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Method for screening beta-lactamase antibiotic decomposing agent in dairy products by high flux matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)

A matrix-assisted laser and lactamase technology, which is applied in the direction of material analysis, measuring devices, and analytical materials through electromagnetic means, can solve the problems of inactivation of β-lactamase, etc., and achieve high-quality accuracy and good application Foreground, simple operation effect

Inactive Publication Date: 2011-04-27
SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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Problems solved by technology

The current research results show that residual β-lactamase can still be detected in commercially available finished fresh milk, which also shows that pasteurization cannot effectively inactivate β-lactamase, and eating these milk products is still risky

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  • Method for screening beta-lactamase antibiotic decomposing agent in dairy products by high flux matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)
  • Method for screening beta-lactamase antibiotic decomposing agent in dairy products by high flux matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)
  • Method for screening beta-lactamase antibiotic decomposing agent in dairy products by high flux matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS)

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[0044] The following specific implementation methods will help to understand the present invention, but do not limit the content of the present invention. A detection system for β-lactamase in milk was established using penicillin G as a substrate.

[0045] β-lactamase can open the β-lactam ring in penicillin (Penicillin G, PENG). For the MALDI-FTMS spectrum of penicillin G standard see figure 1 , the proton adduct peak of PENG (m / z 335.1) and the signal of potassium ion (m / z 373.1) can be observed in the figure, and the intensity of the proton adduct peak is the highest.

[0046] Since there is no standard product of penicilloic acid, it is necessary to convert PENG into PA through the catalysis of β-lactamase and then investigate its mass spectrometry behavior. The conversion of PENG to PA is shown in the mass spectrum as a decrease in the signal intensity of m / z 335.1 and an increase in the signal intensity of m / z 353.1. figure 2 The signal m / z 353.1 of the protonated p...

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Abstract

The invention relates to a method for detecting and screening a beta-lactamase antibiotic decomposing agent in dairy products by high flux matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS). The analytical method is based on the detection of the ring-opening reaction process of a beta-lactam ring containing substrate catalyzed by beta-lactamase, and the production of the ring-opening product is analyzed by MALDI-MS to reflect the amount of the beta-lactamase contained in the dairy products possibly, wherein the amount of the beta-lactamase is measured by the activity unit. In the method, the pretreatment step of a complex dairy product sample adopts a high-speed centrifugal method; the concentration of the beta-lactamase contained in the dairy products is reflected by the amount of the ring-opening product of the beta-lactamase substrate; and the hydrolysis ring-opening product of the beta-lactamase substrate is analyzed by MALDI-MS.

Description

technical field [0001] The invention relates to a high-throughput matrix-assisted laser desorption-mass spectrometry (MALDI-MS) detection and analysis method for β-lactamase inhibitors in milk products. technical background [0002] Food additives beautify the appearance of food, enrich the type and taste of food, and also closely affect people's lives. With the improvement of the quality of human life, people pay more and more attention to food safety, and the detection of food additive residues has become a necessary process stipulated by national laws. The Food Safety Law of the People’s Republic of China The article stipulates that "the production and operation of the following foods are prohibited: food produced with non-food raw materials or food with added chemical substances other than food additives and other substances that may endanger human health, or food produced with recycled food as raw materials (see "Food of the People's Republic of China") Article 28 of t...

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Application Information

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IPC IPC(8): G01N27/64G01N1/28
Inventor 郭寅龙许哲张立王昊阳张菁
Owner SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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