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Method for preparing total flavonoid extract of chimonanthus salicifolius and application thereof

A technology of total flavonoids and extracts, which is applied in the field of preparation of total flavonoids extracts of Chimerus chinensis, can solve the problems of limited therapeutic effect of NAFLD, achieve the effect of improving the ability of anti-oxidative damage, expanding the application field, and maintaining the liver index

Active Publication Date: 2019-03-19
LISHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above drugs have limited therapeutic effect on NAFLD and can only improve some symptoms of NAFLD patients

Method used

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  • Method for preparing total flavonoid extract of chimonanthus salicifolius and application thereof
  • Method for preparing total flavonoid extract of chimonanthus salicifolius and application thereof
  • Method for preparing total flavonoid extract of chimonanthus salicifolius and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Preparation of Chimonanthus praecox total flavonoids extract.

[0030] 1) Crush the new leaves of Chimonanthus praecox, sieving through a 200-mesh fine sieve, add petroleum ether according to the weight of the Chimonanthus praecox leaf powder according to the material-to-liquid ratio 1:2 (g / mL), extract for 12h, and filter to remove the extract , Weigh several grams of Chimonanthus praecox leaf powder after degreasing with petroleum ether, add 20% ethanol solution in volume fraction according to the material-to-liquid ratio of 1:5 (g / mL), set the extraction temperature to 60℃, and perform reflux extraction A crude extract is obtained.

[0031] 2) After vacuum filtration of the crude extract, the filtrate is combined and concentrated under reduced pressure to 1 / 8 of the original volume. The concentrated solution is subjected to chromatography through a D101 macroporous resin column (Tianjin Haiguang). The specific method is: macroporous resin Pretreatment: The res...

Embodiment 2

[0034] Example 2. Preparation of Chimonanthus praecox total flavonoids extract.

[0035] 1) Crush the new leaves of Chimonanthus praecox, pass a 200-mesh sieve, add petroleum ether according to the weight of Chimonanthus praecox leaf powder according to the material-to-liquid ratio of 1:2 (g / ml), extract for 12h, filter to remove the extract, Weigh several grams of Chimonanthus chinensis leaf powder after degreasing with petroleum ether, add 75% ethanol solution in volume fraction according to the material-to-liquid ratio of 1:25 (g / mL), set the extraction temperature to 80°C, and perform reflux extraction. Crude extract.

[0036] 2) After the crude extract is vacuum filtered, the filtrate is combined and concentrated under reduced pressure to 1 / 10 of the original volume. The concentrated solution is subjected to chromatographic processing through a DM-130 macroporous resin column (Tianjin Haiguang). The specific method is: Pretreatment of porous resin: The resin is first immersed...

Embodiment 3

[0039] Example 3. Preparation of the total flavonoids extract of Chimonanthus praecox.

[0040] 1) Crush the new leaves of Chimonanthus praecox, pass a 200-mesh sieve, add petroleum ether according to the weight of Chimonanthus praecox leaf powder according to the material-to-liquid ratio of 1:2 (g / ml), extract for 12h, filter to remove the extract, Weigh several grams of Chimonanthus praecox leaf powder after petroleum ether degreasing, add 80% ethanol solution according to the material-to-liquid ratio of 1:50 (g / mL), set the extraction temperature to 90°C, and perform reflux extraction to obtain a crude extract.

[0041] 2) After the crude extract is vacuum filtered, the filtrate is combined and concentrated under reduced pressure to 1 / 2 of the original volume. The concentrated solution is subjected to chromatography through HPD100 type macroporous resin column (Tianjin Haiguang). The specific method is: macroporous resin Pretreatment: The resin is first immersed in 95% ethanol b...

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Abstract

The invention discloses a method for preparing total flavonoid extract of chimonanthus salicifolius and application thereof and belongs to the fields of medicines and health care products. Aiming at the technical problem that the present medicine for treating non-alcoholic fatty liver is limited in efficacy, the invention provides the method for preparing total flavonoid extract of chimonanthus salicifolius. The method comprises the following steps: grinding chimonanthus salicifolius leaves, adding an ethanol solution having a volume fraction of 20-80% according to a solid-liquid ratio of 1:(5-50) (g / mL), and performing reflux extraction at a temperature of 40-90 DEG C so as to obtain crude extract; performing vacuum filtration on the crude extract, performing macroporous resin column chromatography on the obtained filtrate, eluting with the ethanol solution having the volume fraction of 20-80%, collecting the eluant, concentrating and evaporating to dryness, thereby obtaining the total flavonoid extract of chimonanthus salicifolius. The total flavonoid extract prepared by the invention can be used for preparing drugs or health products for protecting the liver.

Description

Technical field [0001] The invention belongs to the field of medicine and health care products, and specifically relates to a preparation method and application of the total flavonoids of Chimonanthus praecox. Background technique [0002] Fatty liver is the number one liver disease in developed countries. In recent years, the incidence of fatty liver in China has been increasing, and it has now become the second liver disease after viral hepatitis. Fatty liver (fatty liver) refers to the pathological changes of excessive fat accumulation in liver cells caused by various reasons. It is a common liver pathological change, rather than an independent disease. Fatty liver disease is seriously threatening the health of Chinese people, the incidence rate is increasing, and the age of onset is getting younger. Normal human liver tissue contains a small amount of fat, such as triglycerides, phospholipids, glycolipids, and cholesterol. Its weight is about 3% to 5% of the liver weight. If...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P1/16A23L33/105A61K127/00
CPCA61P1/16A23L33/105A61K36/185A61K2236/333A61K2236/51A61K2236/55A23V2002/00A23V2200/32A23V2250/2116
Inventor 柳海燕侯景乔燕俞泓彬肖建中何小勇温慧萍潘心禾
Owner LISHUI UNIV
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