422results about How to "Optimize the extraction process" patented technology

The invention relates to a split heads for binding reinforcing steel bars in building engineering, in particular to a height-adjustable recyclable reinforcing steel bar split heads. The height-adjustable recyclable reinforcing steel bar split heads comprises a lifting hook, a lifting mechanism and a bracket. The bracket comprises a tray with a central through hole, and three supporting legs which are distributed in a triangular shape are hinged to the lower portion of the tray. The lifting mechanism is a lead screw lifting mechanism, a screw of the lead screw lifting mechanism is sleeved by a handle sleeve integrally and fixedly connected with a nut, the screw is inserted into the central through hole of the tray to enable the lead screw lifting mechanism to be wholly assembled on the bracket, and the lifting hook for lifting reinforcing steel bars is welded at the lower end of the screw. The height-adjustable recyclable reinforcing steel bar split heads enables reinforcing steel bar meshes to be adjusted continuously and is light and convenient to adjust. Angles of the supporting legs can be adjusted to enable the split heads to be placed at will. The height of the lifting hook and the angles can be reduced and changed at will to enable the lifting hook and the supporting legs to be conveniently pulled out of concrete. After the concrete is poured, the split heads is easy to dismount. The split heads can be manufactured by using corner waste materials in construction sites, so that the materials are convenient to obtain. The split heads saves materials and is simple to manufacture.

The present invention relates to the field of nucleic acid extraction, particularly to a nucleic acid extraction method and a nucleic acid extraction reagent for biological samples. The specific method comprises: adding a sodium hydroxide solution to a biological sample, adding a lysate, and carrying out lysis to obtain a lysis reaction liquid, wherein the lysate contains a guanidine salt, disodium edetate, a surfactant and a buffer agent; and adding an ethanol solution to the lysis reaction liquid, and achieving nucleic acid adsorption, cleaning and elution by using an instrument-free nucleic acid extraction apparatus having a silica gel membrane or a centrifugal column. According to the present invention, the sodium hydroxide is added to pre-treat and then the lysate is added to carry out lysis, such that the protein in the sample can be well dissolved, and the binding of the nucleic acid and the adsorption film is prompted; and the lysate component is optimized, such that the binding capacity of the nucleic acid molecule and the silica gel membrane under the high salt and high pH value conditions is significantly enhanced, and the good nucleic acid extraction efficiency is ensured.

The invention discloses a straw puller, and belongs to the field of farm tools. The problems of troublesomeness and low efficiency of manual straw cleaning in a special area are mainly solved. The straw puller comprises a base and a support rod upwards arranged on the base. A pedal is arranged at the lower end of the support rod. A U-shaped cavity is formed in one end of the pedal. The support rod and the pedal are hinged in the U-shaped cavity. The two front ends of the U-shaped cavity are hinged with a clamping arm respectively. The clamping arms can rotate around axes perpendicular to the pedal. A splayed limiting frame is arranged at the lower end of the support rod. The lower end of the splayed limiting frame is fixedly arranged on the base. The two clamping arms are positioned in a cavity formed by the limiting frame and the base. Springs are arranged between the clamping arms and the base. The straw puller can be used for easily pulling straws, and is time-saving, labor-saving and high in efficiency.

The invention provides a fuse mounting structure. The fuse mounting structure comprises an electric appliance box main body and a fuse holder, wherein slots are formed in the two sides of the electric appliance box main body; first bosses and second bosses are arranged inside the slots; the fuse holder comprises a fuse mounting part, clamping parts positioned on the two sides of the fuse mounting part, and two insertion parts positioned below the fuse mounting part; the insertion parts comprise sliding chutes with sealed upper ends and lower ends, and bulges protruded in the directions far away from the sliding chutes; the insertion parts of the fuse holder are inserted in the slots of the electric appliance box main body; the second bosses are positioned in the sliding chute; under the working condition, the first bosses are buckled with the bulges, and meanwhile the second bulges are not in contact with the lower ends of the sliding chutes; under the out-of-service condition, the first bosses squeeze the bulges to enable the insertion parts to be deformed towards the sliding chutes, and meanwhile the second bosses are in contact with the lower ends of the sliding chutes. The fuse mounting structure disclosed by the invention is convenient to operate and high in safety.

