first extraction, the extraction rate, total flavonoids and total saponins content were all the highest, and high dose (500mg / kg.bw) extract was used to prolong barbiturate sodium in mice During the sleep time experiment, it was found th...

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Abstract

The invention discloses application of Trifolium repens extract in preparation of medicines for improving sleep. The Trifolium repens extract is obtained by using an ethanol aqueous solution for extracting and conducting refining purification. A test of the invention finds that Trifolium repens has a certain calming and sleeping effect, and accordingly provides a new use of the Trifolium repens extract in preparation of medicines for improving sleep, and lays a foundation for developing a medicine or functional food for improving sleep performance.

Description

technical field [0001] The invention relates to the technical field of extracting traditional Chinese medicines, in particular to the application of a trifolium clover extract in the preparation of sleep-improving medicines. Background technique [0002] Since ancient times, there has been a saying that "I would rather eat without meat than sleep without sleep". Good sleep plays a vital physiological role in human growth and development, metabolism, learning and memory, survival adaptation, maintenance of immune function, recovery of physical strength and energy, etc. [0003] Long-term insomnia will bring a lot of damage to the body and mind. Insomnia may cause the body's immunity to decline, thereby weakening the body's ability to resist infection and immune surveillance; increase the risk of chronic diseases such as hypertension, diabetes, cardiovascular disease, and even asthma; cause premature aging and shorten life expectancy. In addition, insomnia can also affect th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P25/20A23L33/105
CPCA61P25/20A23L33/105A61K36/48A23V2002/00A61K2236/333A61K2236/55A61K2236/51A61K2236/39A23V2200/322
Inventor 夏道宗张晓熙
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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