Method for preparing cord blood mononuclear cells
A nuclear cell and umbilical cord blood technology, applied in the cell-related field, can solve the problems of unguaranteed safety in premature infants, unusable application in premature infants, and low cell recovery rate, so as to facilitate infusion and use, avoid bacterial contamination, and cell recovery. active effect
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[0023] The preparation method of embodiment 1 umbilical cord blood mononuclear cells
[0024] (1) Add 28ml of anticoagulant to 27ml of cord blood collected, mix thoroughly and transfer to a sterile triple bag, in which the whole blood mononuclear cells (MNC) 10 6 / ml is 3.1, whole blood mononuclear cells (MNC) 10 8 / ml is 1.705;
[0025] (2) Centrifuge the transferred umbilical cord blood under the conditions of temperature 20°C, centrifugal force 500G, and centrifugation time 13 minutes;
[0026] (3) Put the centrifuged blood bag on the separation clamp to remove the plasma supernatant, and the precipitate is the mononuclear cell preparation. After centrifugal concentration, the umbilical cord blood mononuclear cell preparation is 10ml, and the whole blood mononuclear cell ( MNC)10 6 / ml is 16.28, whole blood mononuclear cells (MNC) 10 8 / ml is 1.628.
[0027] The only difference between Examples 2-30 and Example 1 is that the amount of cord blood collected is different,...
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