Application and product of dsRNA in potato beetle control

A potato beetle and insecticide technology, applied in the direction of DNA/RNA fragments, application, recombinant DNA technology, etc., can solve the problems of instability, high production cost, immature dsRNA technology, etc., achieve low cost, fast action process, Effective and inexpensive Specific effect

Pending Publication Date: 2019-06-21
INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current RNAi technology still has obvious shortcomings: ① The dsRNA or siRNA that triggers RNAi is easily degraded by nucleases u

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application and product of dsRNA in potato beetle control
  • Application and product of dsRNA in potato beetle control
  • Application and product of dsRNA in potato beetle control

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Specific embodiment 1: The application of dsLdRpt3 in potato beetle control in this embodiment, wherein the nucleotide sequence of dsLdRpt3 is shown in SEQ ID NO:1.

Example Embodiment

[0031] Embodiment 2: The difference between this embodiment and the first embodiment is that dsLdRpt3 is used as the active ingredient of the potato beetle insecticide. Others are the same as the first embodiment.

Example Embodiment

[0032] Specific embodiment three: The difference between this embodiment and specific embodiment one or two is that the bacterial liquid containing the transgenic engineered bacteria expressing dsLdRpt3 is used as the potato beetle insecticide. Others are the same as the first or second embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an application and a product of dsRNA in potato beetle control, and relates to an application of a gene product in potato beetle control and a derivative product. The dsRNA product has strong stability, mature production technology and low cost, and is an effective cheap 'gene pesticide' product with strong specificity, green and environmental protection. The application ofthe dsRNA product in potato beetle control has great and far-reaching significance in potato beetle control. After a potato beetle pesticide is sprayed on leaves of potato plants at a low dose, the potted potato plants can continuously resist insects for more than 28 days, and the control effect reaches more than 80%; and under the outdoor strong ultraviolet condition, the potato beetle pesticidestill has a strong poisoning effect on potato beetles, the field prevention and control lasting period is about 3 weeks, and the prevention and control effect is gradually reduced from 50% to about 10%.

Description

technical field [0001] The invention relates to the application of a gene product in the control of potato beetle and its derivative product. Background technique [0002] Potato is an important grain, vegetable, feed crop and industrial raw material crop in the world. In recent years, due to its obvious comparative economic benefits and other characteristics, the planting area in my country has continued to increase, and it has become the first in my country after rice, wheat and corn. 4 major staple food crops. The potato beetle, Leptinotarsa ​​decemlineata, also known as the potato beetle, its English name Colorado Potato Beetle (CPB), belongs to the order Coleoptera and the family Chrysolithidae. It is recognized as a destructive pest of potatoes in the world, and it is also one of the major quarantine targets in my country. Its host range is relatively narrow, and it mainly harms more than 20 species of Solanaceae plants such as potatoes, eggplants, cultivated tomatoes,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/113C12N15/70C12N1/21A01N57/16A01P7/04C12R1/19
Inventor 吐尔逊·阿合买提付开赟米热古丽·胡尔西达何江郭文超李超付文君于建新王燕陈瑞刚
Owner INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products