Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method and application of total flavonoids extract of willow-leaf wintersweet

A technology of total flavonoids and extracts, which is applied in the field of preparation of total flavonoids extracts of Wintersweet willow leaves, can solve the problems of limited therapeutic effect of NAFLD, achieve relief or treatment of non-alcoholic fatty liver disease, maintain liver index, improve anti-inflammatory The effect of oxidative damage capacity

Active Publication Date: 2021-12-17
LISHUI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above drugs have limited therapeutic effect on NAFLD and can only improve some symptoms of NAFLD patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method and application of total flavonoids extract of willow-leaf wintersweet
  • A kind of preparation method and application of total flavonoids extract of willow-leaf wintersweet
  • A kind of preparation method and application of total flavonoids extract of willow-leaf wintersweet

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Preparation of total flavonoids extract of Chimerus osmanthus.

[0030] 1) Grind the new leaves of Wintersweet, pass through a 200-mesh fine sieve, add petroleum ether according to the ratio of solid to liquid (1:2 (g / mL)) according to the powder weight of Wintersweet, extract for 12 hours, and filter to remove the extract , take by weighing several grams of willow wax plum leaf powder after petroleum ether degreasing, add the ethanol solution that volume fraction is 20% according to solid-liquid ratio 1:5 (g / mL), set extraction temperature as 60 ℃, carry out reflux extraction A crude extract was obtained.

[0031] 2) After the crude extract is vacuum filtered, the filtrate is combined, concentrated under reduced pressure to 1 / 8 of the original volume, and the concentrated solution is chromatographically processed through a D101 macroporous resin column (Tianjin Haiguang). The specific method is: macroporous resin Pretreatment: The resin is soaked in 95% eth...

Embodiment 2

[0034] Example 2. Preparation of total flavonoids extract from Chimerus osmanthus.

[0035] 1) Pulverize the new leaves of the willow wintersweet, pass through a 200-mesh fine sieve, add petroleum ether according to the weight of the willow wintersweet leaf powder according to the ratio of material to liquid (1:2 (g / ml), extract for 12 hours, filter to remove the extract, Weigh a few grams of willow wax plum leaf powder after degreasing petroleum ether, add a volume fraction of 75% ethanol solution according to the solid-liquid ratio of 1:25 (g / mL), set the extraction temperature to 80 ° C, and carry out reflux extraction to obtain crude extract.

[0036] 2) The crude extract is vacuum filtered and the filtrate is combined, concentrated under reduced pressure to 1 / 10 of the original volume, and the concentrated solution is chromatographically processed through a DM-130 macroporous resin column (Tianjin Haiguang). The specific method is: Porous resin pretreatment: soak the res...

Embodiment 3

[0039] Example 3. Preparation of total flavonoids extract from Chimerus osmanthus.

[0040] 1) Pulverize the new leaves of the willow wintersweet, pass through a 200-mesh fine sieve, add petroleum ether according to the weight of the willow wintersweet leaf powder according to the ratio of material to liquid (1:2 (g / ml), extract for 12 hours, filter to remove the extract, Weigh a few grams of the powdered Wintersweet leaves after petroleum ether degreasing, add 80% ethanol solution according to the ratio of solid to liquid 1:50 (g / mL), set the extraction temperature to 90°C, and carry out reflux extraction to obtain a crude extract.

[0041] 2) After the crude extract is vacuum filtered, the filtrate is combined, concentrated under reduced pressure to 1 / 2 of the original volume, and the concentrated solution is subjected to chromatography through HPD100 macroporous resin column (Tianjin Haiguang). The specific method is: macroporous resin Pretreatment: The resin is soaked in 9...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A preparation method and application of the total flavonoids extract of Chimerus osmanthus, which belong to the field of medicine and health care products. Aiming at the technical problem of limited drug efficacy of current drugs used to treat non-alcoholic fatty liver, the invention provides a willow The preparation method of the total flavonoids extract of Chimerus chinensis comprises the following steps: after pulverizing the leaves of Wintersweet, adding 20% ​​- 80% of the volume fraction according to the ratio of solid to liquid 1: (5 - 50) (g / mL) Ethanol solution, reflux extraction at a temperature of 40-90°C to obtain a crude extract; the crude extract is vacuum filtered, and the obtained filtrate is washed with a volume fraction of 20%-80% ethanol solution after macroporous resin column chromatography The eluate was collected, concentrated and evaporated to dryness to obtain the total flavonoids extract of Chimerus flavonoids. The total flavonoid extract prepared by the invention can be used to prepare liver-protecting medicines or health products.

Description

technical field [0001] The invention belongs to the field of medicine and health care products, and in particular relates to a preparation method and application of total flavonoids extract of Chimerus osmanthus. Background technique [0002] Fatty liver is the first liver disease in developed countries. In recent years, the incidence of fatty liver in China has been increasing, and now it has become the second liver disease after viral hepatitis. Fatty liver refers to the lesion of excessive fat accumulation in liver cells caused by various reasons. It is a common pathological change of the liver, rather than an independent disease. Fatty liver disease is a serious threat to the health of Chinese people, the incidence rate is increasing, and the age of onset is getting younger. Normal human liver tissue contains a small amount of fat, such as triglycerides, phospholipids, glycolipids, and cholesterol, and its weight is about 3% to 5% of the liver weight. If too much fat ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P1/16A23L33/105A61K127/00
CPCA61P1/16A23L33/105A61K36/185A61K2236/333A61K2236/51A61K2236/55A23V2002/00A23V2200/32A23V2250/2116
Inventor 柳海燕侯景乔燕俞泓彬肖建中何小勇温慧萍潘心禾
Owner LISHUI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com