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Biological bacterial fertilizer, preparation method of biological bacterial fertilizer and application in prevention and treatment for southern blight and improvement of soil environments

A technology of biological bacterial fertilizer and bacterial cells, applied in the field of microorganisms, can solve problems such as reducing the effect of biocontrol bacterial fertilizer, and achieve the effects of overcoming the relatively large loss of microorganisms, promoting plant growth, and strong antagonistic effect.

Inactive Publication Date: 2019-04-16
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, after simple compounding of different bio-control bacteria, under appropriate temperature and humidity conditions, they can produce a strong antagonistic effect on each other, which will also reduce the effect of bio-control bacteria fertilizer

Method used

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  • Biological bacterial fertilizer, preparation method of biological bacterial fertilizer and application in prevention and treatment for southern blight and improvement of soil environments
  • Biological bacterial fertilizer, preparation method of biological bacterial fertilizer and application in prevention and treatment for southern blight and improvement of soil environments
  • Biological bacterial fertilizer, preparation method of biological bacterial fertilizer and application in prevention and treatment for southern blight and improvement of soil environments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Antagonism test between Bacillus amyloliquefaciens, Lysobacter yanansis SNNU513 and Sclerotinia neat

[0039] 1. Test process

[0040] (1) Transfer Sclerotinia sclerotiorum to PDA medium, respectively connect Bacillus amyloliquefaciens and Lysobacteryanansis SNNU513 to LB medium for activation culture, and carry out plate confrontation culture test after 3 days.

[0041] (2) The cultured Bacillus amyloliquefaciens, Lysobacter yanansis SNNU513 and Sclerotinia yanansis were respectively punched into a plate with a puncher with a diameter of 5 mm.

[0042] (3) Place the neat Sclerotinia plate in the center of the PDA plate (d=88mm), and place the Bacillus amyloliquefaciens plate / Lysobacter yanansis SNNU513 (Bacillus amyloliquefaciens plate / Lysobacteryanansis SNNU513 plate with Neat Sclerotinia discs are 2cm apart). Three replicates were set up for each treatment, and they were cultured upside down in a constant temperature incubator at 30°C.

[0043] (4) Obse...

Embodiment 2

[0046] Embodiment 2: Bacillus amyloliquefaciens and Lysobacter yanansis SNNU513 antagonistic test

[0047] 1. Test process

[0048] (1) Bacillus amyloliquefaciens and Lysobacter yanansis SNNU513 were respectively inoculated in LB liquid medium, and cultured at 37°C and 180r / min for 18-20h to prepare seed liquid.

[0049] (2) Divide the plate into two, draw a line in the middle, apply 100ml Lysobacter yanansis SNNU513 bacterial solution on one side, and 100ml Bacillus amyloliquefaciens solution on the other side, and observe whether antagonism occurs.

[0050] 2. Experimental results:

[0051] Such as figure 2 As shown, Bacillus amyloliquefaciens and Lysobacter yanansis SNNU513 do not antagonize and can be cultured together.

Embodiment 3

[0052] Example 3: Screening of Bacillus amyloliquefaciens and Lysobacter yanansis SNNU513 mixed culture inoculation sequence

[0053] 1. Test process

[0054] (1) Inoculate Bacillus amyloliquefaciens and Lysobacter yanansis SNNU513 respectively in LB medium to obtain seed solution.

[0055] (2) Take 1ml of Bacillus amyloliquefaciens bacteria liquid and add it into 100ml LB liquid culture medium. Take 1ml Lysobacteryanansis SNNU513 bacteria solution and add it to 100ml LB medium, and shake it well.

[0056] (3) Cultivate the bacterial liquid cultured in step (2) at 37°C and 180r / min, transfer 100ul bacterial liquid to a 96-well plate at regular intervals (2h / 12h), and measure the OD value at a wavelength of 620nm.

[0057] (4) Draw the strain growth curve.

[0058] 2. Experimental results

[0059] Such as image 3 and Figure 4 As shown, Lysobacter yanansis SNNU513 grew slowly ( image 3 ), Bacillus amyloliquefaciens grew faster ( Figure 4 ), so, first inoculate Lysoba...

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Abstract

The invention discloses a biological bacterial fertilizer, a preparation method of the biological bacterial fertilizer and application in prevention and treatment for southern blight and improvement of soil environments, and belongs to the technical field of microorganisms. The biological bacterial fertilizer is prepared from, by mass, 40 parts of embedded bacteria A and 60 parts of embedded mixedbacteria B, and through fermentation, embedding and drying, the biological bacterial fertilizer is prepared. The fertilizer not only can inhibit southern blight, but also can improve the soil microorganism environment and adsorb heavy metal in soil. The embedding method is adopted for solving the problem that in the actual production process, the loss of microorganisms is larger, the fertilizer is not easy to store, and the content of effective bacteria is too low, and the using efficiency of the bacterial fertilizer is improved. Compared with traditional prevention and treatment adopting chemical pesticide, the bacterial fertilizer has the advantages that there are no pesticide residues, and the biological bacterial fertilizer is environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a biological bacterial fertilizer and a preparation method thereof, as well as the application of preventing and treating bacterial canker sores and improving soil environment. Background technique [0002] Silkworm is a kind of soil-borne disease with high incidence and great harm in agriculture. The asexual form of the pathogenic bacteria that causes canker sores is Sclerotium rolfsii. The pathogen was first discovered in the United States in 1891 when tomato was infected with canker sores, and since then there have been reports of different crops infected with canker sores all over the world. Existing studies have shown that the plant hosts of S. albicans are diverse, involving nearly 600 species of plants, widely distributed in crops, vegetables, flowers and Chinese medicinal materials. The reported hosts include corn, soybean, peanut, cotton, cotton, potato, aconite, eggpla...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05G3/04C12N11/10C12N11/04C09K17/32A01N63/02A01P3/00C09K101/00C12R1/01C12R1/38C05G3/80
CPCA01N63/10C05F11/08C05G3/80C09K17/32C09K2101/00C12N11/04C12N11/10
Inventor 肖光辉陈佳阳王丽娜付盟王冬阁冯正平闫亚平崔浪军
Owner SHAANXI NORMAL UNIV
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