The invention discloses a 3D culture, passage, cryopreservation, recovery and identification method for in-vitro organoids based on small intestines of different segments of mice. The method comprisesthe following steps: (1) separating recesses of duodenum, jejunum and ileum segments of mice; (2) performing 3D culture on the recesses of the duodenum, jejunum and ileum segments of mice, and forming organoids; (3) performing passage on organoids of small intestines of mice; (4) performing cryopreservation on the organoids of small intestines of mice; (5) performing recovery on the organoids ofsmall intestines of mice; (6) preparing frozen sections of the organoids of small intestines of mice; and (7) performing immunofluorescence labeling and identification on the frozen sections of the organoids of small intestines of mice. Compared with the prior art, the method disclosed by the invention optimizes a recess extraction manner, an in-vitro culture system and a culture medium based on the recesses of the small intestines containing stem cells, and comprises the steps of complete culture, passage, cryopreservation, recovery and identification. The small intestine organoids obtained by the method are capable of performing repeated passage, and the passage organoids have character stability.

The present invention provides a bobbin fixing device capable of fixing a bobbin easily with a simple structure and also provides a bobbin removing device capable of removing a bobbin readily with a simple structure. Stoppers 35 project from the level of the outer surface of a rotating shaft 23. Sloping surface of each stopper 35 is pressed by a bobbin 10, so that the stopper is moved to an inner position P2. The bobbin 10 is attached so as to position on a positioning member 25. When the bobbin 10 is brought into contact with the positioning member 25, each support 34B upwardly moves together with a release button 32 biased by a spring 33, so that one end of each arm 34A on the associated support 34B moves upward and the other end thereof close to the associated stopper 35 moves downward. Thus, each stopper 35 outwardly moves and projects until a restricting face 35C of the stopper 35 is brought into contact with the bobbin 10. At that time, a retaining face 35B of each stopper 35 is in contact with the top surface of the bobbin 10, thus restricting the movement of the bobbin 10 in the direction (Y direction) in which the bobbin 10 is removed from the rotating shaft 23.

The invention discloses a device for realizing a reciprocating extrusion process on a one-way hydraulic press. The device is characterized in that a hollow cylindrical cavity is formed in an annular extrusion cylinder; an upper male die and a lower male die which can be in axial sliding in the hollow cylindrical cavity are respectively arranged at the top and the bottom of the hollow cylindrical cavity; a cylindrical blank is placed in the hollow cylindrical cavity; a die core inserted block is clamped and embedded into a groove in the inner cylinder wall of the annular extrusion cylinder to form an annular reduced section; the circumference of the annular extrusion cylinder is fixedly sleeved with a pre-tightening ring; a female die pressing plate is fixedly connected to the top of the pre-tightening ring and is propped against the top surface of the annular extrusion cylinder; a female die composed of the annular extrusion cylinder, the female die pressing plate, the pre-tightening ring and the die core inserted block is formed in the manner that the bottom surface of the pre-tightening ring or the top surface of the female die pressing plate is placed in a downward-concave platform on the top surface of a female die fixing plate to form an upright-placing or inverted state; an nitrogen spring in the hollow cavity of a base forms underpropping to the lower male die. According to the invention, the reciprocating extrusion technology on the one-way hydraulic press is realized in an assisting way, reciprocating extrusion deformation under different extrusion ratios can be satisfied, and the fine and equiaxed fine-grain tissues can be achieved.

