34 results about "Microbial loop" patented technology

The invention relates to the technical field of microbial fermentation control, in particular to an automatic microbial reaction fermentation control system and a control method. The method comprises the following steps: collecting real-time microbial environment parameters and identifying a data multi-axis structure, extracting master control environment interval data, eliminating redundant signals to generate standard microbial environment data, defining a fermentation degree contrast template through fermentation historical records, and constructing a microbial fermentation timeline framework. The method comprises the following steps: acquiring standard environmental data, identifying a fermentation state of the standard environmental data, constructing a simulated microbial environmental field by using the standard microbial environmental data, performing global microbial reaction simulation, determining fermentation reaction structure partitions based on the data, performing difference comparison to generate fermentation difference data, and performing compensation simulation by using the difference data. And automatic fermentation compensation control of microorganisms is realized. According to the invention, the intelligent automatic control and optimization of the microbial fermentation process are realized, the fermentation efficiency and stability are obviously improved, and the manual intervention is reduced.

The invention provides an intelligent sensor cooperative control method and system for a microbiological detection environment, and relates to the technical field of sensor control, and the method comprises the steps: carrying out the hierarchical cloud edge control architecture layout of a production workshop according to equipment distribution information and production activity information, and obtaining a cascaded hierarchical control network; the K-level cooperative control secondary center executes partition autonomous parameter mapping and outputs a K-layer distributed environment compensation strategy; the microbial environment cooperative control center carries out transition area buffer control and outputs a global cooperative control strategy; and the microbial environment cooperative control center issues a global cooperative control strategy to the environment stability maintaining equipment array based on the spatial topological relevance of the environment stability maintaining equipment, and low-fluctuation environment steady state maintenance is carried out. The technical problem that the stability of a microbiological detection environment is affected due to the fact that most microbiological detection environment adjusting systems in the prior art are based on fixed parameter setting and lack of a quick response mechanism for sudden environment changes is solved.

The invention belongs to the technical field of immobilized microorganisms and microbial environment restoration, and particularly relates to a chitosan modified charcoal immobilized complex microbial inoculant as well as a preparation method and application thereof. The chitosan modified biochar immobilized complex microbial inoculant provided by the invention comprises complex bacteria, biochar and chitosan, the complex bacteria are fixedly attached to the surface of the biochar and the interiors of pores, and the chitosan forms a layer of uniform membrane structure on the surface of the biochar. The chitosan modified biochar immobilized complex microbial inoculant not only improves the viable count and stability of immobilized microorganisms, but also can significantly improve the degradation rate of pollutants, has good acid-base tolerance and salt tolerance, can be suitable for pollutant degradation and environment restoration or improvement in various acid-base environments and salt environments, and has wide application prospects. The method has good environmental adaptability and good application prospects.

The invention relates to the field of operating room ventilation, in particular to a ventilation and purification control system based on an intelligent operating room, and aims to solve the problem that a ventilation system running at constant power cannot cope with a complex and changeable dust and bacterium environment, so that the ventilation and purification efficiency is inappropriate. Comprising an air volume management and control unit, a purification rate management and control unit, a ventilation task management unit, a ventilation environment acquisition unit, a purification environment supervision unit and a purification equipment supervision unit. According to the invention, intelligent analysis is carried out according to the dust environment in the operating room and the idle time of the operating room, so that the purification work of the operating room does not influence the normal use of the operating room, the microbial environment in the operating room is monitored, and the operation of the sterilization equipment is intelligently controlled according to the microbial monitoring result; quantitative analysis is carried out according to the dust environment and the microbial environment, air volume control is achieved, the air volume can meet the rapid ventilation requirement of the dust environment, and the ventilation speed can be controlled to guarantee the sterilization effect.

