System used for rescuing mumps virus and rescue method

A technology of mumps virus and helper plasmids, applied in the field of reverse genetics, which can solve problems such as complex systems and methods, difficult rescue, etc., and achieve the effect of shortening time and cost, and overcoming time-consuming and labor-intensive effects

CN109593784APending Publication Date: 2019-04-09ZHEJIANG UNIV
2 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2019-04-09

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a system used for rescuing a mumps virus and a rescue method, and belongs to the field of application of reverse genetics. The system comprises a recombinant transcription vector containing a whole genome cDNA (complementary deoxyribonucleic acid) of the mumps virus, a helper plasmid I containing an NP gene of the mumps virus, a helper plasmid II containing a P gene of themumps virus and a helper plasmid III containing an L gene of the mumps virus. The mumps virus can be effectively rescued from outside of a body by adopting a 3+1 plasmid rescue system (helper plasmidsand the transcription vector that are used for expressing an N protein, a P protein and an L protein of a MuV-S79 strain), the recombinant mumps virus can be abundantly amplified by a cell culture method, and the recombinant mumps virus can be directly used for manufacturing vaccines without any processing, so that the problems of the traditional vaccine that the production is time-saving and labor-saving, and the process is complex are solved. Additionally, a solution is provided for developing a novel vaccine by engineering the mumps virus by adopting a molecular clone technology in the future.
Need to check novelty before this filing date? Find Prior Art