Method and detection reagent for detecting plasma concentration of imatinib mesylate in blood plasma with LC-MS/MS (liquid chromatography-tandem mass spectrometry)

A technology of imatinib mesylate and liquid chromatography, which is applied in the field of detection of imatinib mesylate blood drug concentration, can solve problems such as cross-reaction, interference detection, and difficulty in accurate identification, achieving small impact, The effect of high recovery rate and precision

Inactive Publication Date: 2019-06-28
茆勇
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Problems solved by technology

At present, the detection techniques commonly used in clinical testing are mainly based on immunoassay methods, but immunoassays have inherent defects in the detection of small molecule compounds
Even with monoclonal antibodies, it is difficult for these antibodies to accurately recogni

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  • Method and detection reagent for detecting plasma concentration of imatinib mesylate in blood plasma with LC-MS/MS (liquid chromatography-tandem mass spectrometry)
  • Method and detection reagent for detecting plasma concentration of imatinib mesylate in blood plasma with LC-MS/MS (liquid chromatography-tandem mass spectrometry)
  • Method and detection reagent for detecting plasma concentration of imatinib mesylate in blood plasma with LC-MS/MS (liquid chromatography-tandem mass spectrometry)

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Embodiment 1

[0044] A method for liquid chromatography tandem mass spectrometry detection of imatinib mesylate blood drug concentration in plasma, comprising the following steps:

[0045] (i) Preparation of standard solution

[0046] Weigh 10mg of imatinib mesylate standard substance, put it in a 10ml volumetric flask, add appropriate amount of methanol to dissolve it, make it to volume, shake well, and obtain a standard substance stock solution with a concentration of 1mg / ml, and store it in a refrigerator at 4°C.

[0047] (ii) Preparation of internal standard solution

[0048] Weigh 10mg of carbamazepine, put it in a 10ml volumetric flask, add an appropriate amount of methanol to dissolve and make up to volume, and shake well to obtain an internal standard stock solution with a concentration of 1mg / ml, and store it in a refrigerator at 4°C. It was diluted with methanol to 500ng / ml before the test.

[0049] (iii) Sample pretreatment

[0050] Use a 200ul pipette to precisely draw 200ul ...

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Abstract

The invention discloses a method and a detection reagent for detecting plasma concentration of imatinib mesylate in blood plasma with LC-MS/MS (liquid chromatography-tandem mass spectrometry). The method comprises steps as follows: (i), preparing a standard solution; (ii), preparing an internal standard solution; (iii), pre-processing samples; (iv), drawing a standard curve; (v), measuring the plasma concentration of imatinib mesylate in blood plasma. The detection reagent comprises a moving phase A, a moving phase B, the standard solution and the internal standard solution. The method for detecting the plasma concentration of imatinib mesylate in blood plasma with LC-MS/MS is provided, imatinib and carbamazepine as an internal standard substance can be completely separated with the method, besides, the method is slightly affected by an endogenous substance impurity peak, and recovery rate and precision are high.

Description

technical field [0001] The invention belongs to a method for detecting blood drug concentration of imatinib mesylate, and in particular relates to a method for detecting blood drug concentration of imatinib mesylate in blood plasma by liquid chromatography tandem mass spectrometry and a detection reagent. Background technique [0002] Imatinib mesylate (imatinib) is the first generation of small molecule tyrosine kinase inhibitors. Imatinib mesylate is a small-molecule targeted drug targeting the pathogenesis of chronic myeloid leukemia (CML), and is also recommended as a first-line drug for the treatment of metastatic, recurrent, unresectable, and other gastrointestinal stromal tumors (GIST) and postoperative adjuvant medication, but the efficacy and toxicity of the drug vary greatly among individuals, such as gender, drug metabolism, etc., and are easily affected by other drugs. lifetime. At present, the detection techniques commonly used in clinical testing are mainly b...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 茆勇华东盛青松辛君伟
Owner 茆勇
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