A paper money binding machine is composed of a paper money input unit, a separating feeder for separating the paper money from each other and feeding them out, a delivering unit for delivering paper money one by one in their short edge direction, a recognizing unit, a collecting unit for collecting a predefined number of paper money, a moving unit for moving them in their long edge direction, a binding unit for holding the collected paper money and binding them to become bundle, a bundle discharging outlet, a branch delivering unit at downstream of recognizing unit, and a paper money discharging port for the paper money which is not bound.

The invention discloses a plastic container, a long flow member and a coating apparatus, being able to avoid the harmful condition that the flow path of the viscous liquid is closed when the plastic container body is shriveled, and to eliminate the condition that the viscous construction member adhered to the pipe when the pipe is repeatedly used so as to pollute the surrounding. The plastic container comprises a plastic container body which is provided with an opening and is filled with the viscous liquid and has flexibility so as to help the viscous liquid ejected from the opening when the container is shriveled by compression, a long flow member which extends to the inside of the plastic container body and arranged at the opening, wherein the ejection flow path at least beginning with the side surface formed when the viscous liquid is ejected is taken as the ejection flow path of the viscous liquid; and when the plastic container body is shriveled by compression, the shriveled plastic container and the long flow member can be deprecated together.

The invention relates to an assembled buckle structure which comprises a substrate (2) to be inserted in a decorative strip (1). The lower end surface of the substrate (2) is provided with a clamping part which is clamped in a mounting hole (31) of a counterpart piece (3). The assembled buckle structure is characterized in that the clamping part comprises a plurality of elastic strips (4) which extend downwards and are arranged at intervals, the elastic strips are encircled into a loop matched with the shape of the mounting hole on the counterpart piece, and the lower part at the outer side of each elastic strip is provided with clamping pins (41) which protrude outwards and gradually expand from bottom to top; and a bolt (5) is inserted from bottom to top in the space encircled by the elastic strips, and the peripheral surface of the bolt acts on the inner end surfaces of the elastic strips so that the elastic strips keep the outwards bracing trend. Because of the favorable elasticity of the elastic strips, the interference between the clamping pin size and the mounting hole is smaller, the elastic strips can be inserted in the mounting hole by only needing small inserting force, and the peripheral surface of the bolt can brace the elastic strips outwards so that the elastic strips are clamped in the mounting hole and hardly drawn out.

A mold clamping apparatus able to shorten the cycle time required for mold opening and clamping operations and enabling easy pullout of tie bars, provided with a fixed die plate for holding a fixed die, a moveable die plate for holding a moveable die plate and provided movable in mold opening/closing directions A1,A2, tie bars provided with linking parts at their free ends and supported by the moveable die plate, clamping cylinders provided at the moveable die plate and driving the moveable die plate relative to the tie bars, movement mechanisms for making the moveable die plate move in the mold opening/closing directions A1,A2 and opening and closing the dies, contacting members against which the free ends of the tie bars contact so as to position the tie bars at predetermined positions with respect to the fixed die plate, and half nuts for linking with linking parts of the tie bars positioned at the predetermined positions and linking the tie bars with the fixed die plate.

The invention discloses a settlement ring for precise self-positioning of a layered settlement monitoring system and a use method of the settlement ring. The settlement ring comprises a magnetic settlement ring, an installation position self-releasing bolt and a steel plate elastic fork reed. The settlement ring is characterized in that a movable precise self-positioning settlement ring is sleeved outside a settlement tube; a spring piece is arranged on the precise self-positioning settlement ring; the precise self-positioning settlement ring is fixed on the settlement tube by using the installation position self-releasing bolt; and at the same time, the spring piece is tied by using a special chain and an external traction steel wire. The structure of the precise self-positioning settlement ring is changed, the magnetism of the precise self-positioning settlement ring is increased, and the installation position self-releasing bolt, the external traction steel wire, the chain and the like are additionally arranged; the installation position self-releasing bolt is capable of precisely positioning the precise self-positioning settlement ring on a soil body to be measured; the external traction line can be easily pulled out from the chain; and the released steel plate elastic fork reed is large in elasticity and can be well embedded into the soil body, so that the displacement of the settlement ring is consistent with the displacement of the measured soil body.

