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Isolated culture method of hair follicle stem cells of Yangtze River delta white goat

A technology for separating and culturing stem cells, which is applied in the field of separating and culturing hair follicle stem cells of white goat (Haimen goat) in the Yangtze River Delta, and achieves the effects of vigorous vitality, good growth and division, and high positive rate.

Inactive Publication Date: 2019-02-19
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are relatively few research reports on goat hair follicle stem cells at home and abroad, and most of them focus on fine-wool sheep and cashmere goats.

Method used

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  • Isolated culture method of hair follicle stem cells of Yangtze River delta white goat
  • Isolated culture method of hair follicle stem cells of Yangtze River delta white goat
  • Isolated culture method of hair follicle stem cells of Yangtze River delta white goat

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Embodiment Construction

[0035] 1) Main reagents and medicines

[0036] DMEM / F12 (Dulbecco's Modified Eagle's Medium / Ham's Nutrient MixtureF12, 1:1) culture medium is the product of Gibco Company; fetal bovine serum (fetal bovine serum, FBS) is the product of Gibco Company; 0.25% trypsin is the product of Gibco Company; Fibroblast growth factor (basic Fibroblast Growth Factor, bFGF), epidermal growth factor (Epidermal Growth Factor, EGF), insulin-like growth factor 1 (Insulin-like Growth Factors -1, IGF-1) are products of Sigma; 100× The double antibody is the product of Sigma; the H.E staining solution is the product of Sangon Biotech; the type I mouse tail collagen is the product of Solarbio; antibody CD34: abcam; antibody CD49F: Thermo Fisher.

[0037] Culture medium: DMEM / F12 medium containing 10% fetal bovine serum, bFGF 10ng / ml, EGF 10ng / ml, IGF-1 10ng / ml, 1% double antibody, and 1% type I rat tail collagen.

[0038] 2) Experimental animals and sample collection

[0039] The samples were colle...

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Abstract

The invention provides an isolated culture method of hair follicle stem cells of Yangtze River delta white goat. By adoption of the method, an isolated cell line has a good growth and division state,and relatively pure hair follicle stem cells can be obtained and are of a typical paving stone shape; primary cells grow fast, so that aggregated growth of cells can be observed on the third day, morecell aggregates in aggregated growing can be observed on the fourth day, the cells can overgrow 60 to 70 percent of a culture dish on the fifth day, and the positive rate of hair follicle stem cell markers is up to 90 percent or above. Results of cell chromosome analysis, hair follicle stem cell marker detection, cell activity detection and the like show that a built hair follicle stem cell lineof the Yangtze River delta white goat is high in activity, and the hair follicle stem cell markers are high in positive rate; and a good practical model can be provided for researching gene expressionand passageway regulation of skin cells and growth and a mechanisms of skin appendages, in particular for researching a mutual action mechanism among main cells of the skin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for isolating and culturing hair follicle stem cells of white goats (Haimen goats) in the Yangtze River Delta. Background technique [0002] Hair follicle stem cells widely exist in the structure of skin hair follicles. They belong to adult stem cells and are primitive cells in hair follicles. They have multi-directional differentiation potential and can produce cells needed by the body through their own division and proliferation. Adult stem cells have the characteristics of a wide range of sources, easy culture and expansion, and high pluripotency. [0003] With the continuous deepening of stem cell research, hair follicle stem cell research has become a hot spot for scholars at home and abroad. Hair follicle stem cells are located in the hair follicle bulge, and are closely related to hair cycle maintenance, tissue maintenance and renewal, and skin damage rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A01N1/02
CPCA01N1/0221C12N5/0666C12N2509/00
Inventor 李拥军王强尹修远瞿静雯
Owner YANGZHOU UNIV
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