Method for synthesizing silver nanoparticles through pseudomonas poae acellular supernatant, product prepared by method and application of silver nanoparticles

A supernatant and cell-free technology, applied in the field of green biosynthetic nanomaterials, can solve problems such as ineffective effects, and achieve the effects of low price, small average particle size, and stable structure

Active Publication Date: 2019-09-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to control the disease, many compounds such as chlorine dioxide glutaraldehyde, bisalmonium chloride, cefdonium chloride, etc. have been applied in field trials, but the effect is not obvious

Method used

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  • Method for synthesizing silver nanoparticles through pseudomonas poae acellular supernatant, product prepared by method and application of silver nanoparticles
  • Method for synthesizing silver nanoparticles through pseudomonas poae acellular supernatant, product prepared by method and application of silver nanoparticles
  • Method for synthesizing silver nanoparticles through pseudomonas poae acellular supernatant, product prepared by method and application of silver nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Bacterial culture: Pick a single colony of Pseudomonas phlegm on the NA agar plate and put it in a 5ml test tube culture medium. After overnight shaking culture at 30°C and 200rpm, transfer it to NB liquid medium at a ratio of 1%. overnight culture at 30°C with a shaking rate of 200rpm;

[0043] (2) Collection of supernatant: centrifuge the bacterial suspension obtained in step (1) at 8000 rpm for 10 minutes and collect the supernatant to obtain cell-free supernatant (CFCS);

[0044] (3) Preparation of nano silver: get the bacterial supernatant obtained in step (2) and 1mM AgNO 3 The solution was fully mixed according to the ratio of 1:3, put into a 200rpm shaker and shaken in the dark for 48h, then the obtained reaction solution was passed through 8000rpm, centrifuged for 15min, washed 3 times with distilled water, and vacuum freeze-dried to obtain nano silver particles.

Embodiment 2

[0046](1) Bacterial culture: Pick a single colony of Pseudomonas phlegm on the NA agar plate and put it in a 5ml test tube culture medium. After overnight shaking culture at 30°C and 200rpm, transfer it to NB liquid medium at a ratio of 1%. overnight culture at 30°C with a shaking rate of 200rpm;

[0047] (2) centrifuge the bacterial suspension obtained in step (1) at 10000 rpm for 10 min and collect the supernatant to obtain a cell-free supernatant (CFCS);

[0048] (3) take the bacterial supernatant obtained in step (2) and 0.5mM AgNO 3 The solution was fully mixed according to the ratio of 1:5, and placed in a 200rpm shaker for 48h in the dark. Then the obtained reaction solution was subjected to 8000 rpm, centrifuged for 10 min, washed with distilled water for 3 times, and vacuum freeze-dried to obtain silver nanoparticles.

Embodiment 3

[0050] (1) Bacterial culture: Pick a single colony of Pseudomonas phlegm on the NA agar plate and put it in a 5ml test tube culture medium. After overnight shaking culture at 30°C and 200rpm, transfer it to NB liquid medium at a ratio of 1%. overnight culture at 30°C with a shaking rate of 200rpm;

[0051] (2) centrifuge the bacterial suspension obtained in step (1) at 9000 rpm for 15 minutes and collect the supernatant to obtain a cell-free supernatant (CFCS);

[0052] (3) get the bacterial supernatant that step (2) obtains and 1.5mM AgNO 3 The solution was fully mixed according to the ratio of 1:4, and placed in a 200rpm shaker for 48h in the dark. Then the obtained reaction solution was subjected to 10000 rpm, centrifuged for 15 min, washed with distilled water for 3 times, and vacuum freeze-dried to obtain silver nanoparticles.

[0053] The performance and bacteriostatic effect of the nano-silver particles obtained through the mediation of Pseudomonas phlegm in Example 1...

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Abstract

The invention discloses a method for synthesizing silver nanoparticles through pseudomonas poae acellular supernatant. The method comprises the following steps of (1) culturing pseudomonas poae in NB culture mediums, and collecting bacterium suspension liquid; (2) centrifuging the bacterium suspension liquid, and collecting supernatant so as to obtain acellular supernatant; and (3) adding the acellular supernatant to an AgNO3 solution, performing centrifuging on obtained reaction liquid, performing washing, and performing vacuum freeze drying so as to obtain silver nanoparticle granules. The prepared silver nanoparticle granules are stable in structure and good in dispersibility, have good biological compatibility, have high in vitro and in vivo antibacterial activity on Dickeya dadantii CZ1501, and can be prepared into ecology-friendly type gold nanoparticle bacteriostatic agents good in biocompatibility to be used for preventing and treating stem rot of sweet potatoes, burden of traditional chemical bactericides to environment is alleviated, and the method has favorable popularization prospects.

Description

technical field [0001] The invention belongs to the technical field of green biosynthetic nanomaterials, and specifically relates to a method for synthesizing nanosilver by utilizing the cell-free supernatant of Pseudomonas phlegm and its application in agriculture. Background technique [0002] Nanomaterials refer to their structural units in the range of 1-100nm. Because of their unique physical and chemical properties, such as small size and large specific surface area, they have broad development potential in the fields of science and technology. Among them, silver nanoparticles (AgNPs) have strong antibacterial properties and have potential antibacterial activity against Gram-negative and Gram-positive bacteria such as Escherichia coli and Staphylococcus aureus. [0003] At present, the traditional methods of synthesizing nano-silver mainly include physical methods, chemical methods, and microbial methods. The use of physical methods and chemical methods is simple and f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00A01N59/16A01P3/00C12R1/38
CPCC12P3/00A01N59/16
Inventor 李斌洪纤纤张牧晨杨敏邱文张阳阿弗萨娜
Owner ZHEJIANG UNIV
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