Construction method and detection method of uplc fingerprint of Angong Niuhuang Pills

A technology of Angong Niuhuang Pills and fingerprints, which is applied in the field of quality control of traditional Chinese medicine, can solve the problems of small number of characteristic peaks, long time, difficult quality evaluation of Angong Niuhuang Pills, etc., achieve high accuracy, short time, and improve quality detection efficiency effect

Active Publication Date: 2022-01-14
GUANGZHOU BAIYUNSHAN ZHONGYI PHARMA COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars have used high performance liquid chromatography and gas chromatography to establish the fingerprints of Angong Niuhuang Pills, but it takes a long time and the number of characteristic peaks is small, so it is difficult to evaluate the quality of Angong Niuhuang Pills efficiently and comprehensively.

Method used

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  • Construction method and detection method of uplc fingerprint of Angong Niuhuang Pills
  • Construction method and detection method of uplc fingerprint of Angong Niuhuang Pills
  • Construction method and detection method of uplc fingerprint of Angong Niuhuang Pills

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Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment is a method for constructing UPLC fingerprints of Angong Niuhuang Pills.

[0039] 1. Materials

[0040] 1.1 Instrument

[0041] ACQUITY UPLC H-Class ultra-high performance liquid chromatography (Waters Shanghai Technology Co., Ltd.); Agilent G6500UPLC tandem quadrupole time-of-flight mass spectrometer (Agilent Technologies Co., Ltd.); Waters Xevo G2-Q-TOF (Waters Shanghai Technology Co., Ltd.) Co., Ltd.); BX8200HP Ultrasonic Apparatus (Shanghai Xinmiao Medical Instrument Manufacturing Co., Ltd.); Sartorius BP211D 1 / 100,000 Electronic Analytical Balance (Sartorius, Germany); Ultrapure Water System (Millpore, USA).

[0042] 2. Methods and results

[0043] 2.1 Fingerprint establishment

[0044] 2.1.1 Chromatographic conditions

[0045] Chromatographic column: Poroshell 120EC-C18 (2.1*100mm, 2.7μm); mobile phase: methanol (B)-volume concentration 0.1% formic acid water (A); elution gradient (volume fraction): 0-6min 10%-25% B, 6-22min25%-46%B, 22-24min ...

Embodiment 2

[0092] This embodiment is a detection method of Angong Niuhuang Pills, the steps are as follows:

[0093] 1. Chromatographic conditions

[0094] See item "2.1.1 Chromatographic conditions" in Example 1.

[0095] 2. Preparation of sample solution to be tested: Take Angong Niuhuang Pills to be tested (batch number: X02018), cut into pieces, take about 0.5g, accurately weigh it, put it in a 50mL conical flask with a stopper, and accurately add methanol with a volume concentration of 75% 25mL, packed tightly, weighed, mixed evenly, treated with ultrasonic (400W, 40kHZ) for 60min, allowed to cool, weighed again, supplemented the lost weight with methanol with a volume concentration of 75%, shaken well, let stand, the supernatant Filter through a microporous membrane (0.22 μm), and take the subsequent filtrate, that is, the obtained product.

[0096] 3. Determination: Precisely draw 2 μL of the sample solution to be tested, inject it into an ultra-high performance liquid chromatog...

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PUM

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Abstract

The invention relates to a UPLC fingerprint spectrum construction method and detection method of Angongniuhuang Pills. The method for constructing UPLC fingerprints includes the following steps: preparation of reference substance solution: take geniposide reference substance, add solvent to dissolve, and obtain the reference substance solution; preparation of test solution: take Angong Niuhuang Pill sample, add volume concentration Extract for 70~80% methanol aqueous solution, filter, get continued filtrate, obtain described need testing solution; Fingerprint spectrum construction: inject described reference substance solution and need testing solution in the ultra-high performance liquid chromatograph respectively, Determination, the fingerprint spectrum is obtained. The construction method calibrated 25 common peaks and identified 17 chromatographic peaks within 30 minutes. The method is simple, fast, accurate and reproducible. scientific basis.

Description

technical field [0001] The invention relates to quality control of traditional Chinese medicines, in particular to a method for constructing UPLC fingerprints and a detection method for Angongniuhuang Pills. Background technique [0002] Angong Niuhuang Pill comes from "Tiaodian of Febrile Diseases" written by Wu Tang, a physician in the Qing Dynasty. The effect of detoxification, eliminating phlegm and resuscitation is widely used in the treatment of stroke, coma, ischemic hypoxic encephalopathy, hypertensive cerebral hemorrhage, etc., and the curative effect is accurate and reliable. Modern pharmacological studies have shown that Angong Niuhuang Pills have good anticonvulsant, brain injury protection, anti-inflammatory and detumescent effects, and can reduce experimental hypertension in animals. At the same time, it can also enhance the body's immune response, and can improve whole blood viscosity, plasma Viscosity, which plays a role in protecting the brain injury of isc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 叶文才柴玉爽范春林何源峰曾瑚瑚宁娜苏碧茹张春波尹震
Owner GUANGZHOU BAIYUNSHAN ZHONGYI PHARMA COMPANY
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