The invention provides an electric connector for flat conductor which connects a flat conductor in such a manner that even under great force on the flat conductor, the flat conductor does not come off easily. A moveable structure is provided with clamping arms for the clamping parts at two side ends of the flat conductor to be clamped. Each clamping arm comprises an elastic arm part extending in the back direction, a clamping part protruding downwardly from the back part to have the clamping part of the conductor to be clamped. A limiting part is also arranged along the array direction of the clamping part. The housing is arranged at a position at the back and abuts to the lifting direction to complete insertion of the flat conductor. The clamping part is arranged at the position where when the flat conductor is pulled in the lower direction, the flat conductor will be clamped tight and abuts against the limiting part.

The invention discloses a socket convenient for plugging and pulling, which comprises a plug and a socket. The plug and the socket are square boxes. The inner wall of the plug is welded with a first spring, and the first spring is welded with a retainer. A circular slot is arranged in the end face of one side of the retainer near the socket. A through hole is arranged in one side of the plug nearthe socket. The plug includes two conductive sheets. The inner wall of one side of the socket near the plug is welded with two reeds. One side of the socket near the plug is connected in a sliding manner with a guide rod in a side wall penetrating manner. The advantage is that electromagnetic coils generate opposite magnetic fields to push second permanent magnets to move, hydraulic oil pushes pistons to squeeze the conductive sheets, and the conductive sheets and the reeds are closely jointed, so that poor contact caused by the separation of the conductive sheets from the reeds or the dangerous situation caused by the direct separation of the plug from the socket is prevented.

The invention discloses a full-grouting sleeve adopting eccentric connection of reinforcing steel bars and a usage method of the sleeve, belongs to the technical field of concrete buildings and can decrease the protection layer thickness, prevent concrete cracking and improve project quality. The sleeve comprises a sleeve body, wherein an upper inner cylinder and a lower inner cylinder are arranged in the sleeve body and communicated with each other; the sleeve is characterized in that the upper and lower inner cylinders are arranged eccentrically, and reinforcing steel bar limiting stop blocks are arranged at ends of inner sides of the upper and lower inner cylinders; and barbs are arranged on inner surfaces of the upper and lower inner cylinders respectively. The usage method comprises steps as follows: an exposed reinforcing steel bar of an assembled component is sleeved with the upper inner cylinder, and one end of the reinforcing steel bar reaches the position of the upper reinforcing steel bar limiting stop block; an exposed reinforcing steel bar of a to-be-assembled component is inserted into the lower inner cylinder, and one end of the reinforcing steel bar reaches the position of the lower reinforcing steel bar limiting stop block; a grouting material is injected into a grouting hole until the whole inner cylinders are filled with the grouting material; after the grouting material reaches the specified strength, strength checking is performed on a strength checking section.

The invention provides an assembly for transport which can hold cylindrical members having different lengths without performing the replacement of componentry. This assembly for transport comprises a rectangular base body (1), and divided holders (2, 3). In this case, the rectangular base body (1) has inserted holding plate sections (7, 7') which are formed along both edge sections being in parallel with each other while keeping an interval, and are formed in the direction being orthogonal to both edge sections. The divided holders are detachably fitted on the inserted holding plate sections, are arranged along both edge sections being in parallel with each other of the rectangular base body (1), and on which long-shaped cylindrical members (M1, M2) are bridged. The divided holder (2) which is arranged along one edge section of the rectangular base body, and the divided holder (3) which is arranged along the other edge section of the rectangular bas body have a standing plate section (12). In this case, the standing plate sections (12) extend in the standing direction to the rectangular base body, and hold both end sections of the cylindrical members while cooperating with each other. The standing plate section (22) of the divided holder (3) can hold the cylindrical member having a different length by changing the inserting direction to the inserted holding plate section (7').

