Nano antibody of Zika virus, and preparation method and application thereof

A technology of Zika virus and nano-antibody, which is applied in chemical instruments and methods, anti-viral immunoglobulin, and the use of vectors to introduce foreign genetic material, etc. It can solve the problems of sensitivity and other problems, and achieve the effect of strong specificity and high affinity
CN109970853APending Publication Date: 2019-07-05BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN ¡ China
Current Assignee / Owner
BLOOD TRASFUSION INST CHINESE ACAD OF MEDICAL SCI
Publication Date
2019-07-05

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Abstract

The invention relates to the technical field of biological nano materials, in particular to a nano antibody of Zika virus, and a preparation method and application thereof. An alpaca is immunized withvirus particles of Zika virus and then cloned; cloned DNA sequences are inserted in appropriate positions of a phage coat protein structural gene, and a sequence with higher affinity is selected through affinity elutriation; finally, monoclonal ELISA (enzyme-linked immunosorbent assay) identification is carried out, and antibody expression and purification can be carried out to obtain the nano antibody of Zika virus. The nano antibody of Zika virus is obtained by immunizing the alpaca and through a phage display technology, and thus the nano antibody can be highly expressed in prokaryotic oreukaryotic systems, has strong specificity and high affinity, and is more suitable for specific serological detection of Zika virus.
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Description

technical field

[0001] The invention relates to the technical field of biological nanomaterials, in particular to a Zika virus nanobody and its preparation method and application. Background technique

[0002] The serological detection of Zika virus can use the antibody detection of Zika virus. However, the antibodies currently on the market are generally obtained by immunizing mice or rabbits (polyclonal antibodies), or monoclonal antibodies obtained by hybridoma technology. These antibodies have the characteristics of large molecular weight and weak affinity. Contents of the invention

[0003] The present invention aims to provide a Zika virus nanobody and a preparation method thereof to solve the sensitivity problem of Zika virus enzyme-linked immunoassay detection, so as to realize the serological detection of Zika virus in blood donors.

[0004] In order to achieve the above object, the technical scheme adopted in the present invention is:

[0005] The preparation m...

Claims

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