Culture system for preparing endoderm cell line and method for preparing endoderm cell line

An endoderm cell and culture system technology, applied in the field of preparation of endoderm cell lines, can solve problems such as hindering the application of technology

Inactive Publication Date: 2019-03-19
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, although the research progress of transcription factor-based reprogramming to change cell fate is of great significance to the research of regenerative medicine and life sciences, overexpression of exogenous transcription factors through retrovirus integration into the genome of somatic cells There are potential safety issues, such as the use of the proto-oncogene C-Myc leading to tumor formation (Nakagawa et al., 2007), which hinder the clinical application of this technology (Hawley, 2008, Does retroviral insertional mutagenesis play a role in the gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture system for preparing endoderm cell line and method for preparing endoderm cell line
  • Culture system for preparing endoderm cell line and method for preparing endoderm cell line
  • Culture system for preparing endoderm cell line and method for preparing endoderm cell line

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0063] The donor-derived cells used in the method of producing an endoderm cell line of the present invention may be cells of various mammals. Examples of suitable mammalian cells include, but are not limited to: fibroblasts, bone marrow-derived monocytes, skeletal muscle cells, adipocytes, peripheral blood mononuclear cells, macrophages, neural stem cells, hepatocytes, keratinocytes, oral Keratinocytes, hair follicle dermal cells, gastric epithelial cells, lung epithelial cells, synoviocytes, kidney cells, skin epithelial cells or osteoblasts.

[0064] The cells from the donor can also be cells derived from different types of tissue, eg, bone marrow, skin (eg, dermis, epithelium), scalp tissue, muscle, adipose tissue, peripheral blood, skeletal muscle, or smooth muscle. Cells can also be derived from neonatal tissues including, but not limited to: umbilical cord tissue (e.g., umbilical cord, cord blood, cord blood container), amniotic membrane, placenta, or other various neon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a culture system for preparing an endoderm cell line and a method for preparing the endoderm cell line by the culture system. The culture system comprises a composition of a TGF-beta receptor antagonist, an RA receptor activator and a cAMP (cyclic adenosine monophosphate) activating agent, wherein the composition is free from exogenous transcription factors. By the aid of the culture system and the culture method, reprogramming for changing cell fate can be induced, and body cells are reprogrammed into the endoderm cell line.

Description

technical field [0001] The invention relates to a culture system for preparing endoderm cell lines by inducing the reprogramming of small chemical molecules and growth factors to change cell fate, and a method, kit and application for preparing endoderm cell lines using the culture system. Background technique [0002] All organisms rely on transcriptional machinery to express specific portions of the genome in response to changes in the environment or developmental signals, thereby performing key biological functions throughout the life cycle. Thus, the transcription process constitutes a critical step in the regulation of biological processes, and transcription factors are considered to be master switches in determining cell fate. In the past decade, the rapid development of stem cell biology has mainly benefited from the elucidation of the functions of several transcription factors, which are the main regulators of the pluripotency of induced pluripotent stem cells (iPSCs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/071C12N5/073
CPCC12N5/0603C12N5/067C12N2501/01C12N2501/115C12N2501/15C12N2501/155C12N2501/16C12N2501/405
Inventor 裴端卿刘晶曹尚涛余胜勇陈捷凯
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap