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Method for cultivating phalaenopsis aphrodite Rchb. F

A method for cultivating phalaenopsis and a technique for phalaenopsis phalaenopsis, which is applied in the field of cultivating blooming cherry phalaenopsis, can solve the problems of no small pinkish red lips, excellent flower shape, good bifurcation and the like.

Inactive Publication Date: 2019-04-16
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the existing Phalaenopsis has many varieties, there are still no varieties with small powdered red lips, excellent flower shape and good branching property.

Method used

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  • Method for cultivating phalaenopsis aphrodite Rchb. F
  • Method for cultivating phalaenopsis aphrodite Rchb. F
  • Method for cultivating phalaenopsis aphrodite Rchb. F

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1) Hybrid seeds were obtained by cross-pollination with Phal. Jiaho Cherry as the female parent and Phal. Lobbii as the male parent;

[0037] 2) Cultivate the hybrid seeds (the temperature is 28°C, the relative humidity is 65%, and the light intensity is 10000lux) and the optimal plants are selected;

[0038] 3) Take the shoot tips of the optimal plants and soak them in alcohol for 25 seconds for disinfection, and then place them in the primary medium (the primary medium is MS medium + 0.2mg / L folic acid + 2.5mg / L 6-BA + 1.3mg / L The primary culture was carried out in mashed potatoes + 0.3mg / L ascorbic acid (cultivation time was 23 days, temperature was 25°C, light time was 11h / day, light intensity was 1800Lux) to obtain callus;

[0039] 4) Take the base of the buds on the callus and cut them into small pieces, then place them in the subculture medium (subsequent medium is MS medium + 0.2mg / L ABA + 1.9mg / L 6-BA + 0.4mg / L penicillin + 20g / L sucrose + 6g / L agar) for subc...

Embodiment 2

[0042] 1) Hybrid seeds were obtained by cross-pollination with Phal. Jiaho Cherry as the female parent and Phal. Lobbii as the male parent;

[0043] 2) Cultivate the hybrid seeds (the temperature is 25°C, the relative humidity is 70%, and the light intensity is 12000 lux) and the optimal plants are selected;

[0044] 3) Take the shoot tips of the optimal plants and soak them in alcohol for 20 seconds for disinfection, and then place them in the primary medium (the primary medium is MS medium + 0.1mg / L folic acid + 2mg / L 6-BA + 1.2mg / L potato The primary culture (cultivation time is 20 days, temperature is 23 ℃, light time is 10h / day, light intensity is 1500Lux) in primary culture in mud + 0.2mg / L ascorbic acid) to obtain callus;

[0045] 4) Take the base of the buds on the callus and cut them into small pieces, then place them on the subculture medium (subsequent medium is MS medium + 0.1mg / L ABA + 1.5mg / L 6-BA + 0.2mg / L penicillin+15g / L sucrose+4g / L agar) subculture (cultiv...

Embodiment 3

[0048] 1) Hybrid seeds were obtained by cross-pollination with Phal. Jiaho Cherry as the female parent and Phal. Lobbii as the male parent;

[0049] 2) Cultivate the hybrid seeds (the temperature is 30°C, the relative humidity is 60%, and the light intensity is 8000 lux) and the optimal plants are selected;

[0050] 3) Take the shoot tips of the optimal plants and soak them in alcohol for 30 seconds for disinfection, then place them in the primary medium (the primary medium is MS medium + 0.3mg / L folic acid + 3mg / L 6-BA + 1.5mg / L potatoes Callus was obtained by primary culture (culturing time was 25 days, temperature was 27°C, light time was 12h / day, light intensity was 2000Lux) in mud + 0.4mg / L ascorbic acid;

[0051] 4) Take the base of the buds on the callus and cut them into small pieces, then place them in the subculture medium (subsequent medium is MS medium + 0.1-0.3mg / L ABA + 2.2mg / L 6-BA + 0.6 mg / L penicillin+25g / L sucrose+8g / L agar) for subculture (cultivation time ...

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Abstract

The invention discloses a method for cultivating phalaenopsis aphrodite Rchb. F. The cultivating method comprises the steps: 1, using Phal. Jiaho Cherry as a female parent and Phal. Lobbii as a male parent to carry out hybrid pollination so as to obtain hybrid seeds; 2, the hybrid seeds are cultivated and optimal plants are selected out; 3, conducting tissue culture on the optimal plants to obtainthe phalaenopsis aphrodite Rchb. F. The phalaenopsis aphrodite Rchb. F has the characteristics of small pinked red lip petals, excellent flower shape and good bifurcation property, and meanwhile thecultivating method has the advantages of simple process and easy operation.

Description

technical field [0001] The invention relates to a method for cultivating phalaenopsis, in particular to a method for cultivating a spreading cherry phalaenopsis. Background technique [0002] Phalaenopsis aphrodite Rchb. F. is an epiphytic orchid, native to the subtropical rain forest. The thick white aerial roots of Phalaenopsis are exposed around the leaves, which not only absorb nutrients from the air, but also grow and photosynthesize. In the Spring Festival, Phalaenopsis plants pull out long flower stalks from the leaf axils, and bloom flowers like flying butterflies, which are favored by flower fans and are known as "Queen of Yang orchid". Distributed in Thailand, Philippines, Malaysia, Indonesia, and Taiwan. [0003] Although there are many varieties of Phalaenopsis, there are still no varieties with small pinkish red lips, excellent flower shape and good bifurcation. SUMMARY OF THE INVENTION [0004] The purpose of the present invention is to provide a kind ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 汪锡文裴宝平邱许凤赖本智田徐承玥
Owner 芜湖东源新农村开发股份有限公司
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