Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism

An extracellular polymer and Bacillus technology, applied in the field of microorganisms, can solve the problems of non-biodegradation, high synthesis cost, non-regeneration, etc., and achieve the effects of easy cultivation, low cost, and preventing the formation of calcium scale

Pending Publication Date: 2019-08-27
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these substances are often non-biodegradable and non-renewable, and most organic corrosion inhibitors are toxic, easy to cause environmental pollution and high synthesis cost

Method used

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  • Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism
  • Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism
  • Preparation method and application of soluble extracellular polymeric substance of bacillus microorganism

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: This embodiment is the preparation and application of the soluble extracellular polymer of Bacillus cereus

[0037] The preparation method of the soluble extracellular polymer of microorganisms of the genus Bacillus, the specific steps are as follows:

[0038] (1) Adjust the pH value of the Luria-Bertani liquid medium to 7.0, place it at a temperature of 121 ° C for 30 minutes after high-temperature sterilization as a Luria-Bertani liquid fermentation medium, and use a solid Luria-Bertani medium to treat Bacillus cereus After the bacillus was activated for 24 hours, it was inoculated into a Luria-Bertani liquid fermentation medium and cultivated for 36 hours to obtain a Bacillus microbial fermentation liquid;

[0039] (2) centrifuging the Bacillus microbial fermentation liquid in step (1) at a temperature of 4° C., leaving it to stand and taking the supernatant, which is dialyzed through a dialysis bag to obtain a soluble extracellular polymer solution; wh...

Embodiment 2

[0057] Embodiment 2: This embodiment is the preparation and application of the soluble extracellular polymer of Bacillus cereus

[0058] The preparation method of the soluble extracellular polymer of microorganisms of the genus Bacillus, the specific steps are as follows:

[0059] (1) Adjust the pH value of the Luria-Bertani liquid medium to 7.4, place it at a temperature of 110°C for 35 minutes after high-temperature sterilization as a Luria-Bertani liquid fermentation medium, and use a solid Luria-Bertani medium to treat Bacillus cereus After the bacillus was activated for 30 hours, it was inoculated into a Luria-Bertani liquid fermentation medium and cultivated for 28 hours to obtain a Bacillus microbial fermentation liquid;

[0060] (2) centrifuging the Bacillus microbial fermentation liquid in step (1) at a temperature of 5° C., leaving it to stand to take the supernatant, which is dialyzed through a dialysis bag to obtain a soluble extracellular polymer solution; wherein...

Embodiment 3

[0063] Embodiment 3: This embodiment is the preparation and application of the soluble extracellular polymer of Bacillus cereus

[0064] The preparation method of the soluble extracellular polymer of microorganisms of the genus Bacillus, the specific steps are as follows:

[0065] (1) Adjust the pH value of the Luria-Bertani liquid medium to 8.0, and place it at a temperature of 100°C for high-temperature sterilization for 40 minutes as the Luria-Bertani liquid fermentation medium, using a solid Luria-Bertani medium to treat spores of cereus After the bacillus was activated for 48 hours, it was inoculated into a Luria-Bertani liquid fermentation medium and cultivated for 24 hours to obtain a Bacillus microbial fermentation liquid;

[0066] (2) Centrifuge the Bacillus microbial fermentation liquid in step (1) at a temperature of 8° C., leave it to stand to take the supernatant, and dialyze the supernatant through a dialysis bag to obtain a soluble extracellular polymer solution...

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Abstract

The invention discloses a preparation method and application of a soluble extracellular polymeric substance of a bacillus microorganism, and belongs to the technical field of microorganisms. The preparation method comprises the following steps: adjusting the pH (Potential of hydrogen) value of a Luria-Bertani liquid culture medium to be 7.0-8.0, performing high temperature sterilization and then using the treated Luria-Bertani liquid culture medium as a Luria-Bertani liquid fermentation culture medium, adopting a solid Luria-Bertani culture medium, performing activation culture on bacillus for24-48 h, then inoculating the Luria-Bertani liquid fermentation culture medium with the bacillus subjected to the activation culture, and culturing for 24-36 h to obtain a bacillus microorganism fermentation liquor; placing the bacillus microorganism fermentation liquor under the condition of the temperature of 4-8 DEG C and performing centrifugal treatment, standing and taking supernatant liquor, and dialyzing the supernatant liquor through a dialysis bag to obtain a soluble extracellular polymeric substance solution; performing freeze drying on the soluble extracellular polymeric substancesolution to obtain soluble extracellular polymeric substance powder; dissolving the soluble extracellular polymeric substance powder in distilled water to prepare a soluble extracellular polymeric substance water solution with a preset concentration for standby use. The soluble extracellular polymeric substance provided by the invention is used as a dual-functional biological corrosion inhibitor and scale inhibitor.

Description

technical field [0001] The invention relates to a preparation method and application of a soluble extracellular polymer of microorganisms belonging to the genus Bacillus, belonging to the technical field of microorganisms. Background technique [0002] Scaling is one of the main problems plaguing the use of industrial cooling circulation equipment and seawater desalination equipment. This is also a major concern in some industrial processes, namely energy production. Dirt covering the metal surface will not only lead to under-deposit corrosion, but also greatly reduce its heat transfer efficiency, cause energy loss and waste, and even bring many safety accidents. In France, non-productive expenditures related to scale-up are estimated at 1.5 billion euros per year; the same costs are about $800 million in the UK, $3 billion in Japan, and $9 billion in the United States; Caused economic losses of more than 70 billion yuan, far exceeding the world average [4] ; In addition,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12P19/34C12P19/04C12P13/02C23F11/10C02F5/12C12R1/085C12R1/11C12R1/125C02F103/02C02F103/08
CPCC12P21/00C12P19/34C12P19/04C12P13/02C02F5/12C23F11/10C02F2303/08C02F2103/023C02F2103/08
Inventor 屈庆李顺灵李蕾
Owner YUNNAN UNIV
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