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Method for establishing mouse model of heart failure with preserved ejection fraction

A technology of ejection fraction and mouse model, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve problems such as difficult to simulate the hemodynamic characteristics of HFpEF, and achieve a good application prospect Effect

Active Publication Date: 2019-07-23
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of appropriate HFpEF animal models, the research on the treatment of its therapeutic drugs is very limited. The difficulty in preparing HFpEF animal models is to replicate the clinical characteristics of HFpEF animal models, especially it is difficult to simulate the hemodynamic characteristics of HFpEF

Method used

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  • Method for establishing mouse model of heart failure with preserved ejection fraction
  • Method for establishing mouse model of heart failure with preserved ejection fraction
  • Method for establishing mouse model of heart failure with preserved ejection fraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Establishment of the mouse model of heart failure with preserved ejection fraction of the present invention

[0025] 1 Experiment purpose

[0026] A mouse model of heart failure with preserved ejection fraction (that is, a mouse model of heart failure with normal ejection fraction) was established.

[0027] 2 Materials and methods

[0028] 2.1 Experimental materials

[0029] 2.1.1 Experimental animals

[0030] Normal control mice and mice with mitochondrial dysfunction (SITR3-KO, NDUFS4-KO) were selected as experimental subjects, half male and half female, free to eat and drink, 12 hours of light / dark, room temperature controlled at 22±1°C. Mitochondrial dysfunction (SITR3-KO, NDUFS4-KO) mice were purchased from Jackson Laboratory. All animal experiments and handling were approved by the Animal Care and Use Committee of West China Hospital, Sichuan University.

[0031] 2.1.2 Experimental drugs and reagents

[0032] 1) D-glucose (Sigma, USA)

[0033] 2) ...

Embodiment 2

[0105] Example 2 Use the model of the present invention to screen the drug for the treatment of heart failure with preserved ejection fraction

[0106] a. According to the method of Example 1, a mouse model of heart failure with preserved ejection fraction was established;

[0107] b. administering the drug candidate to an animal model;

[0108] c. Observe the effect of candidate drugs on various indicators of heart failure with preserved ejection fraction, and evaluate potential drugs for treating heart failure with preserved ejection fraction.

[0109] To sum up, the present invention has successfully constructed a mouse and rat model of heart failure with preserved ejection fraction, which can be used for research on heart failure with preserved ejection fraction, and has a good application prospect.

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Abstract

The present invention relates to a preparation method of a mouse model of heart failure with preserved ejection fraction. The preparation method comprises the following steps: mice are taken and the taken mice are administered with a high-fat diet and deoxycorticosterone pivalate. The mouse model of the heart failure with the preserved ejection fraction is successfully constructed, can be used forresearch of the heart failure with the preserved ejection fraction and has excellent application prospects.

Description

technical field [0001] The invention relates to the establishment of a mouse model of heart failure with preserved ejection fraction. Background technique [0002] Heart failure is one of the leading causes of death and disability worldwide. According to the different ejection fraction, heart failure is divided into two types, namely heart failure with reduced ejection fraction (HFREF) and heart failure with normal ejection fraction (HFPEF). . HFREF is the ejection fraction (EF) ≤ 40%, and HFPEF is the ejection fraction (EF ≥ 50%), accompanied by symptoms of heart failure such as pulmonary congestion, dyspnea and reduced exercise tolerance. The main characteristic of HFpEF is decreased left ventricular diastolic function, which may be related to myocardial fibrosis, myocardial hypertrophy, ventricular wall stiffness, vascular endothelial dysfunction, and decreased cardiovascular reserve function. HFpEF patients are generally elderly, mostly women, and the main risk factors...

Claims

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Application Information

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IPC IPC(8): A61K31/573A61K49/00A01K67/02
CPCA01K67/02A61K31/573A61K49/0008
Inventor 李涛邓艳田蓉
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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