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Natural antibacterial polyphenol compound, preparation method thereof and application of natural antibacterial polyphenol compound in electronic cigarettes

A polyphenolic compound, a natural antibacterial technology, applied in the field of phytochemistry, can solve the problems of less research on polyphenolic compounds, achieve good application prospects, good antifungal activity, and increase the shelf life effect

Inactive Publication Date: 2019-09-24
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the polyphenolic compounds contained in it. For this reason, the study of polyphenolic compounds and their activities in bark willow bark can provide new ideas for the utilization of bark willow bark.
Compound of the present invention has not been reported so far

Method used

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  • Natural antibacterial polyphenol compound, preparation method thereof and application of natural antibacterial polyphenol compound in electronic cigarettes
  • Natural antibacterial polyphenol compound, preparation method thereof and application of natural antibacterial polyphenol compound in electronic cigarettes
  • Natural antibacterial polyphenol compound, preparation method thereof and application of natural antibacterial polyphenol compound in electronic cigarettes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A preparation method of natural antibacterial polyphenolic compounds, comprising the steps of:

[0047] Step (1), extraction of extract: crush the dried willow bark into 30-50 mesh, use 70% ethanol aqueous solution as the extraction solvent, extract at room temperature, filter the extract, and concentrate the filtrate under reduced pressure to obtain extract;

[0048]Step (2), one-time silica gel column chromatography: apply the extract obtained in step (1) to silica gel column chromatography, and perform gradient elution with a mixed organic solvent of petroleum ether and acetone, and the volume ratio is 10:1, 10:3 in sequence , 10:5, 10:6, 10:7, 10:9, and 10:10, each gradient eluted to the TLC plate without spots, then replaced the next gradient elution; collected the gradient elution of each gradient and Concentrated and monitored by TLC, the same fractions were combined to obtain seven fractions A1-A7;

[0049] Step (3), secondary silica gel column chromatography:...

Embodiment 2

[0052] A preparation method of natural antibacterial polyphenolic compounds, comprising the steps of:

[0053] Step (1), extraction of extract: crush the dried willow bark into 30-50 mesh, use 95% ethanol aqueous solution as the extraction solvent, extract at room temperature, filter the extract, and concentrate the filtrate under reduced pressure to obtain extract;

[0054] Step (2), one-time silica gel column chromatography: apply the extract obtained in step (1) to silica gel column chromatography, and perform gradient elution with a mixed organic solvent of petroleum ether and acetone, and the volume ratio is 10:1, 10:3 in sequence , 10:5, 10:6, 10:7, 10:9, and 10:10, each gradient eluted to the TLC plate without spots, then replaced the next gradient elution; collected the gradient elution of each gradient and Concentrated and monitored by TLC, the same fractions were combined to obtain seven fractions A1-A7;

[0055] Step (3), secondary silica gel column chromatography...

Embodiment 3

[0063] A preparation method of natural antibacterial polyphenolic compounds, comprising the steps of:

[0064] Step (1), extraction of extract: crush the dried willow bark into 30-50 mesh, use 75% ethanol aqueous solution as the extraction solvent, extract at room temperature, filter the extract, and concentrate the filtrate under reduced pressure to obtain extract;

[0065] Step (2), one-time silica gel column chromatography: apply the extract obtained in step (1) to silica gel column chromatography, and perform gradient elution with a mixed organic solvent of petroleum ether and acetone, and the volume ratio is 10:1, 10:3 in sequence , 10:5, 10:6, 10:7, 10:9, and 10:10, each gradient eluted to the TLC plate without spots, then replaced the next gradient elution; collected the gradient elution of each gradient and Concentrated and monitored by TLC, the same fractions were combined to obtain seven fractions A1-A7;

[0066] Step (3), secondary silica gel column chromatography...

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Abstract

The invention relates to a natural antibacterial polyphenol compound, a preparation method thereof and application of the natural antibacterial polyphenol compound to electronic cigarettes, and belongs to the technical field of phytochemistry. The structural formula of the compound is shown as a formula (I), wherein the formula (I) is shown in the description. The compound is separated from barks of periploca sepium Bunge for the first time and determined to be the polyphenol compound through a nuclear magnetic resonance and mass spectrometry method, and the specific structure of the compound is characterized. The compound shows good inhibitory activity against bacteria such as phytophthora parasitica, fusarium asiaticum and alternaria alternata and good antifungal activity and has good application prospects. Meanwhile, the compound is used in electronic cigarette liquid, can have a good antibacterial effect, prolong the shelf life of the electronic cigarette liquid and reduce the excessive sweetness in the smoking process and is easy to apply and popularize.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a natural antibacterial polyphenol compound, a preparation method thereof, and an application in electronic cigarettes. Background technique [0002] Polyphenols refer to a group of chemical substances in plants, named for their multiple phenolic groups. There are many types of polyphenols with different structures, and their bioavailability, antioxidant properties and effects on the human body are also different. Polyphenolic compounds can be roughly divided into flavonoids, stilbenes, phenolic acids and lignans according to their structures. [0003] Due to their good antioxidant activity, polyphenolic compounds can work together with other antioxidants such as vitamin E and carotene to exert antioxidant effects in the body, and remove substances harmful to human health-free radicals. Therefore, it has attracted more and more attention from the scientific c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C43/23A24F47/00C07C41/34A01N31/16A01P3/00A01P1/00
CPCA01N31/16A24F47/00C07C43/23
Inventor 朱东来赵伟洪鎏张霞巩效伟李寿波李廷华韩熠
Owner CHINA TOBACCO YUNNAN IND
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