The invention discloses an outdoor power switch cabinet convenient for maintenance, comprises a bottom plate and a cabinet body, A support base is arranged on that bottom plate, the supporting base isprovided with a groove inside, a shock absorbing pillar is fixedly installed inside the groove, the outer side of the shock absorbing pillar is provided with a spring and is fixedly connected with the bottom of the cabinet body, the inside of the cabinet body is provided with two layers of partition plates, the two ends of the partition plate are movably connected with fixed blocks on the cabinetbody, The inside of the fixing block is provided with a cavity, the inside of the cavity is movably connected with both ends of the partition plate, the top of the partition plate is provided with afixing plate, the top of the fixing plate is fixedly connected with an adjusting screw rod, the bottom of the partition plate is provided with a through groove, the through groove is movably connectedwith balls, and the balls are embedded on the support block. Through the combination of the fixing block, the fixing plate, the adjusting screw, the ball and the supporting block, the separator is convenient to be pulled out, the worker is convenient to carry out maintenance or overhaul, and the operation is simple and very convenient.

A pneumatic tire 10 in which a plurality of circumferential main grooves 11 that extend in the tire circumferential direction are formed in a tread surface portion, and a plurality of land portion rows 14 are partitioned by the circumferential main grooves 11, in which in at least one of the plurality of the land portion rows 14a circumferential auxiliary groove 15 extending in the tire circumferential direction and having a narrower width and shallower depth than those of the circumferential main grooves 11 is formed in a position avoiding the tire centerline CL, and among both side faces 15a and 15b defining the circumferential auxiliary groove 15, one side face on the tire centerline side extends along the normal direction of the tread surface portion or is inclined to the tire centerline side by 5 DEG or less with respect to the normal direction, and the other side face on the side opposite to the tire centerline side is inclined to the side opposite to the tire centerline side by 10 DEG to 30 DEG with respect to the normal direction. In accordance with the present invention, it is possible to improve the turning performance on snowy roads without decreasing the steering stability or quietness during travel on a dry road surface.

The invention discloses a slotting device for the inner wall of a spun yarn ring spinning bobbin, and belongs to the technical field of ring spun yarn equipment. The slotting device comprises a substrate, a bobbin positioning assembly, a cutter bar, a motor, a transmission mechanism and at least one cutter, wherein the substrate is provided with a sliding groove; the cutters are fixed to the cutter bar and protrude out of the cutter bar; the cutter bar is embedded in the sliding groove in a sliding manner; and the bobbin positioning assembly comprises a slotware, and the slotware is installed on the substrate and used for storing a bobbin to be processed. The motor is connected with the cutter bar through the transmission mechanism, and drives the cutter bar and the cutters to move in the longitudinal direction to cut an inner barrel of the bobbin. The slotting device is used for conducting slotting in the inner wall of the bobbin for ring spun yarns, in this way, the bobbin can be firmly attached to a spindle blade and can also be pulled out easily, and the situation that when a bobbin is pulled out, because an inner barrel of the bobbin and a spindle blade are attached too tightly, a sealing area lower than the atmosphere pressure is partially generated, and the atmosphere pressure hinders the bobbin from being pulled out is avoided.

