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Method for detecting various angiotensin converting enzyme inhibitor type hypotensive drugs in urine samples

A detection method and urine sample technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome detection, cumbersome detection, and large detection amount of prix-type antihypertensive drugs, and achieve simple detection methods and simple detection method, high precision

Inactive Publication Date: 2019-05-24
苏州旭辉检测有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the detection of pril-type antihypertensive drugs has always been cumbersome. The current detection method can only detect one antihypertensive drug at a time, resulting in a large amount of detection and cumbersome detection.

Method used

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  • Method for detecting various angiotensin converting enzyme inhibitor type hypotensive drugs in urine samples
  • Method for detecting various angiotensin converting enzyme inhibitor type hypotensive drugs in urine samples
  • Method for detecting various angiotensin converting enzyme inhibitor type hypotensive drugs in urine samples

Examples

Experimental program
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Effect test

Embodiment 1

[0068]Embodiment 1 provides a detection method for 8 kinds of pleil antihypertensive drugs in a urine sample, and the detection method is as follows:

[0069] (1) Pretreatment of urine samples

[0070] Standard solution preparation: benazepril / enalapril / imidapril / perindopril / ramipril / trandolapril / quinazepril / lisinopril were prepared using methanol under ultrasonic conditions Eight kinds of drugs were dissolved to a drug concentration of 2.5 mg / mL, and then diluted to 0.5 μg / mL with methanol, as a standard solution.

[0071] Blank control preparation: Take 10 μL of methanol and add it to 190 μL blank urine sample, vortex for 1 min to prepare a blank control sample, take 100 μL of blank sample, add 200 μL of 2% formic acid aqueous solution, vortex for 1 min, then add 800 μL Methanol, vortexed for about 1 min, and then centrifuged for 5 min at a centrifugation speed of 13,000 rpm and a centrifugation temperature of 4°C, and then took 200 μL of the supernatant and injected it as ...

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Abstract

The invention discloses a method for detecting various angiotensin converting enzyme inhibitor type hypotensive drugs in urine samples. The method is characterized in that the angiotensin converting enzyme inhibitor type hypotensive drugs in urine are detected by means of liquid chromatograph-mass spectrograph joint use. The method includes steps of urine sample pretreatment, liquid chromatograph-mass spectrograph parameter optimization and urine sample detection. The defect that a detection method in the prior art fails to simultaneously detect the various angiotensin converting enzyme inhibitor type hypotensive drugs in the urine samples is overcome, and the simple and convenience detection method is provided for detecting various angiotensin converting enzyme inhibitor type hypotensivedrugs in the urine samples. The detection method is convenient and simple in operation and high in accuracy, and whether the various angiotensin converting enzyme inhibitor type hypotensive drugs exist in urine or not can be judged simultaneously.

Description

technical field [0001] The invention relates to the field of urine sample detection, in particular to a method for detecting multiple pril-type antihypertensive drugs in a urine sample. Background technique [0002] Primid is an abbreviation for angiotensin-converting enzyme inhibitors (ACEI). Angiotensin II has strong physiological activities, including: 1. Direct contraction of vascular smooth muscle; 2. Stimulation of the adrenal cortex to secrete aldosterone, which increases water and sodium retention; 3. Maintenance of sympathetic nerve activity. [0003] Antihypertensive drugs mainly act on the renin-angiotensin-aldosterone system (RAA system). Inhibit the angiotensin-converting enzyme (ACEI) of the RAA system, prevent the conversion of angiotensin Ⅰ or angiotensin Ⅱ, and can inhibit the secretion of aldosterone and reduce water and sodium retention. It has obvious antihypertensive effect on various types of hypertension, and can improve the heart function of patient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 李小川张枢黄晓林李玉杰王林花刘健袁小青聂巧刘达石海兵
Owner 苏州旭辉检测有限公司
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