A method for three-dimensional microcarrier cell adsorption culture

A technology of cell adsorption and microcarriers, which is applied in general culture methods, tissue culture, biochemical equipment and methods, etc., can solve the problem of repair and regeneration function decline, and the two-dimensional microenvironment cannot simulate and reproduce the three-dimensional microenvironment well , stem cell survival rate and function reduction, to achieve the effect of promoting function, easy cultivation, and avoiding bacterial infection
CN109593704BActive Publication Date: 2022-02-11BEIJING CYTONICHE BIOTECH CO LTD
20 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING CYTONICHE BIOTECH CO LTD
Publication Date
2022-02-11

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a three-dimensional microcarrier cell adsorption culture method. It comprises the following steps: 1) inoculating cells: mixing the cell suspension with a dry three-dimensional microcarrier to obtain a microcarrier of the mixed cell suspension; 2) cell adsorption: mixing the mixed cell suspension obtained in step 1) The microcarriers are incubated by a rotational dynamic adsorption method, so that the cells adhere to the three-dimensional microcarriers; 3) Cell culture: after the cells are adhered in step 2), complete medium is added and cultured. The invention provides more growth area for cells, simplifies the operation of inoculating cells on microcarriers, and avoids the risk of bacterial contamination; it is suitable for the cultivation of large-scale bioreactors; the unique imbibition of three-dimensional microcarriers can be a large amount The cells enter the inside of the connected pores of the carrier, better form a bionic three-dimensional growth pattern, and promote the function of the in vitro cell culture process.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a three-dimensional microcarrier cell adsorption culture method, which belongs to the technical field of cell adsorption culture. Background technique

[0002] With the rapid development of interdisciplinary subjects such as biomedicine, materials science, mechanics, and engineering, more and more people are paying attention to the difference between the microenvironment of cells cultured in vitro and the microenvironment in vivo. The traditional two-dimensional cell culture (based on commercial culture dishes or multi-well plates) technology has been developed for more than 100 years, and it has been widely used in basic life science research, medical research and other fields. However, with the rapid development of microscopic imaging technology, researchers have gradually discovered the living state of cells in a two-dimensional culture environment, and the cell morphology is far from the culture conditions in the real envir...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More