The invention relates to the technical field of soil remediation, in particular to an efficient in-situ microbial remediation method for organic contaminated soil, which comprises the following steps: layering and dividing the contaminated soil into layers and regions, and injecting different medicaments into circulating well groups positioned in different regions to achieve the purpose of adjusting an underground microbial environment, and automatically forming and constructing an aerobic-anaerobic transition zone. Meanwhile, self-circulation of underground water is accelerated through the circulating well group, different indigenous microorganism florae are activated, and a metabolic chain is formed; and aerobic / anaerobic microorganisms are periodically injected into the injection well, an electron donor and a carbon source are synchronously provided, the steady state of an ecosphere is maintained, and the aims of optimizing the construction period and the treatment effect of the in-situ bioremediation technology of the polluted soil and the underground water are achieved.

The invention relates to the technical field of ecological equipment, and particularly discloses an ecological toilet suitable for locomotive drivers and a control method of the ecological toilet. The ecological toilet comprises a fermentation tank, microbial thalli in the fermentation tank, a heating device, a stirring device, a temperature sensor, a humidity sensor, a high-frequency vibration generator and a control assembly; the high-frequency vibration generator is mounted on the humidity sensor and used for cleaning the humidity sensor, determining the optimal biological activity parameters of the microbial thalli through the temperature sensor and the humidity sensor, and controlling the stirring device to execute corresponding stirring actions based on the optimal biological activity parameters. Accurate humidity data are collected through the humidity sensor, so that the microbial environment in the ecological toilet is accurately controlled, and the biodegradation effect is improved.

The present invention discloses an intelligent Juncao transplanting method and system based on Internet of Things technology, which relates to the field of intelligent agricultural technology. The invention collects key environmental data such as soil microbial environment, wind speed and pressure, mechanical stress, soil moisture and light conditions in real time, and uses convolutional neural network technology to establish a transplanting environment prediction model. This can screen the Juncao transplanting adaptability of each sub-region, promote the improvement of transplanting success rate, and optimize resource allocation. During the growth process of Juncao transplanting, data on the compatibility of root exudates and pest attraction effects are continuously collected to establish a dynamic growth data set, and the growth risk coefficient is monitored in real time. This process can timely detect adverse trends during growth and ensure that countermeasures are taken when problems first appear. According to the real-time risk assessment results, the environmental optimization strategy is adjusted to ensure the healthy growth of Juncao throughout the growth cycle and reduce unnecessary resource waste.

The present invention relates to the technical field of sewage treatment, and in particular to a device and method for low-carbon and high-efficiency synchronous denitrification and phosphorus recovery of sewage. The method of the present invention realizes the coupling of short-range denitrification, anaerobic ammonia oxidation and biomineralization, and realizes the removal of nitrogen in sewage through short-range denitrification and anaerobic ammonia oxidation, and realizes the removal of phosphorus in sewage through biomineralization; while removing phosphorus, hydroxyapatite is obtained, and hydroxyapatite can be used as the core of granular sludge to ensure the stability of granular sludge, and an aerobic-anoxic-anaerobic microbial environment is formed on the granular sludge, which is conducive to the reduction of sludge; at the same time, the reaction equipment discharges sludge regularly, and the flocculent sludge and the core in the granular sludge can be separated by a dispersion device and a separation device, and the separated core can be utilized as a resource, and the separated flocculent sludge is concentrated and returned to the fermentation area, and the carbon source can be supplemented after fermentation to achieve sludge reduction.

The invention relates to the technical field of citrus cultivation, and discloses a compound substrate formula for promoting healthy growth of citrus root systems and a preparation method of the compound substrate formula. A formula of the compound substrate for promoting healthy growth of citrus root systems comprises the following raw materials in parts by weight: 20-30 parts of traditional Chinese medicine residues, 15-25 parts of edible fungus waste, 10-20 parts of crop straws, 8-15 parts of livestock and poultry manure, 5-10 parts of distillers' grains, 5-10 parts of vinegar residues, 3-8 parts of shell powder, 2-6 parts of mineral humic acid powder, 3-7 parts of a ternary compound fertilizer, 0.5-2 parts of an EM strain and 1-4 parts of a seaweed extract. According to the formula of the compound substrate for promoting healthy growth of the citrus root system, various nutrient elements are provided through the traditional Chinese medicine residues and the like, and the rhizosphere microbial environment is adjusted in combination with the EM strain, so that the problem that currently common citrus culture substrates mostly adopt single chemical fertilizers or simple organic materials; therefore, the problems of unbalanced nutrient absorption and limited growth of the root system are solved.

