Method for preparing tobacco flavors and fragrances by using cordyceps sinensis or tremella aurantialba and flowers
A technology of Cordyceps sinensis and tobacco flavor, which is applied in the direction of essential oil/fragrance, preparation of tobacco, tobacco, etc. It can solve the problems of unpleasant smell and increase the difficulty of tobacco flavor and fragrance, and achieve low cost, high immune effect and simple process Effect
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Embodiment 1
[0057] Embodiment 1: Cordyceps sinensis+Sophora japonica
[0058] Specific steps are as follows:
[0059] (1) Take 80mL seed culture medium and place it in a 250mL shake flask, take a piece of 1cm 2 The Cordyceps sinensis stored at 4°C was inoculated into the seed medium, and the -1 Cultivate for 5 days under the condition of , and obtain the seed solution;
[0060] (2) Take 100g of fermentation medium (sterilized at 105°C for 20min) and place it in a 250mL shake flask, inoculate the seed liquid into the fermentation medium according to the parameters in Table 2 for fermentation, and obtain fermentation product A 0 、A 1 、A 2 、A 3 ; where, A 0 group is the blank control group.
[0061] With 2-octanol as the internal standard (final concentration 8.2ppb), the fermentation product A 0 、A 1 、A 2 、A 3 Analyze according to the analysis method recorded in the flavor and fragrance solid-phase microextraction-gas analysis method, wherein, the fermented product A 0 、A 1 The...
Embodiment 2
[0078] Example 2: golden ear + chrysanthemum
[0079] Specific steps are as follows:
[0080] (1) Take 80mL seed culture medium and place it in a 250mL shake flask, take a piece of 1cm 2 The golden ear stored at 4°C was inoculated into the seed medium, and the -1 Cultivate for 5 days under the condition of , and obtain the seed solution;
[0081] (2) Take 100g of fermentation medium (sterilized at 105°C for 20min) and place it in a 250mL shake flask, inoculate the seed liquid into the fermentation medium according to the parameters in Table 6 for fermentation, and obtain fermentation product B 0 , B 1 , B 2 , B 3 ; where, B 0 group is the blank control group.
[0082] ferment B 0 , B 1 , B 2 , B 3 Analyze according to the analysis method recorded in the flavor and fragrance solid phase microextraction-gas analysis method, wherein, the fermented product B 0 , B 1 The analysis results are shown in Table 7-8 and Figure 3-4 .
[0083] For ferment B 0 , B 1 , B 2 ,...
Embodiment 3
[0100] Embodiment 3: Cordyceps + osmanthus
[0101] Specific steps are as follows:
[0102] Sophora pagoda in embodiment 1 is replaced with sweet-scented osmanthus, repeats A in embodiment 1 1 Group experiment, get fermented product A 4 . For the fermented product A obtained 4 Smell the fragrance, and the results are shown in Table 10.
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