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Detection method and application of fshr gene-specific SNP markers, litter size traits of Turpan black sheep

A technology of litter size and labeling, which is applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, DNA/RNA fragments, etc.

Active Publication Date: 2022-04-26
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, there are few studies on the SNP markers of sheep litter size, and most of them focus on the research on the functional genes of BMP15, BMPR-IB, and GDF9; there is no report on the SNP markers affecting the litter size of Turpan black sheep

Method used

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  • Detection method and application of fshr gene-specific SNP markers, litter size traits of Turpan black sheep
  • Detection method and application of fshr gene-specific SNP markers, litter size traits of Turpan black sheep
  • Detection method and application of fshr gene-specific SNP markers, litter size traits of Turpan black sheep

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Embodiment 1

[0037] Reagents and equipment: magnetic stand (Life Technologies DynaMagTM-2);

[0038] Centrifuge (eppendorf Centrifuge 5424); Douglas Scientific NEXAR; Douglas Scientific Soellex; Douglas Scientific ARRAY; all provided by Biomike Biological Co., Ltd.;

[0039] Mix: KASP 2×Master Mix KBS-1016-011 1536 Formulation V4.0 (provided by LGC).

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Abstract

The invention discloses a FSHR gene-specific SNP marker, a method for detecting litter size traits of Turpan black sheep and an application thereof, and relates to the field of molecular breeding. The SNP marker of the present invention is derived from the Turpan black sheep FSHR gene, the SNP marker is located at position 75320741 on chromosome 3, the original base is C, and the variant base is T; the nucleotide sequence of the SNP marker is SEQ ID NO.1. The litter size of individuals with TT genotype at the SNP site was significantly higher than that of CC genotype. The nucleotide sequence of the primer of the present invention is SEQ ID NO.2-4. The present invention applies the above-mentioned SNP markers, primers and kits to the detection of the lambing traits of Turpan black sheep, and determines how the lambing traits of the black sheep to be tested are determined by detecting the SNP marker genotype; the above-mentioned SNPs, primers, kits and The detection method can be used in breeding to screen out varieties with more litters.

Description

technical field [0001] The invention relates to the technical field of molecular breeding, in particular to a FSHR gene-specific SNP marker, a detection method for litter size traits of Turpan black sheep, and an application thereof. Background technique [0002] Turpan black sheep, commonly known as "Tuokexun black sheep", is a small local breed of sheep. The adult ram weighs 46kg. It has the characteristics of fast growth and unique meat quality. It is an excellent local mutton sheep breed in Xinjiang. However, summer and winter in Xinjiang last nearly half a year. The highest temperature is as high as 42°C, and the coldest can reach minus 35 to minus 40°C. The natural conditions are harsh, and many fine varieties cannot survive here. The Turpan black sheep was formed under such a special condition through long-term natural and artificial selection. It not only adapts to the climate of the Turpan Basin, which is hot in summer and severe in winter; , and can rapidly increa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 刘武军马海玉方超刘玲玲王琼曹行吕士鹏于茜黄开飞蒋芳芳
Owner XINJIANG AGRI UNIV