The utility model discloses a biogeochemical cycle experiment device for nitrogen in a water-containing medium, which comprises a cylinder body, a plurality of samplers, a first filling layer, a water injection component and the like, before use, after the first filling layer is filled in the cylinder body, the first filling layer is positioned in a light-proof section, so that the underground dark environment is simulated; during use, the water injection assembly is used for injecting irrigation water into the water inlet, the water gradually flows through the first filling layer and then is discharged from the water outlet in the lower portion of the barrel, and therefore the underground environment containing the microorganisms is well simulated. A user obtains water near the sampling holes at different heights as samples through the samplers at the sampling holes, so that soil solutions in soil at different depths in a real bottom layer are simulated and collected, and the sampling holes can be used for sampling soil on the premise of simulating the microbial environment of the real soil layer. The concentration of the nitrogen-containing compound to be detected in the water-containing media with different depths and the nitrogen conversion function gene parameters are researched.

The application provides a sealed microbial culture device and a microbial culture method. The device comprises a sealed culture box, an environment control assembly, an air inlet assembly, a liquid inlet assembly and a sampling assembly. The sealed culture box is transparent and used for observing and culturing microorganisms. The environment control assembly is used for collecting and adjusting the environmental parameters in the sealed culture box. The air inlet assembly is used for providing gas into the sealed culture box. The liquid inlet assembly is used for providing liquid into the sealed culture box. The sampling assembly is arranged on the outer wall of the sealed culture box and used for extracting or discharging the gas or liquid in the sealed culture box. The application can realize the culture of microorganisms in a specific sealed environment, can adjust the environmental factors, gas and liquid factors in the sealed culture box, and avoids the influence of the gas extraction and liquid extraction process on the culture environment. The application has the characteristics of convenient operation, easy cleaning, good sealing, high use efficiency and continuous experiment.

The utility model relates to the technical field of water quality purification, and discloses a constructed wetland water quality purification structure which comprises a side slope, a reinforcing layer, a filler layer, a planting soil layer, a planting pore plate and plants, the reinforcing layer is laid on the side slope, the filler layer is laid on the reinforcing layer, the side slope is reinforced through the reinforcing layer, and the planting pore plate is arranged on the planting soil layer. Then, a filler layer is arranged on the reinforcing layer and used for creating a microbial environment, a side slope is elevated, a planting soil layer and a planting hole plate are arranged and used for planting plants, a water storage bin is arranged and used for collecting overflowing water, and water in the water storage bin permeates into the filler layer again through a water seepage opening in the water storage bin and then is purified; when the water flow is large, overflowing water enters the water storage bin from the water inlet, when the water flow is small, under the action of the water pressure difference, the water in the water storage bin permeates into the filler layer from the water seepage opening, after the filler layer is filled with water, the water overflows, and it is ensured that plants have enough water to survive.

The invention discloses a method for preparing a soil conditioner from fly ash and application, and belongs to the field of soil improvement and solid waste recycling, the method comprises the steps of fly ash grading screening, microwave-mechanical synergistic activation, acid-base modification, formula compounding, secondary biological activation and forming post-treatment, and the soil conditioner is prepared through multi-element activation and compounding. The activity and pore structure of the fly ash are improved. According to the method, the raw material utilization rate is high, the energy consumption is reduced by over 30%, the cost is low, and the prepared conditioner can remarkably reduce the pH and the total salt content of the saline-alkali soil, increase the porosity, organic matter and nutrients of the soil and improve the microbial environment; and the special formula can be used for promoting the growth of crops and increasing the yield by 15-20%, so that the special fertilizer is easy to industrially popularize and has both environmental and economic benefits.

