Application of dendrobium polysaccharide in preparation of drugs to prevent or restore reproductive injury after chemotherapy

A technology of dendrobium polysaccharides and medicines, which is applied in the application field of dendrobium polysaccharides to prepare drugs for preventing or recovering reproductive damage after nitrogen mustard anticancer drug chemotherapy, can solve the problems of prevention and repair blanks, and achieve a simple and easy preparation method Effect

Inactive Publication Date: 2019-07-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The high incidence of infertility in China has climbed to the level of Western countries in the past ten years (accounting for 20% of the reproductive population), and the reproductive damage caused by cancer radiotherapy and chemotherapy has become increasingly prominent, and the interaction between Dendrobium and its polysaccharides and reproductive function Whether it has any effect on the prevention and repair of reproductive damage is still almost blank, especially the prevention and rehabilitation of reproductive function damage caused by radiotherapy and chemotherapy has not been reported.

Method used

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  • Application of dendrobium polysaccharide in preparation of drugs to prevent or restore reproductive injury after chemotherapy
  • Application of dendrobium polysaccharide in preparation of drugs to prevent or restore reproductive injury after chemotherapy
  • Application of dendrobium polysaccharide in preparation of drugs to prevent or restore reproductive injury after chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Extraction and Detection of Dendrobium Polysaccharides

[0052] 1. Extraction of Dendrobium polysaccharides

[0053] Get Dendrobium candidum (Dendrobium candidum is planted in the experimental base of the Institute of Crops of Guangdong Academy of Agricultural Sciences, is identified as Dendrobium candidum through species identification, fresh strips are sampled and then dried and crushed to obtain Dendrobium candidum dry powder) 4g, according to the material of 1:30 (m / V) Add distilled water to the liquid ratio, fully dissolve, extract three times in a 90°C water bath, 120 minutes each time, divide the extract into 7mL centrifuge tubes, freeze and solidify in a -20°C refrigerator, and then freeze in a freeze dryer at 10000r / Centrifuge for 6 hours, and concentrate the liquid components in the tube to concentrate the extract. Dissolve and collect the concentrated product with 1-2 mL of distilled water, then add 4 times the volume of absolute ethanol, precipit...

Embodiment 2

[0062] Changes in the sperm and testis-related parameters of embodiment 2 model mice

[0063] Cyclophosphamide injection is produced by Shanxi Pode Pharmaceutical Co., Ltd., specification: 0.2g cyclophosphamide / bottle, National Pharmaceutical Standard: H20040813.

[0064] 1. Solution preparation

[0065] (1) Preparation of dendrobium polysaccharide solution: take the dendrobium polysaccharide powder prepared in Example 1, dissolve it in distilled water, and prepare a 100 mg / mL dendrobium polysaccharide solution.

[0066] (2) Preparation of cyclophosphamide injection: Take one cyclophosphamide for injection and add 10 mL of normal saline to prepare 20 mg / mL cyclophosphamide injection.

[0067] (3) Preparation of HTF solution (human fallopian tube culture fluid): prepare HTF solution according to the formula in Table 1.

[0068] Table 1 HTF solution formula

[0069]

[0070]

[0071] 2. Animal testing

[0072] (1) Blank control group (from day 0 of the experiment, norm...

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Abstract

The invention discloses an application of dendrobium polysaccharide in preparation of drugs to prevent or restore reproductive injury after chemotherapy. The dendrobium polysaccharide applied to prevent and treat male reproductive injury caused by radiotherapy and chemotherapy has obvious therapeutic effect and small side effects, and can effectively reduce the damage caused by nitrogen mustard anticancer drugs to the reproductive system, which not only makes the sperm quantity of mice rise significantly, but also improves the sperm quality, and effectively improves the antioxidant activity ofmouse testicular tissue, and in mouse testicular tissue, the level of SOD, GPx, GSH and CAT is obviously increased while the level of MDA is obviously decreased. The dendrobium polysaccharide with wide source of raw materials is simple in extraction and purification method, high in sugar content and suitable for large-scale production and popularization and has a good prospect in the preparationand/or treatment of reproductive injury after chemotherapy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of dendrobium polysaccharides in the preparation of drugs for preventing or recovering reproductive damage after chemotherapy with nitrogen mustard anticancer drugs. Background technique [0002] In today's society, the incidence of cancer is increasing year by year. In 2012, the number of cancer diagnoses in my country reached 3.5862 million, and 2.1866 million people died of various cancers; by 2015, the number of cancer diagnoses had risen to 4.292 million, while the death toll had risen to 284.1 million people. The top ten malignant tumors leading to death in my country are gastric cancer, liver cancer, esophageal cancer, colorectal cancer, leukemia and lymphoma, cervical cancer, nasopharyngeal cancer, and breast cancer. threaten. Cyclophosphamide is the first-line drug for the clinical treatment of cancer. It is widely used in the treatment of cancer and a variet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P15/00A61P15/08
CPCA61K31/715
Inventor 芦春斌张裕明林泽斌朱倍倍仇平乐
Owner JINAN UNIVERSITY
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