Isolated culture method of hair follicle stem cells of Yangtze River delta white goat
A technology for separating and culturing stem cells, which is applied in the field of separating and culturing hair follicle stem cells of white goat (Haimen goat) in the Yangtze River Delta, and achieves the effects of vigorous vitality, good growth and division, and high positive rate.
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[0035] 1) Main reagents and medicines
[0036] DMEM / F12 (Dulbecco's Modified Eagle's Medium / Ham's Nutrient MixtureF12, 1:1) culture medium is the product of Gibco Company; fetal bovine serum (fetal bovine serum, FBS) is the product of Gibco Company; 0.25% trypsin is the product of Gibco Company; Fibroblast growth factor (basic Fibroblast Growth Factor, bFGF), epidermal growth factor (Epidermal Growth Factor, EGF), insulin-like growth factor 1 (Insulin-like Growth Factors -1, IGF-1) are products of Sigma; 100× The double antibody is the product of Sigma; the H.E staining solution is the product of Sangon Biotech; the type I mouse tail collagen is the product of Solarbio; antibody CD34: abcam; antibody CD49F: Thermo Fisher.
[0037] Culture medium: DMEM / F12 medium containing 10% fetal bovine serum, bFGF 10ng / ml, EGF 10ng / ml, IGF-1 10ng / ml, 1% double antibody, and 1% type I rat tail collagen.
[0038] 2) Experimental animals and sample collection
[0039] The samples were colle...
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