This invention relates to the field of ecological equipment technology, specifically disclosing an ecological toilet suitable for locomotive drivers and a control method for the ecological toilet. The toilet includes a fermentation tank and microbial cells within it, a heating device, a stirring device, a temperature sensor, a humidity sensor, a high-frequency vibration generator, and control components. The high-frequency vibration generator is mounted on the humidity sensor for cleaning it. The optimal bioactivity parameters of the microbial cells are determined using the temperature and humidity sensors, and the stirring device is controlled to perform corresponding stirring actions based on these optimal parameters. Precise humidity data is collected by the humidity sensor, thereby accurately controlling the microbial environment within the ecological toilet and improving the biodegradation effect.

The invention relates to a method for preparing peanut shell biochar in a carbon dioxide atmosphere to strengthen chicken manure anaerobic digestion, and belongs to the technical field of agricultural waste treatment. In order to solve the problem that the ammonia inhibition relieving effect of common charcoal in the anaerobic digestion process of chicken manure is limited, the invention provides a method for preparing peanut shell charcoal in a carbon dioxide atmosphere to strengthen anaerobic digestion of chicken manure. Dried and crushed peanut shells are placed in a carbon dioxide atmosphere, heated to a pyrolysis temperature at a certain heating rate, and cooled to room temperature after heat preservation for a certain time to obtain the peanut shell biochar, the porosity and the specific surface area of the biochar are remarkably improved, the porous structure of the peanut shell biochar is effectively optimized, the biochar is used for anaerobic digestion of chicken manure, and the yield of chicken manure is increased. Ammonia inhibition can be relieved through multiple mechanisms of promoting interspecific electron transfer, improving the microbial environment, enhancing the adsorption effect and the like, the recycling value of agricultural waste is increased, and a sustainable solution is provided for anaerobic digestion of high-nitrogen organic waste.

The invention discloses a multi-dimensional quantitative detection method for microbial corrosion resistance of Cr-containing steel. The method comprises the following steps: a microbial challenge test: inoculating sulfate reducing bacteria into a culture medium, and exposing a sample in a microbial environment; performing quantitative analysis on corrosion products: performing ultrasonic rust removal on the sample by using an acid pickling solution, then calculating a weightlessness corrosion rate, and quantifying the adhesion amount of microorganisms on the surface of the sample; multi-mode electrochemical testing: monitoring the open-circuit potential and linear polarization resistance of the sample, and performing electrochemical impedance spectroscopy and potentiodynamic polarization curve testing to obtain corrosion current density and polarization resistance; surface analysis: analyzing the corrosion morphology and element distribution of the sample through a scanning electron microscope and an energy disperse spectroscopy, and determining the chemical state of the chromium element by adopting X-ray photoelectron spectroscopy to obtain the stability and the sulfur content of a passive film; and evaluating the corrosion degree: establishing a coupling model of the corrosion current density, the polarization resistance, the passivation film stability, the microorganism adhesion amount and the sulfur content, and calculating the corrosion degree.

The invention is suitable for the cross technical field of agricultural environment microbial ecology and heavy metal pollution remediation, and provides a method for analyzing a rhizosphere microdomain microorganism driving mechanism for regulating cadmium accumulation of oilseed rape through a selenium fertilizer. And the physical separation of rhizosphere and non-rhizosphere soil is realized by synchronously adopting a special rhizosphere compartment micro-area device. By measuring the Cd / Se content of plants and soil, the rhizosphere microbial community structure, the functional gene (dsrA) abundance and microorganism-environmental factor correlation analysis, the system reveals that the selenium fertilizer (500-1000g Se / hm < 2 >) drives rhizosphere to enrich key functional microorganisms such as desulfothiones and geobacteria, and the sulfate reducing capacity of the selenium fertilizer (500-1000g Se / hm < 2 >) is remarkably improved (the abundance of the dsrA gene is increased by 250%); therefore, a microbial mechanism of cadmium bio-availability is reduced. The invention provides methodological and theoretical support for farmland heavy metal pollution remediation by utilizing microbial regulation and control.