The invention discloses a pile pulling device and method for a reinforced concrete prestressed pipe pile. The pile pulling device comprises a pile pulling device body, an anchor head, a second anchoring body and anchor cables, and the pile pulling device body further comprises a pull module used for applying pull on the anchor cables and a clamping opening used for pulling a pile body. Firstly, a layer of bonding agent is smeared on the inner wall of the bottom of the pile body; then a first anchoring body is cast at the bottom of the pile body, the anchor cables and the anchor head are put into the bottom, and the anchor head and the first anchoring body are bonded; then a casting pipe extends to the bottom of the pile body again to cast the second anchoring body on the anchor head; then the pile pulling device body is erected on the ground, and the clamping opening is arranged on the pile body in a clamping mode; and finally, after the second anchoring body and the first anchoring body are solidified and formed, vertically-upward pull is applied on the anchor cables and the clamping opening simultaneously, and the pull acts on the top and the bottom of the pile body simultaneously, so that the whole pile body is pulled out successfully, and the pile body cannot be damaged or broken due to pulling; and the pile pulling device for the reinforced concrete prestressed pipe pile further has the advantages of being simple in structure and convenient to operate.

The invention discloses a vena cava filter. The vena cava filter comprises a plurality of support rods, wherein one end of each of the support rods is gathered, and the other end of each of the support rods is radially dispersed under a releasing state; at least one part of the support rods are twisting support rods which are respectively formed by twisting a plurality of metal wires; each twisting support rod is formed by twisting two or more metal wires; the diameters of the metal wires on the same twisting support rod are the same or are different; at least one metal wire of each twisting support rod is bent at a part close to the radial end to form an anchor hook. The vena cava filter has the advantages that the strength is higher, the compliance is better, the releasing and recycling process is smooth, and the falling and dislocation risks are avoided.

The invention provides a stator potting method which comprises the following steps: (1) preparing a sealant; (2) sealing two axial ends of the stator, wherein a potting opening is reserved in one end; (3) applying the sealant to the outer side face of the axial direction of the stator; (4) coaxially inserting a sealing plug core in the middle of the stator, wherein the sealing plug core is in sealed contact with the inner side face of the stator; (5) potting into the stator from the potting opening; (6) feeding the stator into a curing furnace for performing high-temperature curing; and (7) cooling and demolding. The invention further provides a motor comprising the stator produced by the stator potting method, wherein glue is potted into the stator of the motor. The stator potting method disclosed by the invention has the advantages that the stator in easy adhesion with oil is potted by utilizing the sealant so as to form a smooth stator surface, and the cleaning difficulty of the motor is greatly reduced on premise of not influencing the motor performance.

The invention relates to a bored cast-in-place pile casing. The casing comprises a cylindrical tubular casing body, a cutting seam and a plurality of buckles, wherein a pair of lifting lugs is oppositely arranged on the two sides of one end of the casing body. The casing is characterized in that the casing body (1) is formed by rolling a steel plate with the wall thickness of 6 to 8mm; the internal diameter of the casing body is 100m more than the diameter of a pile body; the cutting seam (3) is formed on the side wall of the casing body, is a through long cut which is formed along the height direction and penetrates through the two ends of the casing body, and has the width of 5mm; a plurality of buckles (2) are respectively arranged at the upper and lower ends of the cutting seam (3) and averagely arranged between the upper and lower ends; the distance between two buckles (2) is 250 to 350mm; and each buckle (2) comprises a buckle tail (4), a buckle bolt (5), a buckle check ring (6) and a hexagonal nut (7).

A two-wheeled motor vehicle configured in such a manner that an air cleaner case is provided compactly to reduce the height of the seat and that the air cleaner case can be disposed close to the engine. A two-wheeled motor vehicle (10) is provided with a vehicle body frame (11), a fuel tank (35) which is disposed at the front of and above the vehicle body frame (11), a seat (36) which is disposed behind the fuel tank (35) and above the vehicle body frame (11), an engine (12) which is suspended below the fuel tank (35) and below the vehicle body frame (11), an air cleaner case (61), a plate-like element (70) which is disposed within the air cleaner case (61), and a maintenance lid (72) which is used when the plate-like element (70) is mounted and dismounted. The air cleaner case (61) is disposed below the fuel tank (35), the plate-like element (70) is disposed so as to be tilted rearward and upward in a side view of the vehicle, and the maintenance lid (72) is disposed below the seat (36) so as to face the seat (36).