Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for harvesting isochrysis galbana and achieving semi-continuous culture by means of ethyl alcohol concentration

A semi-continuous technology of Isoflagellates, applied in the field of microalgae biology, can solve the problems of flocculant residue, flocculant toxicity, cumbersome operation, and high cost, and achieve the effects of easy operation, simple collection, and low volatility

Active Publication Date: 2019-05-28
SHANGHAI OCEAN UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the problems of high cost, cumbersome operation (centrifugation method and membrane filtration method), and problems such as flocculant residue and flocculant toxicity (flocculant method) existing in the traditional dinoflagellate harvesting method , on the basis of repeated tests and screening of various substances, a method for harvesting Isoflagellates with ethanol and realizing semi-continuous culture is provided

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for harvesting isochrysis galbana and achieving semi-continuous culture by means of ethyl alcohol concentration
  • Method for harvesting isochrysis galbana and achieving semi-continuous culture by means of ethyl alcohol concentration
  • Method for harvesting isochrysis galbana and achieving semi-continuous culture by means of ethyl alcohol concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] Cultivate Isochrysis globosa with F / 2 culture medium until the relative growth decline period, to be harvested.

[0024] Among them, the F / 2 culture medium for cultivating Isochrysis globosa is: adding 74.8mg NaNO per liter of seawater 3 , 4.4mgNaH 2 PO 4 , 12mg Na 2 SiO 3 9H 2 O, 1mL trace element solution, 1mL vitamin solution. Trace element solution: 23mgZnSO 4 4H 2 O, 7.3 mg Na 2 MoO 4 2H 2 O, 17.8 mg MnCl 2 4H 2 O, 12 mg CoCl 2 ·6H 2 O, 10 mg CuSO 4 ·5H 2 O, 4.35 g Na 2 EDTA, 3.9 g FeC 6 h 5 o 7 ·5H 2 O, 1000mL distilled water. Vitamin solution: 0.5 mg vitamin B 12 , 0.5mg Biotin, 100mg Vitamin B 1 , 1000mL distilled water.

[0025] Among them, the inoculation density of Isochrysis globosa was 1×10 6 cell / mL, at a temperature of 25°C and a light intensity of 70μmol / m 2 s, photoperiod 16L: 6 days under the condition of 8D, algae cell density reaches 6.0×10 6 cell / mL, ready to harvest.

[0026] Take 50mL of the above-mentioned algae liquid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of microalgae organisms, in particular to a method for harvesting isochrysis galbana and achieving semi-continuous culture by means of ethyl alcohol concentration. Ethyl alcohol is added into liquid of the cultured and to-be-harvested isochrysis galbana, after standing is conducted for 18-24 hours, siphonage is used for harvesting cells of the isochrysis galbana which precipitates on the bottom of a container, and meanwhile, new culture liquid is added into the residual isochrysis galbana liquid for secondary aerated culture, so that semi-continuousculture of the isochrysis galbana is achieved. The ethyl alcohol in an extremely low concentration is used for treating the isochrysis galbana to make the isochrysis galbana lose athletic ability, thus a precipitation method can be used for harvesting most of the isochrysis galbana cells in the isochrysis galbana liquid, the harvesting rate is over 70%, the added ethyl alcohol is low in concentration and easy to volatilize, the harvested microalgae cells are safe and non-toxic, the re-suspension performance is good, the ethyl alcohol cost is very low, and the residual isochrysis galbana cellsin the isochrysis galbana liquid subjected to isochrysis galbana cell precipitation and siphonage harvesting can continue to proliferate under an aerated condition when supplemented with the new culture liquid, so that semi-continuous culture of the isochrysis galbana is achieved.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method for concentrating and harvesting Isoflagellates by using ethanol and realizing semi-continuous culture. Background technique [0002] Isochrysis galbana (Isochrysis galbana), Chrysophyceae, Isochrysis galbana. Single-cell motile individual, variable in shape, oval in shape, without cell wall, with two equal-length flagella. Because the individual is small, usually 4-7 microns long, 2.7-4.4 microns wide, 2.4-3.0 microns thick, no cell wall, easy to digest, rich in polysaccharides, carotene and high-energy lipids, it is aquaculture around the world (especially It is a widely used bait microalgae in shellfish farming). Isochrysis globosa is also a natural treasure house of physiologically active substances, among which β-carotene and highly unsaturated fatty acids such as EPA and DHA have the functions of anti-aging, anti-stress and promoting the development of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 黄旭雄薛毓才
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com