The invention belongs to the field of aquaculture, and particularly relates to a two-crop freshwater shrimp healthy ecological breeding method. The healthy ecological freshwater shrimp breeding method comprises the steps of pond preparation, shrimp seed stocking, breeding management, daily management, fishing and harvesting and the like. In the whole breeding process, breeding input products mainly including microbial feed, microbial environment improver and plant extract feed additive are adopted for ecological breeding, the purposes of reducing the dosage for breeding and replacing resistance and avoiding resistance are achieved, and the quality safety of the freshwater shrimps is guaranteed. Meanwhile, the culture water body environment is subjected to in-situ purification, culture tail water pollution is reduced, up-to-standard discharge can be achieved, and remarkable economic, social and ecological benefits are achieved.

The invention relates to the technical field of cellars, and particularly discloses a cellar masonry and maintenance method which specifically comprises the following steps: preparing strip stones and a joint mixture; the joint mixture comprises glutinous rice flour, slaked lime, wheat protein and water; the strip stones are made of red sand stones; constructing a yellow ditch and a base layer; carrying out masonry construction on the base layer by taking strip stone as a masonry material and matching with a joint mixture to finish cellar construction; and maintaining. Compared with the prior art, the method has the advantages that the prepared joint mixture is matched with strip stones to build the pit, so that the problem that the fermentation quality of fermented grains and the flavor of wine are influenced by alkaline substances is effectively avoided; after the pit is built, the pit is maintained, so that a stable microbial environment can be formed in the pit.

The utility model discloses a kind of biogas slurry fertilizer aerobic fermentors, involve biogas slurry processing technical field.Its structure includes flatbed cart and the fermentation tank, air pump and sewage pump installed on flatbed cart;S-shaped arrangement's aeration gas guide pipe is arranged in the bottom of fermentation tank, and aeration gas guide pipe one end is connected with air pump air outlet after being through fermentation tank;Aeration gas guide pipe is equipped with several aeration heads;One row of hanging rods is fixed between one relative inner wall of fermentation tank upper end portion;One row of fiber bundle fillings is installed on hanging rod.The utility model is combined by flatbed cart, fermentation tank, air pump and sewage pump to strengthen microbial decomposition, improve biogas slurry quality and safety, guarantee biogas tank stable operation, promote resource utilization and handle substandard biogas slurry in time, maintain biogas tank inner microbial environment balance, guarantee biogas continuous and stable generation;Equipment is flexible and efficient, and strong adaptability, and operation is simple and convenient, maintenance cost is low, effectively solve rural biogas slurry processing problem.

The invention belongs to the technical field of macrobrachium rosenbergii breeding, and particularly relates to a method for breeding and hatching seedlings of macrobrachium rosenbergii in a tropical high-temperature region, which comprises the following steps: (1) selecting parent shrimps; (2) breeding parent shrimps; (3) breeding; according to the method, the adaptability of offspring to high temperature and severe water quality is improved from the gene level by utilizing the breeding advantage of a pseudo-female population and the ammonia-nitrogen-nitrite-resistant characteristic of male shrimps, the female-male ratio is controlled to be 1.8: 1-1.6: 1, the stocking density is smaller than or equal to 13 shrimps / m < 2 >, competition pressure and water quality deterioration caused by too high density are avoided, the mating probability is increased through reasonable matching, and the survival rate is increased. Liquorice and biological flocs are laid in an open-air cement pond, the substrate and microorganism environment is improved, pond temperature control is combined with precise temperature difference control, the physiological needs of parent shrimps are matched, the river micro-flow environment is simulated, parent shrimp gonad hormone secretion is promoted, and the breeding activity is stimulated.

