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Probiotic preparation as well as preparation method and application thereof

A technology of probiotic preparations and compound enzyme preparations, applied in biochemical equipment and methods, methods based on microorganisms, applications, etc., can solve the problem of less research on dual fermentation technology, uneven product quality, and influence on product marketing, etc. problems, to achieve high fermentation bacteria, improve production efficiency, and inhibit the growth of miscellaneous bacteria

Inactive Publication Date: 2019-04-05
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, most of the products are in the form of liquid fermentation, but the cost is high, they are easily subject to feedback control, and it is difficult to increase the amount of viable bacteria
Moreover, after the liquid product is fermented, the strain is easily inactivated, resulting in a short shelf life of the product and affecting the marketing of the product
[0005] The application and development of Bacillus licheniformis in my country started relatively late. Therefore, although there are some Bacillus licheniformis products on the market, the number of effective viable bacteria is low, the production process is complicated and the cost is high, and the quality of the products is uneven, which affects the use. Effect
[0006] The single fermentation of the above-mentioned probiotics faces problems such as high fermentation cost, low number of viable bacteria, and many miscellaneous bacteria, while there are few researches on dual fermentation technology at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] The present invention provides a method for preparing a probiotic preparation, which includes the following steps in sequence:

[0034] (1) Preparation of liquid seed culture medium

[0035] Liquid Enterococcus faecium seed culture medium includes: peptone 10-20g / L, glucose 10-20g / L, the rest is water, pH 7.0-7.2, 1% ammonium citrate and urea mixture, ammonium citrate and urea The mass ratio is 1:1.

[0036] The liquid Bacillus licheniformis seed culture medium includes: peptone 10-20g / L, glucose 10-20g / L, the rest is water, and the pH is 7.0-7.2.

[0037] (2) Preparation of bacterial seed liquid

[0038] Enterococcus faecium and Bacillus licheniformis BL-09 were respectively inoculated into liquid Enterococcus faecium seed medium and liquid Bacillus licheniformis seed medium from the frozen glycerol tube. Place it on a shaker at a speed of 150-180r / min, a temperature of 30-37°C, and incubate for 20-24 hours.

[0039] (3) Preparation of solid fermentation medium

[0040] The soli...

Embodiment 1

[0050] The Enterococcus faecium and Bacillus licheniformis frozen in glycerin were inoculated into the liquid liquid Enterococcus faecium seed medium and the liquid Bacillus licheniformis seed medium. Place on a shaker, rotate speed 180r / min, temperature 37°C, and incubate for 24h.

[0051] Liquid Enterococcus faecium seed culture medium includes: peptone 15g / L, glucose 15g / L, the rest is water, pH 7.0, 1% ammonium citrate and urea mixture, the mass ratio of ammonium citrate to urea is 1:1 .

[0052] The liquid Bacillus licheniformis seed culture medium includes: peptone 15g / L, glucose 15g / L, the rest is water, and the pH is 7.0.

[0053] The solid-state fermentation medium includes the following components in weight fraction: 8 parts of soybean meal, 10 parts of wheat bran, 2 parts of corn flour, 1 part of mulberry leaves, and a water content of 50%.

[0054] Further, a solid-state fermentation medium was prepared according to the components provided in this example, and sterilized ...

Embodiment 2

[0059] Enterococcus faecium and Bacillus licheniformis BL-09 were inoculated into liquid liquid Enterococcus faecium seed medium and liquid Bacillus licheniformis seed medium, respectively. Place on a shaker, rotate speed 180r / min, temperature 37℃, and incubate for 20h.

[0060] Liquid Enterococcus faecium seed culture medium includes: peptone 10g / L, glucose 20g / L, the rest is water, pH is 7.2, a mixture of 1% ammonium citrate and urea, the mass ratio of ammonium citrate to urea is 1:1 .

[0061] The liquid Bacillus licheniformis seed culture medium includes: peptone 10g / L, glucose 20g / L, the rest is water, and the pH is 7.2.

[0062] The solid-state fermentation medium includes the following components in weight fraction: 8 parts of soybean meal, 10 parts of wheat bran, 2 parts of corn flour, 1 part of mulberry leaves, and a water content of 50%.

[0063] Further, a solid-state fermentation medium was prepared according to the components provided in this example, and sterilized at 1...

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Abstract

The invention provides a probiotic preparation as well as a preparation method and application thereof, which relates to the technical field of micro-ecological fermentation. The preparation method ofthe probiotic preparation comprises the following steps of: inoculating an enterococcus faecium seed solution and a bacillus licheniformis seed solution into a solid fermentation culture medium, adding a composite enzyme preparation for mixed fermentation, and preparing a fermentation product into the probiotic preparation. The invention provides a preparation method of the probiotic preparation.By adopting the preparation method of the probiotic preparation, the number of fermented viable bacteria is increased, the growth of miscellaneous bacteria is inhibited, the fermentation process is simplified, the production cost of microbial fermentation is reduced, and the production efficiency is increased. In addition, the invention also provides high protein feed, which can effectively enhance the body immunity of animals and promote the growth of the animals.

Description

Technical field [0001] The invention relates to the technical field of microecological fermentation, in particular to a probiotic preparation, a preparation method and its application. Background technique [0002] The addition of antibiotics to feed has made a significant contribution to the development of animal husbandry. At the same time, its side effects such as endogenous infections and double infections, the generation of drug resistance, the destruction of the normal intestinal flora, etc., are harmful to the breeding industry, animals and humans. Food safety poses a serious threat. Probiotics are mainly live microorganisms. After an animal takes a certain amount, a certain number of strains can adhere, colonize and grow in specific parts of the gastrointestinal tract. A microecological preparation is a preparation that mainly regulates the structure and balance of the intestinal microflora through probiotics, and plays a beneficial role in animal growth and health. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/01C12R1/10
CPCA23K10/18C12N1/20
Inventor 毛玲高智明陶敏周明李亚峥莫成周樱
Owner 湖北华扬科技发展有限公司
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