Tremella mesenterica effective protospecies and preparation method thereof
A kind of technology of golden ear and original seed, applied in the field of effective original seed of golden ear and its preparation, can solve the problems such as insufficient stability of fruiting bodies of golden ear, weak development of industrial cultivation of golden ear, poor quality of golden ear fungus, etc. Achieve the effects of low production cost, fast mycelia feeding, and simple composition
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preparation example Construction
[0020] The preparation method of the effective stock of golden ear of the present invention comprises the steps of pretreatment and inoculation and cultivation, specifically comprising:
[0021] A. Pre-processing:
[0022] 1) Select dry and mildew-free branches, crush and sieve to obtain fine wood chips for later use;
[0023] 2) Choose wheat bran that has no mildew and no peculiar smell for use;
[0024] 3) Mix the fine wood chips, wheat bran, lime and gypsum in the proportion of the formula, sprinkle water and stir until there is no agglomeration and no particles to obtain the culture material a;
[0025] 4) Put the culture material a into the strain bottle, compact and punch holes, and sterilize for later use;
[0026] B. Inoculation and cultivation: Inoculate the fruiting body tissue pieces of Auricularia auriculae into the small holes of the culture material of the strain bottle, and then cultivate at a temperature of 15-22° C. to obtain the effective original species o...
Embodiment 1
[0048] (1) Weighing by weight: 94% wood chips, 5% wheat bran, 0.5% lime, and 0.5% gypsum.
[0049] (2) Mixing material: Stir the said dry material evenly, then sprinkle water, mix well, ensure no agglomeration and no particles, so that the water content reaches 60%.
[0050] (3) Sterilization by bottling: put the mixed culture material into a glass seed bottle, compact and punch holes, and sterilize.
[0051] (4) Inoculation culture: Under sterile conditions, use an inoculation needle to pick up a 1.5cm-sized Auricularia fruit body tissue block, inoculate it into the small hole of the culture material in the strain bottle, and place it in a constant temperature incubator at 18°C for cultivation after inoculation. .
Embodiment 2
[0053] (1) Weighing by weight: 95% wood chips, 4% wheat bran, 0.5% lime, and 0.5% gypsum.
[0054] (2) Mixing material: Stir the dry material evenly, sprinkle water, mix well, ensure no agglomeration and no particles, so that the water content reaches 62%.
[0055] (3) Sterilization by bottling: put the mixed culture material into a glass seed bottle, compact and punch holes, and sterilize.
[0056] (4) Inoculation culture: Under aseptic conditions, use an inoculation needle to pick up a piece of Auricularia fruiting body tissue with a size of 1.0 cm, inoculate it into the small hole of the culture material of the strain bottle, and place it in a constant temperature incubator at 19°C for cultivation after inoculation. .
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