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A kind of method of fermentative production Jinggangmycin

A technology of Jinggangmycin and Jinggang variants, which is applied in the field of fermentation and production of Jinggangmycin, can solve problems such as increased ventilation, energy waste, and difficulty in oxygen transfer, so as to reduce the use of sterile air, ensure oxygen supply, and shorten the fermentation cycle. Effect

Active Publication Date: 2020-09-11
ZHEJIANG TONGLU HUIFENG BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Jinggangmycin is an aminoglycoside compound. Rice flour is used as a fermentation medium in industrial production. After high-temperature sterilization, the rice flour has a high viscosity, which makes it difficult to transmit oxygen. In order to ensure the supply of oxygen, it is necessary to increase the ventilation rate, resulting in a waste of energy.
[0003] In order to solve this problem, it is possible to carry out liquefaction with liquefied amylase before the medium is sterilized (using rice flour liquefied and saccharified liquid to ferment Jinggangmycin A, pesticide, the 5th issue in 2003), the existing problem is that the foam is relatively large in the sterilization process. Many, difficult to control, and the liquefaction process needs to be carried out in a fermentation tank or a batching device. The liquefaction process requires a certain temperature and a certain time for the liquefaction process, which increases energy consumption, operating procedures and equipment occupation. difficulty

Method used

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  • A kind of method of fermentative production Jinggangmycin
  • A kind of method of fermentative production Jinggangmycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Inoculate the strain of Streptomyces hygroscopicus Jinggang variant into the liquid fermentation medium, control the inoculum size to 7%, and the fermentation temperature is 40°C; each liter of liquid fermentation medium contains 70g of rice flour, 30g of peanut cake powder, 2g of NaCl, and 2g of CaCl 2 2g, KH 2 PO 4 3g, the pH of the fermentation medium was 8.0. At the same time, take 1×10 3 19.0kg of U / g mesophilic α-amylase was sterilized by filtration through a 0.22 μm microporous membrane, and pumped into the fermenter by a peristaltic pump.

[0035] 2) During the fermentation process, the ventilation volume is controlled to 2500m 3 / h, stirring speed 120rpm.

[0036] 3) Cultivate for 45 hours, stop the fermentation, measure the unit potency, put the tank volume, record the electric meter value before and after the test, and calculate the Jinggangmycin fermentation yield and electricity consumption.

[0037] As a result, the titer was 22600ug / mL, the tank ...

Embodiment 2

[0039] 1) Inoculate Streptomyces hygroscopicus varietal strain Jinggang into the liquid fermentation medium, control the inoculation amount to 10%, and the fermentation temperature is 38°C; each liter of liquid fermentation medium contains 120g of rice flour, 20g of peanut cake powder, 1g of NaCl, and 1g of CaCl 2 1g, KH 2 PO 4 1g, the pH of the fermentation medium is 7.0. At the same time, take 5×103 6.5 kg of mesophilic α-amylase in U / g was sterilized by filtration through a 0.22 μm microporous membrane, and pumped into the fermenter by a peristaltic pump.

[0040] 2) During the fermentation process, the ventilation volume is controlled to 3000m 3 / h, stirring speed 100rpm.

[0041] 3) Cultivate for 45 hours, stop the fermentation, measure the unit potency, put the tank volume, record the electric meter value before and after the test, and calculate the Jinggangmycin fermentation yield and electricity consumption.

[0042] As a result, the titer was 24800ug / mL, the tan...

Embodiment 3

[0044] 1) Inoculate Streptomyces hygroscopicus varietal strain Jinggang into the liquid fermentation medium, control the inoculum size to 12%, and the fermentation temperature is 40°C; every liter of liquid fermentation medium contains rice flour 100g, peanut cake powder 25g, NaCl 1.5g, CaCl 2 1g, KH 2 PO 4 2g, the pH of the fermentation medium was 7.2. At the same time, take 2×10 3 13.5 kg of mesophilic α-amylase in U / g was sterilized by filtration through a 0.22 μm microporous membrane, and pumped into the fermenter by a peristaltic pump.

[0045] 2) During the fermentation process, the ventilation volume is controlled to 3500m 3 / h, stirring speed 150rpm.

[0046] 3) Cultivate for 48 hours, stop the fermentation, measure the unit potency, put the tank volume, record the electric meter value before and after the test, and calculate the Jinggangmycin fermentation yield and electricity consumption.

[0047] As a result, the titer was 25100ug / mL, the tank volume was 42....

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Abstract

The invention provides a method for producing validamycin through fermentation, and belongs to the technical field of antibiotic production. The method comprises the following steps that a fermentation strain is inoculated into a fermentation culture medium, and medium-temperature alpha-amylase is added for fermentation, wherein the fermentation strain is streptomyces tygroscopicus, and the preservation number of the streptomyces tygroscopicus is CGMCC No.6636; the enzyme active unit of the medium-temperature alpha-amylase is (1-5)*10<3>U / g; the mass ratio of the medium-temperature alpha-amylase to the fermentation culture medium is 1:(166-833). According to the method, on the premise without affecting the yield of the validamycin, the cost is reduced, the fermentation period is shortened,the sterile air using amount can be greatly reduced, the electricity consumption is reduced, and energy sources are saved.

Description

technical field [0001] The invention belongs to the technical field of antibiotic production, in particular to a method for producing Jinggangmycin by fermentation. Background technique [0002] Jinggangmycin, also known as effective mycin, is an antibiotic produced by actinomycetes. It has strong systemic properties, is easily absorbed by bacterial cells and conducts rapidly in it, interfering with and inhibiting the growth and development of bacterial cells. It is a low toxicity fungicide. Jinggangmycin is an aminoglycoside compound. Rice flour is used as a fermentation medium in industrial production. After high-temperature sterilization, the rice flour has a high viscosity, which makes it difficult to transmit oxygen. To ensure the supply of oxygen, it is necessary to increase the ventilation rate, resulting in a waste of energy. [0003] In order to solve this problem, it is possible to carry out liquefaction with liquefied amylase before the medium is sterilized (usin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/46C12R1/55
CPCC12P19/46
Inventor 殷红福王佳李忠倪烈
Owner ZHEJIANG TONGLU HUIFENG BIOSCI