Acidified hardened soil restoring bacterial agent as well as preparation method and application method thereof
A technology of soil remediation and bacterial agent, applied in the field of acidified compacted soil remediation bacterial agent and its preparation, can solve problems such as degradation, restriction of soil production potential, soil acidified compaction fertility, etc., to improve buffer capacity, promote health and sustainable use , The effect of simple process
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[0022] Some embodiments of the present invention also provide a preparation method of the above-mentioned acidified and compacted soil remediation bacterial agent, which includes: microbial bacterial agent, oyster shell powder, nano-microelectric energy materials, mineral humic acid, amino acids, enzymatic alginic acid and Anionic polyacrylamide mixed evenly.
[0023] Further, in some embodiments, the preparation of the microbial inoculum comprises the following steps: inoculating the strains of the microbial inoculum into the medium for liquid fermentation culture, preferably, the inoculation amount is 0.1-0.3%; fermenting the collected The solution is mixed with diatomaceous earth for adsorption, dried and pulverized. Wherein, the mass ratio of diatomaceous earth to fermentation broth may be 7-9:1, preferably 8:1.
[0024] Specifically, in some embodiments, the carbon source of the culture medium includes at least one of sucrose, starch, glucose and lactose. Preferably at ...
Embodiment 1
[0045] The preparation method of a kind of acidified compacted soil remediation bacterial agent provided by the present embodiment comprises the following steps:
[0046] 1. Select starch as the carbon source, yeast powder as the nitrogen source, the carbon-to-nitrogen ratio of the medium is 15:1, the liquid volume is 50%, inoculate Bacillus licheniformis seeds, the inoculum volume is 0.2%, and the initial pH value of fermentation is 7.2, at 39°C Fermented under the condition for 36 hours to obtain the fermented liquid;
[0047] 2. The collected fermented liquid and the pre-configured diatomite are mixed and adsorbed according to the mass ratio of bacteria liquid: diatomite = 1:8, and after the adsorption, they are dried and pulverized to make a composite microbial bacterial agent;
[0048] 3. After the kelp is dried in the sun and cleaned of impurities, it is dried at 80 degrees, crushed, and passed through an 80-mesh sieve to obtain seaweed powder. The seaweed powder is degr...
Embodiment 2
[0051] The preparation method of a kind of acidified compacted soil remediation bacterial agent provided by the present embodiment comprises the following steps:
[0052] 1. Select starch as the carbon source, yeast powder as the nitrogen source, the carbon-to-nitrogen ratio of the medium is 10:1, the liquid volume is 50%, inoculate Bacillus licheniformis seeds, the inoculum volume is 0.2%, and the initial pH value of fermentation is 7.2, at 39°C Fermented under the condition for 36 hours to obtain the fermented liquid;
[0053] 2. The collected fermented liquid and the pre-configured diatomite are mixed and adsorbed according to the mass ratio of bacteria liquid: diatomite = 1:8, and after the adsorption, they are dried and pulverized to make a composite microbial bacterial agent;
[0054] 3. After the sargassum is dried in the sun and cleaned of impurities, it is dried at 80 degrees, crushed, and passed through a 80-mesh sieve to obtain seaweed powder. The seaweed powder is ...
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