Magnesium ion as antibacterial agent
A technology of magnesium ions and bacteria, applied in the direction of food ingredients as antimicrobial preservation, biocide, milk preservation, etc., can solve problems affecting the quality, function and safety of milk and its derivatives
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Embodiment 1
[0126] Magnesium ions inhibit biofilm formation
[0127] Different concentrations of Mg 2+ Effects of ions on biofilm formation. The results showed that Mg 2+ ions significantly inhibited the pellicle formation by Bacillus subtilis in a concentration-dependent manner ( Figure 1A ). Mg 2+ Ion suppression is not limited to MgCl 2 compounds because other magnesium salts such as MgSO 4 Also inhibits biofilm formation. This suggests that the inhibitory effect of magnesium salts is due to Mg2+ ion. Furthermore, at high concentrations of Mg 2+ Colony-type biofilm formation was also significantly inhibited in the presence of ions ( Figure 1A ).
[0128] Different concentrations of Mg were also examined 2+ Effect of ions on bacterial growth. The results showed that at the tested concentrations, Mg 2+ The presence of ions did not affect bacterial growth ( Figure 1B ).
[0129] Next, the effect of magnesium ions was visualized microscopically by testing the fasciculatio...
Embodiment 2
[0135] Mg 2+ , Ca 2+ and Na + Effects of ions on transcription of the operon responsible for substrate production
[0136] The skilled artisan will recognize that biofilm formation depends, at least in part, on the synthesis of extracellular matrix. The production of the extracellular matrix in Bacillus subtilis is regulated by two major operons: the epsA-O and tapA operons. The epsA-O operon is responsible for the production of exopolysaccharides, while the tapA operon is responsible for the production of amyloid-like fibrils.
[0137] Mg was examined by using transcriptional fusions of the promoters of epsA-O and tapA with the gene encoding β-galactosidase 2+ Effects of ions on matrix gene expression. Response to Mg 2+ With the addition of ions, the expression of the matrix operon was significantly reduced ( Figure 2A ). The decrease in eps expression was relatively small (approximately 4-fold) but significant, whereas at elevated concentrations of Mg 2+ The expres...
Embodiment 3
[0139] Magnesium ions inhibit biofilm formation in milk
[0140] The effect of magnesium ions on biofilm formation in milk was examined. The skilled person will recognize that the magnesium concentration in milk typically ranges between 4-6 mM. To examine the effect of magnesium ions, in the supplemented with MgCl 2 Bacteria were grown in milk to obtain milk with an increased concentration of magnesium ions of 1 mM, 3 mM or 5 mM. The results showed that Mg 2+ Ionic inhibition of biofilm formation by Bacillus subtilis in milk. Compared with the control, even if the magnesium concentration increased by 1mM, Mg 2+ The ion suppression effect is also remarkable. In addition, in MgCl 2 When the concentration increased by 5mM, the biofilm bundles almost completely disappeared ( Figure 3A ). Notably, with Figure 1B Similar to the results, bacterial growth was not significantly affected by Mg in milk 2+ The effect of increasing ion concentration ( Figure 3B ).
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