Manufacture method of frozen section for observing handeliodendron bodinieri seed oil body

Abstract

The present invention discloses a manufacture method of a frozen section for observing handeliodendron bodinieri seed oil body, which comprises the following steps: (1) handeliodendron bodinieri seedsare taken and the middle parts of two cotyledons are cut respectively; (2) embedding: pre-cooling is performed; after the temperature is reduced to -30+/-2 DEG C, an embedding agent is dripped and refrigeration is performed for 40+/3 minutes; (3) serial section is performed and the thickness of the section is 15+/-2 mum; (4) the cut tissue is adhered to a glass slide; the part of the glass slideadhered to the cut tissue is immersed in water; and the tissue section goes away from the glass slide by shaking and is expanded in water; (5) the section expanded in water is dragged onto the glass slide; a dye solution is added dropwise and a cover glass covers on the section; then the frozen section for observing the handeliodendron bodinieri seed oil body is obtained. With the frozen section obtained by the method of the invention, the handeliodendron bodinieri seed oil body is clearly visible under a microscope; the integrity of the cotyledonstorage cell is good; cells basically do not overlap; and the section has a superior integrity, is extended and not easy to curl.

Description

technical field [0001] The invention relates to a method for making a frozen section, in particular to a method for making a frozen section for observing the oil body of the palm leaf seed kernel. Background technique [0002] Oil body, also known as liposome or oleosome (Dong Jinsong, 2009), is often stored in seed cotyledon or endosperm, and is surrounded by semi-unit membranes of liquid triacylglycerols , TAG) lipids form subcellular microdroplets (Huang A HC, 1992; Tzen et al., 1992), which is the basic unit of vegetable oil storage. Because the oil body is stable and not easily fused or aggregated by external influences (Frandsen et al., 2001), it is often used to observe the growth and development of vegetable oils at the cellular level and to identify the differences between high and low oil content varieties (Jang Sen 2015 ; Chen Hong, 2015; Luo Liping 2014). At present, ultrathin section and transmission electron microscope or scanning electron microscope (Tzen et...

AI Technical Summary

Problems solved by technology

At present, ultrathin section and transmission electron microscope or scanning electron microscope (Tzen et al., 1993; Guo Wei et al., 2016), freehand section and laser confocal microscope (Dong Jinsong, 2009; ) or paraffin section and Optical microscopy (Zhao Na, 2015) for research, these methods have the characteristics of high equipment requirements or too cumbersome production steps, it is difficult to popularize and popularize, which restricts the research on key issues such as the correlation between oil body development and oil c...

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