A method for detecting the concentration of ticagrelor and its active metabolites in human plasma

A technology for ticagrelor and metabolites, applied in the field of drug analysis, can solve problems such as unfavorable promotion, expensive equipment, and small laboratory coverage, and achieve the effects of good specificity, high accuracy, and rapid detection process
CN109633063BActive Publication Date: 2021-05-04HARBIN MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HARBIN MEDICAL UNIVERSITY
Publication Date
2021-05-04

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Abstract

The invention discloses a method for detecting the concentration of ticagrelor and its active metabolites in human plasma, which comprises the following steps: (1) preparation of a standard working solution; (2) sample processing; (3) preparation of a standard curve; 4) Quantitative analysis: take the test sample and process it according to the method of step (2), and calculate according to the standard curve equation obtained in step (3), to obtain the concentration of ticagrelor and its active metabolite in the sample to be tested. The present invention uses high-performance liquid chromatography (HPLC) to measure the concentration of ticagrelor and its active metabolites in human plasma, and accurately quantifies the concentration of ticagrelor and its active metabolites in human plasma. Reasonable selection of methods, mobile phases, mass spectrometry conditions, etc., optimization of experimental conditions such as flow rate, mobile phase ratio, and chromatographic conditions, etc. After a series of methodological verifications, the entire quantitative detection method is practical, easy to operate, high in repeatability, and good in accuracy , strong operability, and can be directly applied to the detection and analysis of plasma samples of clinical patients and related pharmacokinetic analysis.
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Description

technical field

[0001] The invention relates to a method for detecting the concentration of ticagrelor and its active metabolites in human plasma by using high-performance liquid chromatography (HPLC), which can be used for clinical drug concentration monitoring. The invention belongs to the technical field of drug analysis. Background technique

[0002] Ticagrelor, also known as Belinda, is a novel, oral, reversible platelet adenosine diphosphate (ADP) P2Y receptor antagonist. At the same time, the active metabolite AR-C124910XX can be rapidly generated after ticagrelor is absorbed. Both ticagrelor itself and its metabolites are active, and the activity of the drug itself is stronger than that of its metabolites. Therefore, it can rapidly Potently inhibits ADP-mediated platelet aggregation. As a platelet inhibitor, ticagrelor is widely used in patients with acute coronary syndrome, including those receiving drug therapy and percutaneous coronary intervention (PCI), to red...

Claims

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