The invention provides a preparation method of a metal ion bridged fulvic acid-polypeptide ternary composite material, and relates to the field of fertilizers, and the preparation method comprises the following steps: S1, preparing a fulvic acid solution; s2, preparing a polypeptide solution: dissolving polypeptide in a buffer solution, and stirring to prepare the polypeptide solution; s3, preparing a metal ion solution: adding metal salt into a second solvent, and stirring to prepare a metal salt solution; s4, under the stirring condition, the metal salt solution is added into the fulvic acid solution for a reaction, and a composite solution is formed; s5, adding a polypeptide solution into the composite solution under a stirring condition to obtain a mixed solution; s6, carrying out centrifugal separation on the mixed solution, collecting the precipitate, and washing the precipitate; and step S7, performing vacuum drying on the washed precipitate to obtain the metal ion bridged fulvic acid-polypeptide ternary composite material. According to the invention, a three-dimensional network structure is constructed through bridging of metal ions, so that the composite material is more stable in soil acid-base and microbial environments.

The present invention relates to the field of operating room ventilation, and is used to solve the problem that a ventilation system operating at a constant power cannot cope with complex and changeable dust and bacteria environments, resulting in inappropriate ventilation and purification efficiency. Specifically, it is a ventilation and purification control system based on an intelligent operating room, including an air volume control unit, a purification rate control unit, a ventilation task management unit, a ventilation environment acquisition unit, a purification environment supervision unit, and a purification equipment supervision unit; in the present invention, intelligent analysis is carried out according to the dust environment inside the operating room and the idle time of the operating room, so that the purification work of the operating room will not affect the normal use of the operating room, the microbial environment in the operating room is monitored, the operation of the sterilization equipment is intelligently controlled according to the microbial monitoring results, and quantitative analysis is carried out according to the dust environment and the microbial environment to achieve air volume control, so that the air volume can not only meet the rapid air change requirements of the dust environment, but also control the ventilation speed to ensure the sterilization effect.

The present invention relates to the technical field of cutting fluid recycling, and specifically relates to a cutting fluid recycling system, which includes a filtration tank. An inlet water pipe, an outlet water pipe, a sludge discharge port and a controller are provided on the filtration tank. The system also includes a detection device. A detection device for detecting the internal microbial environment of the filtration tank is installed on the filtration tank. An antibacterial device for cooperating with the detection device to inhibit microorganisms is installed on the filtration tank. The detection device includes a detection tube installed on the filtration tank, and a PH monitor is provided at one end of the detection tube located outside the filtration tank. The present invention measures the bacterial environment through a PH-type automatically calibrated detection device. When the PH value of the cutting fluid exceeds the set value, a gas-guided feeding antibacterial device that matches the detection device is used to destroy the environment suitable for the survival of microorganisms in the cutting fluid recycling system, including oxygen content, PH value, etc., and more comprehensively curb the survival and reproduction of microorganisms.

The invention provides an automatic optimization method for regulating and controlling key influence factors of gastrodia elata germination bacterium fermentation liquor, which comprises the following steps: designing an online experimental platform to obtain an original experimental data set; a plurality of micro sensors are integrated on the reaction unit, and data acquisition, image analysis, feature extraction, physical and chemical parameter inference and microorganism multi-component detection and activity online analysis are performed to obtain multi-dimensional biological index data; the method comprises the following steps: collecting a fermentation liquor sample, and treating and analyzing the collected sample to obtain a microorganism-environment-metabolism collaborative network; a multi-target comprehensive evaluation function and a multi-target prediction model are constructed, influences of different factors are predicted, and an optimal parameter solution set and a factor and result interpretable report are obtained; and carrying out data storage and management, and carrying out model iterative optimization. Accurate control and efficient optimization of key influence factors of the fermentation liquor are achieved, experiments and fields are integrated, the production efficiency is improved, and large-scale expansion of gastrodia elata regulation and control is promoted.

