Application of dendrobium polysaccharide in preparation of drugs to prevent or restore reproductive injury after chemotherapy
A technology of dendrobium polysaccharides and medicines, which is applied in the application field of dendrobium polysaccharides to prepare drugs for preventing or recovering reproductive damage after nitrogen mustard anticancer drug chemotherapy, can solve the problems of prevention and repair blanks, and achieve a simple and easy preparation method Effect
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Embodiment 1
[0051] Example 1 Extraction and Detection of Dendrobium Polysaccharides
[0052] 1. Extraction of Dendrobium polysaccharides
[0053] Get Dendrobium candidum (Dendrobium candidum is planted in the experimental base of the Institute of Crops of Guangdong Academy of Agricultural Sciences, is identified as Dendrobium candidum through species identification, fresh strips are sampled and then dried and crushed to obtain Dendrobium candidum dry powder) 4g, according to the material of 1:30 (m / V) Add distilled water to the liquid ratio, fully dissolve, extract three times in a 90°C water bath, 120 minutes each time, divide the extract into 7mL centrifuge tubes, freeze and solidify in a -20°C refrigerator, and then freeze in a freeze dryer at 10000r / Centrifuge for 6 hours, and concentrate the liquid components in the tube to concentrate the extract. Dissolve and collect the concentrated product with 1-2 mL of distilled water, then add 4 times the volume of absolute ethanol, precipit...
Embodiment 2
[0062] Changes in the sperm and testis-related parameters of embodiment 2 model mice
[0063] Cyclophosphamide injection is produced by Shanxi Pode Pharmaceutical Co., Ltd., specification: 0.2g cyclophosphamide / bottle, National Pharmaceutical Standard: H20040813.
[0064] 1. Solution preparation
[0065] (1) Preparation of dendrobium polysaccharide solution: take the dendrobium polysaccharide powder prepared in Example 1, dissolve it in distilled water, and prepare a 100 mg / mL dendrobium polysaccharide solution.
[0066] (2) Preparation of cyclophosphamide injection: Take one cyclophosphamide for injection and add 10 mL of normal saline to prepare 20 mg / mL cyclophosphamide injection.
[0067] (3) Preparation of HTF solution (human fallopian tube culture fluid): prepare HTF solution according to the formula in Table 1.
[0068] Table 1 HTF solution formula
[0069]
[0070]
[0071] 2. Animal testing
[0072] (1) Blank control group (from day 0 of the experiment, norm...
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