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A screening method for microalgal cell wall mutants

A technology of microalgae cells and screening methods, which is applied in the field of cell screening, and can solve the problems of unrecorded screening methods and optimized conditions, and no application of lectin markers.

Active Publication Date: 2021-04-13
INST OF AQUATIC LIFE ACAD SINICA
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  • Description
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Problems solved by technology

However, there are no relevant reports on the application of lectin labeling to the screening of microalgae cell wall mutants, and the screening methods and optimization conditions have not been recorded.

Method used

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  • A screening method for microalgal cell wall mutants
  • A screening method for microalgal cell wall mutants
  • A screening method for microalgal cell wall mutants

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Embodiment Construction

[0054] In order to better explain the present invention and facilitate understanding, the present invention will be described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0055] Such as figure 1 Shown is a schematic flow chart of a screening scheme for cell wall mutants of microalgae, which is applicable to the screening of all cell wall mutants of unicellular microalgae. The basic process includes:

[0056] ① Analyze the type and abundance of monosaccharides in the cell wall of wild-type algae.

[0057] ② Determine the type of lectin according to the correspondence table between the abundant monosaccharides and the fluorescein-labeled lectins and the corresponding binding substrates.

[0058] ③ Staining pre-experiment, use the selected lectin combined with fluorescein to co-incubate with live algal cell populations, use flow cytometry to analyze the distribution of fluorescence intensity of algal cells, and explore the RCA t...

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Abstract

A screening method for cell wall mutants of microalgae, the method utilizes the specificity of lectins with fluorescent labels to label sugars on the surface of algal cells, and uses flow cytometer in conjunction to label lectins with specific traits. The algal cell wall mutants were screened out to obtain microalgal cell wall mutants. The present invention optimizes the key influencing factors and conditions of screening, thereby achieving higher screening efficiency and better repeatability, so as to screen characteristic algal species through genetic engineering means and biotechnology, and the development has more research and economic value microalgae such as Chlorella, Chlamydomonas and Haematococcus pluvialis.

Description

technical field [0001] The invention relates to the technical field of cell screening, in particular to a screening method for microalgae cell wall mutants. Background technique [0002] Microalgae have been widely used and studied because of their potential to produce a large number of high value-added compounds, in which the microalgae cell wall plays an important role in resisting stress and cell disruption. [0003] The rupture efficiency of microalgal cell walls seriously affects the yield of microalgal by-products. The cell walls of microalgae such as Chlamydomonas, Chlorella and Haematococcus are mainly composed of sugars. If certain sugars in the cell walls are changed, it may Changing the structure of the cell wall to make it thinner or more breakable improves harvest efficiency. Therefore, the screening of microalgae cell wall mutants with certain characteristics will be beneficial to the industrial application of microalgae. [0004] At present, a large number o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 韩丹翔林娟陈义胡强
Owner INST OF AQUATIC LIFE ACAD SINICA
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