The invention provides a submerged plant-ecological interface system for in-situ treatment of water bodies, which provides a functional microbial community and fertilizer for the growth of submerged plants, so that the submerged plants are rooted in the ecological interface to form a plant rhizosphere microbial environment with the ecological interface, and a submerged plant-ecological interface system is constructed, which can effectively improve the problems of high planting environment requirement, large construction difficulty, low planting survival rate, slow water purification effect, high maintenance cost and the like faced by the existing submerged plant purification water body; the plants and microorganisms are organically combined together to form an efficient biological repair composite, which has a significant pollution removal, water purification and water body ecological restoration effect, and has a good application prospect in the field of water pollution treatment.

The utility model discloses a vaporized hydrogen peroxide disinfection parameter detection system, which relates to the technical field of microbial environment disinfection and sterilization and comprises a host and a plurality of detectors, an air inlet is formed in one end of the detector, an air outlet is formed in the other end of the detector, an air passing channel is formed in the detector, the air inlet is formed in one end of the air passing channel, the air outlet is formed in the other end of the air passing channel, and a control panel, a temperature and humidity detection probe and a hydrogen peroxide detection probe are fixedly arranged in the detector. The temperature and humidity detection probe and the hydrogen peroxide detection probe extend into the air passing channel and are in communication connection with the control panel, and the control panel is in communication connection with the host; according to the utility model, all-directional and dead-corner-free data acquisition and detection can be realized.

The utility model discloses a boiler blow-off heat energy recovery and aeration regulation combined system which comprises a boiler, a flash tank, a first heat exchange unit, a regulating tank and an aerobic tank, a blow-off pipe of the boiler is communicated with the flash tank, a first heat exchange unit is arranged in the flash tank, the inlet end of the first heat exchange unit is communicated with air through a first air blower, and the outlet end of the first heat exchange unit is connected with two distribution branches through a second air blower; the first branch is communicated with the aerobic tank through a main valve, and the second branch is communicated with the regulating tank through a regulating valve. According to the boiler blow-off heat energy recovery and aeration regulation combined system, blow-off waste heat can be effectively utilized for controlling the temperature of the aerobic tank, and a high-quality microbial environment temperature is created.

The application discloses a mulberry growth environment evaluation method based on rhizosphere microorganism data identification, and the method comprises the following steps: arranging monitoring points according to root system distance in a mulberry planting area, collecting rhizosphere microorganism identification data and environmental parameters of each monitoring point in a historical period; adopting multi-period parameter extraction to construct a data item set, and utilizing an FP-Growth algorithm to mine microorganism-environment correlation items; extracting time series data based on the correlation items, generating a prediction sequence of microorganisms and environmental parameters through an ARIMA model, combining with a mulberry growth condition to evaluate an ecological change trend, and implementing a forward and reverse microorganism regulation scheme according to mulberry growth feedback. The application fuses microorganism data and environmental parameters for correlation analysis, establishes a dynamic rhizosphere microorganism evaluation process, can accurately predict the evolution trend of the planting environment and actively regulate the rhizosphere microorganism, improves the predictability and environmental adaptability of mulberry growth regulation, and provides accurate scientific decision support for agriculture.

This invention provides a reporter phage, its construction method, and its applications. The reporter phage comprises a nucleic acid sequence encoding a reporter protein and at least one receptor-binding protein, wherein the receptor-binding protein is partially or entirely engineered to enable the reporter phage to specifically infect specific capsular types of Klebsiella pneumoniae. The reporter phage provided by this invention enables sensitive and specific detection of Klebsiella pneumoniae in complex genetic and multi-microbial environments. In particular, the scalable and iteratively evolving reporter phage platform provided by this invention offers a universal diagnostic tool for addressing capsular diversity, guiding phage-based therapies, and providing information for epidemiological surveillance.