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Microalgae cell wall mutant screening method

A technology of microalgal cells and screening method, which is applied in the field of cell screening, and can solve the problems of screening methods and optimization conditions that are not documented, and that the application of lectin markers has not been seen.

Active Publication Date: 2019-05-24
INST OF AQUATIC LIFE ACAD SINICA
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Problems solved by technology

However, there are no relevant reports on the application of lectin labeling to the screening of microalgae cell wall mutants, and the screening methods and optimization conditions have not been recorded.

Method used

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  • Microalgae cell wall mutant screening method
  • Microalgae cell wall mutant screening method
  • Microalgae cell wall mutant screening method

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Embodiment Construction

[0054] In order to better explain the present invention and facilitate understanding, the present invention will be described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0055] like figure 1 Shown is a schematic flow chart of a screening scheme for cell wall mutants of microalgae, which is applicable to the screening of all cell wall mutants of unicellular microalgae. The basic process includes:

[0056] ① Analyze the type and abundance of monosaccharides in the cell wall of wild-type algae.

[0057] ② Determine the type of lectin according to the correspondence table between the abundant monosaccharides and the fluorescein-labeled lectins and the corresponding binding substrates.

[0058] ③ Staining pre-experiment, use the selected lectin combined with fluorescein to co-incubate with live algal cell populations, use flow cytometry to analyze the distribution of fluorescence intensity of algal cells, and explore the RCA that...

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Abstract

The invention discloses a microalgae cell wall mutant screening method. Lectin with a fluorescent mark is used for specifically marking saccharides on algae cell surfaces, and lectin marked algae cellwall mutants with specific traits are screened by the aid of a flow cytometer to obtain microalgae cell wall mutants. Screening key influence factors and conditions are optimized, so that high screening efficiency and good repeatability are realized, characteristic algae filaments are screened by genetic engineering means and biotechnology, and microalgae such as chlorella, chlamydomonas and haematococcus pluvialis with more research and economic values are developed.

Description

technical field [0001] The invention relates to the technical field of cell screening, in particular to a screening method for microalgae cell wall mutants. Background technique [0002] Microalgae have been widely used and studied because of their potential to produce a large number of high value-added compounds, in which the microalgae cell wall plays an important role in resisting stress and cell disruption. [0003] The rupture efficiency of microalgal cell walls seriously affects the yield of microalgal by-products. The cell walls of microalgae such as Chlamydomonas, Chlorella and Haematococcus are mainly composed of sugars. If certain sugars in the cell walls are changed, it may Changing the structure of the cell wall to make it thinner or more breakable improves harvest efficiency. Therefore, the screening of microalgae cell wall mutants with certain characteristics will be beneficial to the industrial application of microalgae. [0004] At present, a large number o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 韩丹翔林娟陈义胡强
Owner INST OF AQUATIC LIFE ACAD SINICA
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