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High performance liquid chromatography method for determining residual amount of ningnanmycin in rice

A high-performance liquid chromatography and ningnamycin technology, which is applied in the field of high-performance liquid chromatography detection for the determination of ningnamycin residues in rice, can solve the problems of inability to be widely popularized and applied, high operational requirements for test personnel, and high detection costs. , to achieve the effect of efficient and fast purification operation, reduced detection cost and good repeatability

Inactive Publication Date: 2019-09-20
中山永恒检测科技有限公司
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Problems solved by technology

Wang Fang and others disclosed the detection method and conditions for the determination of Ningnanmycin content by liquid chromatography, but did not involve the extraction and detection of actual samples; Zha Yubing et al. invented a method for the determination of Ningnanmycin content in tomato using ultra-high performance liquid chromatography tandem mass spectrometry combined with QuEChERS sample purification technology. High, unable to widely promote the application

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  • High performance liquid chromatography method for determining residual amount of ningnanmycin in rice

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[0021] The scheme of the present application is further described in conjunction with specific examples as follows:

[0022] A high-performance liquid chromatography detection method for measuring Ningnanmycin residue in rice, comprising the following steps:

[0023] (1) Preparation of standard solution: Accurately draw 10 μL of 1000mg / L Ningnanmycin standard solution, dilute with methanol to 1mL, and then dilute with mobile phase to prepare a series of Ningnanmycin standard solutions with concentration gradients for high-efficiency Liquid chromatography assay.

[0024] (2) Extraction and purification: Weigh 10g of uniform sample into a 50mL stoppered centrifuge tube, add 20mL of acidified methanol solution (adjust the pH to 4.5 with acetic acid), 6g of anhydrous magnesium sulfate, and 1g of sodium chloride. / min high-speed centrifugation for 10 min, take 10 mL of the supernatant and blow it to near dryness with nitrogen at 40 °C, redissolve with the mobile phase to 1 mL, pas...

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Abstract

The invention provides a high performance liquid chromatography method for determining the residual amount of ningnanmycin in rice. The method comprises the steps that a uniform sample of 5 to 10g is weighed and put in a 50 mL stoppered centrifuge tube; an acidified methanol solution with the pH of 4.2 to 4. 6, anhydrous magnesium sulfate and sodium chloride are added for vortexing vibrating extraction for 2 to 5min; high-speed centrifugation is carried out for 5 to 10min; a supernatant liquid of 5 to 10mL is blown by nitrogen at 40 DEG C to near dry; moving phase redissolving is carried out with a dropper to bring the bottom of the meniscus to 1 mL; and through a 0.22-micron water-based microporous filter membrane, high performance liquid chromatography is carried out. According to the invention, the method can quantitatively detect Ningnanmycin in rice easily, quickly, accurately and sensitively; and indicators such as detection limit, recovery rate and reproducibility can all meet the relevant provisions of the national standard.

Description

technical field [0001] The invention belongs to the field of determination of pesticide residues, and relates to a high-performance liquid chromatography detection method for determining the residual amount of Ningnanmycin in rice. Background technique [0002] Ningnanmycin is a new type of high-efficiency, low-toxicity, low-residue, broad-spectrum biological pesticide successfully developed by the Chengdu Institute of Biology, Chinese Academy of Sciences. It is a fermentation product of Streptomyces noursii var. Glycoside-type antibiotic fungicides. Ningnanmycin can systematically induce plants to produce PR protein, reduce the concentration of virions in plants, and destroy the structure of virions, so as to achieve the effect of preventing and treating viral diseases in crops. With its excellent bactericidal performance, Ningnanmycin has been widely used in the field of pollution-free fruit and vegetable cultivation, and its potential market prospects are immeasurable. ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14
Inventor 陈晓伟陈丽红熊幸
Owner 中山永恒检测科技有限公司
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