Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of composite microbial preparation and its application

A technology of compound microorganisms and microorganisms, applied in the field of microorganisms, can solve the problems of high cost, weak effect of disease control, time-consuming and labor-intensive

Active Publication Date: 2020-10-27
漳州三炬生物技术有限公司 +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the defects of high cost of decomposed straw, time-consuming and laborious, and weak effect of disease prevention and control, the present invention provides a green and pollution-free compound microbial preparation, which has dual functions of pesticide and fertilizer, can effectively reduce the frequency of fertilization, reduce the Pesticide use cost, friendly to the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of composite microbial preparation and its application
  • A kind of composite microbial preparation and its application
  • A kind of composite microbial preparation and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Raw material crushing: fully crush the straw below 80-100 mesh, and mix it evenly for full fermentation;

[0051] 2. Cultivate Bacillus amyloliquefaciens Sanju-17 (preservation number CGMCC NO.15403) with beef extract medium, ferment at 22-28°C, and ferment for 2 days to obtain Bacillus amyloliquefaciens with a viable count of 450 million / g Sanju-17 fermentation broth;

[0052] The jelly-like Bacillus Sanju 01 strain (preservation number CGMCC NO.3995) was cultivated in nitrogen-free medium, the fermentation temperature was 22-28°C, and the fermentation time was 2 days to obtain jelly-like spores with a viable count of 200 million / g Fermentation broth of Bacillus Sanju 01 strain;

[0053] Arthrobacter nicotianae-16 (preservation number CGMCC NO.15402) was cultivated in beef extract medium, the fermentation temperature was 22-28°C, and the fermentation time was 2 days to obtain Arthrobacter nicotianae-16 with a viable count of 300 million / g fermentation broth;

[0...

Embodiment 2

[0059] 1. The tested variety is citrus, the variety is tangerine, and the planting period is 10 years. The tested varieties were transplanted to the same plot for the same management.

[0060] Soil for test: The soil in the test site is a stucco field, with 22.6g / kg of soil organic matter, 1.12g / kg of total nitrogen, 89.6mg / kg of alkaline nitrogen, 36.9mg / kg of available phosphorus, 102mg / kg of available potassium, and a pH value of 5.2. The soil fertility level is medium.

[0061] Carry out fertilization at transplanting stage (January 10), growing period (April 8), flowering stage (May 29), strong fruit stage (August 25) according to following treatment respectively:

[0062] Treatment 1: blank control, no fertilization;

[0063] Treatment 2: conventional fertilization;

[0064] Treatment 3: conventional fertilization+inactivation of the compound microbial preparation prepared in Example 1, using the compound microbial preparation prepared in Example 1 after inactivation ...

Embodiment 3

[0096] 1. The variety for testing: tobacco, the sample for testing: the compound microbial preparation prepared in Example 1.

[0097] From February 5th to March 15th, the ridges were raised according to the specification of 120cm, and the height of the ridges was more than 35cm. During the ridging process, base fertilizer was applied according to the conventional production requirements.

[0098] Soil for test: the soil in the test site is red soil, soil organic matter 19.6g / kg, total nitrogen 1.02g / kg, alkaline nitrogen 89.6mg / kg, available phosphorus 31.2mg / kg, available potassium 79.8mg / kg, pH value 5.3 , the soil fertility level is medium.

[0099] Treatment group 4 was treated with 50kg / 666.7m on March 30, 2018 (1 day before transplanting) 2 Apply the compound microbial preparation into the smoke hole, 50g per plant, and fertilize according to the routine. On April 13, the tobacco belt substrate was transplanted;

[0100] Simultaneously, treatment group 3 applied the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a compound microbial preparation and its application, and relates to the field of microbial technology. The compound microbial preparation of the present invention is prepared by a method comprising the following steps: inserting the fermentation liquid of Bacillus amyloliquefaciens Sanju-17 into straw for the second First ferment 15~20d, obtain the first fermented product; In the first fermented product, carry out the second fermentation after inoculating jelly-like Bacillus Sanju-01 strain fermented liquid and Arthrobacter tobacco Arthrobacter Sanju-16 fermented liquid, said The second fermentation time is 5-7 days to obtain the compound microbial preparation. The three biological strains in the compound microbial preparation of the present invention are all isolated by the inventors themselves. The compound microbial preparation provided by the present invention solves the problem that green agricultural preparations in the prior art have no mature products and has a large potential market gap. It has dual effects of pesticide and fertilizer, and can effectively reduce the frequency of fertilizer use and use cost when used. Significant environmental protection value, social benefit and economic benefit.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a composite microorganism preparation and its application. Background technique [0002] Since the 1970s, countries such as Europe, the United States, and Japan have successfully developed some composite bacterial agents, and many of them have begun large-scale production and formed serialized products. Among them, the EM bacteria agent developed by Professor Higa Teruo of the University of Ryukyu in Japan in the early 1980s is the most successful. It has been widely used in planting, breeding and environmental purification in more than 90 countries, and has produced significant economic and ecological benefits. and social benefits. [0003] A large number of studies have proved that the degradation or transformation of many organic substances requires the synergy of a variety of microorganisms to complete, and some important reactions that occur during the degradation pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/00C05F11/08C05F17/20C12R1/07C12R1/06
CPCC12N1/20C05F11/00C05F11/08C05F17/50Y02W30/40
Inventor 孙军伟范志勇户艳霞黄娟刘玉珍朱春苗郭小红骆毛喜林克明李肖宇余劲聪
Owner 漳州三炬生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com