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Method for constructing dengue model

A construction method and technology for dengue fever, applied in the field of biological model construction, can solve problems such as difficulty in genome manipulation, restricted infection models, and research limitations

Inactive Publication Date: 2019-06-14
GUANGDONG GENERAL HOSPITAL
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Problems solved by technology

The prevention and control of dengue depends on the important progress of DENV basic research, but the basic research of DENV is limited by the infection model
[0003] At present, the DENV insect infection model is mainly Aedes aegypti, because the genetic background of the mosquito is not clear and the genome manipulation is difficult, which limits the research

Method used

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  • Method for constructing dengue model
  • Method for constructing dengue model
  • Method for constructing dengue model

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Embodiment Construction

[0018] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0019] DENV II 43 can efficiently infect Drosophila melanogaster S2 cells

[0020] Method: the prior art discloses that DENV is difficult to infect Drosophila cells. The present invention adopts the C6 / 36 cell culture of Aedes albopictus to obtain 43 strains of DENV virus type II, and concentrates and infects Drosophila melanogaster S2 cells (embryonic cells). Then immunofluorescence was used to analyze the ratio of positive expression of DENV NS1 protein in S2 cells.

[0021] Such as figure 1 As shown, 10moi DENV can effectively infect S2 cells; after 5 days of infection analysis, it is found that the infection rate can reach more than 50%; this shows that DENV II type 43 strains can more effectively infect Drosophila melanogaster S2 cells, w...

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Abstract

The invention discloses a method for constructing a dengue model. The organism of the dengue is drosophila melanogaster or drosophila melanogaster embryo cells, and a dengue virus is injected into thedrosophila melanogaster or the drosophila melanogaster embryo cells; the dengue virus is obtained by culture of C6 / 36 cells of aedes albopictus. The DENV-infected drosophila melanogaster model is constructed for the first time, can better simulate DENV-infected mosquitoes and humans, and can provide a good theoretical basis for DENV in insect and human infection mechanism and antiviral drug treatment research.

Description

technical field [0001] The invention belongs to the technical field of biological model construction, in particular to a method for constructing a dengue fever model. Background technique [0002] Dengue fever is a mosquito-borne viral infection that causes flu-like symptoms; sometimes it develops into a potentially fatal complication called severe dengue. The global incidence of dengue fever has increased substantially in recent decades, and about half of the world's population is now at risk of dengue fever. Severe dengue is a leading cause of severe morbidity and death in children in several countries in Asia and Latin America. The prevention and control of dengue depends on the important progress of DENV basic research, but the basic research of DENV is limited by the infection model. [0003] At present, the DENV insect infection model is mainly Aedes aegypti, because the genetic background of the mosquito is not clear and the genome manipulation is difficult, which l...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N5/07A01K67/033C12R1/93
Inventor 彭涛陈文莉蔡华陈笛肖静
Owner GUANGDONG GENERAL HOSPITAL